A kind of tissue culture rapid propagation method of bird's nest fern seedling

Abstract

The invention discloses a tissue culture method which is rapid in breeding speed and low in cost and can maintain leaves of asplenium antiquum osaka to be ordered and uniform and be in a waveform. The tissue culture method comprises the following steps: (1) inducing and differentiating a protocorm with a small grain shape from a root-shaped stem of the asplenium antiquum osaka; (2) taking the protocorm and putting into an MS (Murashige and Skoog) culture medium to be cultured, wherein each protocorm is differentiated into 3-4 new protocorms; carrying out multiplication culture of a sterile seedling; and (3) putting the protocorms into the MS culture medium to be cultured and growing 1-3 seedlings on each protocorm; putting each grown seedling on the other MS culture medium to be cultured to carry out transplantation domestication; and washing a culture solution on the roots of the sterile seedling and transplanting the sterile seedling into a base material composed of peat soil and perlite. The tissue culture method can be applied to the field of cultivation of asplenium nidus.

Description

technical field [0001] The invention relates to a plant cultivation method, in particular to a tissue culture and rapid propagation method for bird's nest fern seedlings. Background technique [0002] Bird's nest fern is an epiphytic fern native to southeastern Asia, eastern Australia, Indonesia, India, and eastern Africa. It is also called nest fern, shansu flower, and crown fern. Herbaceous foliage plants. In recent years, bird's nest fern has been popular among people in the application of foliage plants. In addition to natural potted plants used as fresh cut leaves for people to watch in flower arrangements, bird's nest ferns can also be artificially processed into various shapes, and arranged in different locations according to needs to form different landscape effects. This shows that people have higher and higher ornamental requirements for bird's nest fern. As a member of the bird's nest fern family, the wave-leaf bird's nest Victoria is very popular among people ...

AI Technical Summary

Problems solved by technology

But for bird's nest fern, about 30% of the spore propagation and the rapid propagation of seedlings using spores as raw materials cannot inherit the beautiful wave pattern of the bird's nest leaf itself, and the remaining 70% of the wave pattern is not very uniform. Big waves, small waves, so it needs to be screened during planting to get a neat and uniform wave leaf nest

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