Peanut high-oleic-acid character gene selection method

Abstract

The invention discloses a peanut high-oleic-acid character gene selection method. The method comprises the steps of extracting leaf gene groups of different peanut breeds, and amplifying AhFAD2A and AhFAD2B genes from the gene groups by using a specificity primer; cutting and recycling specificity amplification stripes and sequencing to obtain the sequence of the gene; translating the obtained sequence, comparing and analyzing, and searching the point mutation or inserting site to judge the oleic acid content and specific value of oleic acid / linoleic acid (O / L) of the breed. The method is simple to operate, variety of peanuts germ plasm can be analyzed, the oleic acid content of single plant of peanut can be determined in the seedling stage, the breeding process can be shortened and the breeding effect of oleic acid can be improved by combining comprehensive agronomic character selection of the single plant.

Description

technical field

[0001] The invention relates to a method for selecting genes for peanut high oleic acid traits, and belongs to the field of peanut biotechnology. Background technique

[0002] my country is the world's largest peanut producer, consumer and exporter. Peanut has an important position and role in my country's national economy. Oleic acid is the main fatty acid in peanut oil, accounting for 39%-49% of the total peanut fatty acid. It can reduce harmful cholesterol in human lipid metabolism, but maintain the level of beneficial cholesterol, thereby slowing down atherosclerosis, effectively Prevent the occurrence of cardiovascular and cerebrovascular diseases such as coronary heart disease. In addition, the content of oleic acid is high, the anti-oxidation ability is strong, the product is not easy to oxidative rancidity, the shelf life is long, and the storage capacity is also strong. Therefore, the breeders of the world's major oil crops (soybean, rapeseed, pea...

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