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Method for producing sophorolipid through fermentation by taking cottonseed molasses and cottonseed oil as substrates

A technology of cottonseed molasses and cottonseed oil, which is applied in the field of sophorolipid production, to achieve the effects of improving utilization rate, simple process, and benefiting large-scale industrial production

Inactive Publication Date: 2014-01-29
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to literature and patent searches, the research on the production of sophorolipids using cottonseed molasses and cottonseed oil as cheap substrates has not been reported at home and abroad.

Method used

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  • Method for producing sophorolipid through fermentation by taking cottonseed molasses and cottonseed oil as substrates
  • Method for producing sophorolipid through fermentation by taking cottonseed molasses and cottonseed oil as substrates
  • Method for producing sophorolipid through fermentation by taking cottonseed molasses and cottonseed oil as substrates

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Replacement of the first carbon source and the second carbon source: Prepare four sets of fermentation media (g / l): a glucose 80.0, oleic acid 6% (v / v); b glucose 80.0, cottonseed oil 6% (v / v); c cottonseed molasses 133.33 (sugar 80.0), oleic acid 6% (v / v), d cottonseed molasses 133.33 (sugar 80.0), cottonseed oil 6% (v / v), each group added KH 2 PO 4 1.0, Na 2 HPO 4 1.0, MgSO 4 ·7H 2 O0.50, yeast powder 3.0, pH natural.

[0033] (2) Production of sophorolipids by fermentation: Sterilize the four groups of fermentation media in (1) at 115°C for 30 minutes, then cool. Inoculate the activated Wickerhamierlla domercqiae var.sophorolipid CGMCC No.1576 into the above four fermentation media at 25°C and 200rpm at an inoculation volume of 2% by volume, and ferment on a shaking table for 7 One day, the fermentation broth of sophorolipids was obtained.

[0034] (3) Crude extraction of sophorolipids: take the fermentation broth containing sophorolipids and add 2 times ...

Embodiment 2

[0038](1) Nitrogen source gradient design: Prepare five sets of fermentation media (g / l): the amount of yeast powder added is 0, 1.0g / l, 2.0g / l, 3.0g / l, 4.0g / l each gradient, and added cottonseed molasses 133.33 (sugar 80.0), KH 2 PO 4 1.0, Na 2 HPO 4 1.0, MgSO 4 ·7H 2 O0.50, cottonseed oil 6% (v / v), pH natural.

[0039] (2) Production of sophorolipids by fermentation: Sterilize the five groups of fermentation media in (1) at 115°C for 30 minutes, then cool. The activated Wickerhamierlla domercqiae var.sophorolipid CGMCC No.1576 was inoculated into the above-mentioned fermentation medium at 25°C and 200rpm at an inoculum size of 2% by volume, and the shaker was shaken for 7 days. A fermented broth of sophorolipids is obtained.

[0040] (3) Crude extraction of sophorolipids: take the fermentation broth containing sophorolipids and add 2 times its volume of ethyl acetate to extract, centrifuge at 6000rpm for 15min, collect the ethyl acetate layer, concentrate under reduce...

Embodiment 3

[0044] (1) Influence of inorganic salts: prepare two sets of fermentation media (g / l): add KH to one set 2 PO 4 1.0, Na 2 HPO 4 1.0, MgSO 4 ·7H 2 O0.50, the other group did not add inorganic salt ions, both groups added cottonseed molasses 133.33 (sugar 80.0), cottonseed oil 6% (v / v), pH natural.

[0045] (2) Production of sophorolipids by fermentation: Sterilize the two groups of fermentation media in (1) at 115°C for 30 minutes, then cool. The activated Wickerhamierlla domercqiae var.sophorolipid CGMCC No.1576 was inoculated into the above-mentioned fermentation medium at 25°C and 200rpm at an inoculum size of 2% by volume, and the shaker was shaken for 7 days. A fermented broth of sophorolipids is obtained.

[0046] (3) Crude extraction of sophorolipids: take the fermentation broth containing sophorolipids and add 2 times its volume of ethyl acetate to extract, centrifuge at 6000rpm for 15min, collect the ethyl acetate layer, concentrate under reduced pressure and dry...

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Abstract

The invention discloses a method for producing sophorolipid through fermentation by taking cottonseed molasses and cottonseed oil as the substrates, wherein expensive glucose and oleic acid are replaced by cheap cottonseed molasses and cottonseed oil to produce sophorolipid by fermentation. The method disclosed by the invention has the characteristics of being low in raw material cost, simple and convenient in process and high in sophorolipid production capability. According to the method, the basis is provided for increasing the utilization rate of cottonseed, reducing energy consumption and popularizing large-scale industrial production of the sophorolipid.

Description

technical field [0001] The invention relates to a method for producing sophorolipids, in particular to a method for producing sophorolipids by fermenting Wickerhamiella domercqiae var. sophorolipid with cottonseed molasses and cottonseed oil as substrates. Background technique [0002] Sophorolipid is an important surfactant. Compared with chemical surfactants, it has the advantages of biodegradability, low toxicity and good environmental compatibility, and is widely used in petroleum, food, medicine, environmental Protection, cosmetics and other industrial fields. [0003] Sophorolipids are currently mainly obtained through microbial fermentation. The high cost of preparation is an important factor restricting the application of sophorolipids. Therefore, trying to reduce the production cost of sophorolipids and increase the yield of sophorolipids is a prerequisite for its wide application in various fields. [0004] China is a major cotton-producing country in the world,...

Claims

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Application Information

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IPC IPC(8): C12P19/44C12R1/645
Inventor 宋欣
Owner SHANDONG UNIV
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