6results about How to "Good alternative" patented technology

The present invention relates to the field of bioengineering technology, and in particular to a single-cell green algae combined functional factor and its application in cell-cultured meat. The functional factor is obtained by subjecting the raw material to processes such as low-temperature freezing, room temperature thawing, and nano-scale shell adsorption column refining. The extraction process is simple and highly safe, and is suitable for industrial scale-up production. When applied to muscle cell culture, it can be directly dissolved in the basal culture medium, and the compounding method with the culture medium is simple. It has no toxic side effects on muscle cell growth and can completely replace serum for muscle cell culture. When the single-cell green algae combined functional factor mentioned in the present invention is applied to fish muscle cell culture, compared with the group without serum addition, it can provide cells with growth functional factor supplements, promote cell proliferation and differentiation in vitro, and can be applied to the preparation of cell-cultured meat.

The present invention relates to the field of bioengineering technology, and in particular to a blue algae water extract and its use as a substitute for serum. The water extract is obtained by processes such as raw material extraction, low-temperature freezing, room temperature thawing, and purification by nano-scale shell adsorption columns of blue algae. The extraction process is simple and highly safe, and is suitable for industrial scale-up production. When applied to muscle cell culture, it can be directly dissolved in the basal culture medium, and the compounding method with the culture medium is simple. It has no toxic side effects on muscle cell growth and can completely replace serum for muscle cell culture. The blue algae water extract mentioned in the present invention is applied to the muscle cell culture process to replace serum, which can meet the effect of long-term continuous subculture of cells in culture bottles, and can be compounded into a culture fluid and applied to a bioreactor to expand the culture of cell meat.

This invention relates to the field of feed production technology, specifically a method for preparing a substitute for corn feed. The preparation steps are as follows: S1, pretreated giant reed grass is placed in a mixed solution of water, ethanol, and acetic acid to obtain a primary filtrate and a primary filter residue; S2, the primary filtrate obtained in step S1 is mixed with pretreated edible mushroom residue to obtain a secondary filtrate and a secondary filter residue; S3, the secondary filter residue is mixed with water to obtain a mixture, and *Thiobacillus ferrooxidans* and *Thiobacillus thiooxidans* are added to the mixture for fermentation, followed by centrifugation and filtration to obtain a tertiary filter residue; S4, the tertiary filter residue is mixed with the primary filter residue and the secondary filtrate, and laccase, lactic acid bacteria, *Lactobacillus plantarum*, and yeast are added for fermentation to obtain the finished feed product. This invention prepares feed through the joint fermentation of edible mushroom residue and giant reed grass, which can effectively replace corn feed, and the feed has a low heavy metal content.

The utility model relates to a manual opening device of a magnetic control circuit breaker, which comprises a synchronizing shaft, a crank arm is fixedly arranged on the synchronizing shaft corresponding to a magnetic control mechanism, the crank arm is connected with an opening mechanism of the magnetic control mechanism through a connecting plate, the connecting plate is rotatably connected with the crank arm and the opening mechanism, one end of the synchronizing shaft is sleeved with a connecting sleeve, and the other end of the synchronizing shaft is sleeved with a connecting rod. A kidney-shaped through hole is formed in the side wall of the connecting sleeve, a connecting pin is installed in the kidney-shaped through hole and penetrates through the synchronizing shaft in the radial direction, and the outer end of the connecting sleeve is connected with an operating handle. The hand-operated switch-off device is simple in structure, can meet the requirement of hand-operated switch-off, and has a certain waterproof function.

The invention relates to the technical field of feed preparation, in particular to a feed for replacing corn and a preparation method thereof.The preparation method comprises the following preparation steps that S1, pretreated pennisetum sinese is put into a mixed solution of water, ethyl alcohol and acetic acid, and primary filtrate and primary filter residues are obtained; s2, stirring and mixing the primary filtrate obtained in the step S1 and the pretreated edible fungus residues to obtain secondary filtrate and secondary filter residues; s3, mixing the secondary filter residues with water to obtain a mixture, adding thiobacillus ferrooxidans and thiobacillus thiooxidans into the mixture for fermentation, and performing centrifugal filtration to obtain tertiary filter residues; and S4, mixing the third filter residue with the first filter residue and the second filter residue, and adding laccase, lactic acid bacteria, lactobacillus plantarum and saccharomycetes for fermentation to obtain a feed finished product. The feed is prepared through combined fermentation of the edible fungus residues and the pennisetum sinese, corn feed can be effectively replaced, and meanwhile the content of heavy metal in the feed is low.

The application discloses a method for producing prodigiosin by fermentation of sugarcane molasses and beet molasses. According to the Serratia marcescens PG-Zeno-001 strain developed by the company in the early stage, on the one hand, the by-product of the sugar industry, sugarcane molasses and beet molasses, is used as the carbon source for the production of prodigiosin by optimizing the formula of the fermentation medium; on the other hand, the method for producing prodigiosin by fermentation using the fermentation medium is provided, and in the large-scale fermentation of a 100L fermentation tank, the prodigiosin yield is up to 16.18 g / L at 48 h. The application can greatly reduce the cost of prodigiosin fermentation production, and is expected to build a new closed loop for the environment-friendly production of the sugar industry.