Colorimetric analysis method for quantitatively detecting copper ions through fluorescent equipment

A copper ion, fluorescence ratio technology, applied in fluorescence/phosphorescence, material excitation analysis, etc., can solve the problems of insufficient consideration of indicator fixation, unsatisfactory detection sensitivity, cumbersome production process, etc. Rapid image acquisition results

Active Publication Date: 2014-01-15
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Abstract
  • Description
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Problems solved by technology

At present, there are also some commercial copper ion detection test papers on the market, some of which are prepared by soaking the organic solution containing the indicator and drying it. The fixation of the indicator is not fully considered, which leads to the easy decolorization of the test paper; The indicator is prepared by mixing with the pulp, the production process is cumbersome, and the detection sensitivity is not ideal

Method used

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  • Colorimetric analysis method for quantitatively detecting copper ions through fluorescent equipment
  • Colorimetric analysis method for quantitatively detecting copper ions through fluorescent equipment
  • Colorimetric analysis method for quantitatively detecting copper ions through fluorescent equipment

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Embodiment Construction

[0033] Establishment of standard color card and standard concentration curve

[0034] Specifically:

[0035] 1) Preparation of indicator solution: Dissolve rhodamine fluorescent indicator in ethanol solution according to a certain mass fraction, and then dilute it with ethanol and water (volume ratio: alcohol / water=7 / 3) to make the indicator The final concentration is 5×10 -4 M.

[0036] 2) Preparation of copper standard solution: Wahaha drinking pure water was used to prepare the concentration of 1.6×10 -3 M, 3.2×10 -3 M, 4.8×10 -3 M, 6.4×10 -3 M, 8×10 -3 M, 1.6×10 -2 M, 4×10 -2 M, 8×10 -2 A series of copper ion standard solutions of M;

[0037] 3) Reaction between the indicator and copper ions: Add 1mL of the indicator solution in ① above into a 1.5mL plastic small tube, take 12.5μL of the copper standard solution in ② into the plastic small tube, mix well and place at 70°C React in a constant temperature water bath for 30 minutes to obtain a red solution with a s...

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Abstract

The invention relates to fluorescent colorimetric analysis and discloses a colorimetric analysis method for quantitatively detecting copper ions through fluorescent equipment. The colorimetric analysis method comprises the following steps: reacting a copper ion solution and a Rhodamine fluorescent indicator synthesized in a lab to enhance fluorescence, and exciting the indicator through light with a particular wavelength of 365nm by adopting home-made fluorescent colorimetric equipment; catching fluorescence color change of the indicator before and after the copper ions are added by using electronic imaging equipment such as a scanner and a digital camera; extracting color change values of red, green and blue (RGB) channels in the image before and after color change, and establishing a standard curve between the copper ions and a concentration gradient which corresponds to the copper ions; and comparing the obtained color change values of the sample with values in the curve in a sample test process, thus quantitatively and selectively analyzing the copper ions in an unknown sample. According to the method, copper ion concentration of 10<-5>M can be detected, co-existing interference of nine ions can be effectively eliminated, and the method has high selectivity.

Description

technical field [0001] The invention relates to a fluorescence colorimetric method, in particular to a colorimetric analysis method for quantitative detection of copper ions by fluorescence equipment. Background technique [0002] Copper, because of its excellent mechanical strength, good ductility, super memory ability, and stable chemical properties, is widely used in industries such as steel, metallurgy, paint, national defense, electronics, chemistry, and ceramics. A revolutionary change has come. All kinds of copper pollution are also lost to the environment, posing a threat to people's lives. Although copper is an essential metal element for human life activities, the possible copper requirement per person per day is 25-35 μg. Copper participates in the composition and metabolism of enzymes in the body, can promote insulin secretion, and lower blood sugar. However, the harm caused by excessive copper to the human body cannot be ignored. Excessive copper intake can l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 冯亮关亚风李慧吕永军李笑
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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