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Difunctional nanoprobe for detecting mitochondrial cytochrome C as well as preparation method thereof

A cytochrome and nanoprobe technology, applied in the field of magnetic resonance imaging, can solve the problems of large rejection reaction of organisms and poor biocompatibility of nanoparticles, achieve good compatibility, improve biocompatibility and dispersibility Good results

Inactive Publication Date: 2015-04-29
张薇薇
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to this synthetic Fe 3 o 4 The biocompatibility of nanoparticles is poor, so after the probe containing the above nanoparticles is implanted into the organism, it will cause a large rejection reaction in the organism

Method used

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  • Difunctional nanoprobe for detecting mitochondrial cytochrome C as well as preparation method thereof
  • Difunctional nanoprobe for detecting mitochondrial cytochrome C as well as preparation method thereof
  • Difunctional nanoprobe for detecting mitochondrial cytochrome C as well as preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0029] This embodiment provides a method for preparing a bifunctional nanoprobe for detecting mitochondrial cytochrome C, comprising the following steps:

[0030] Step 101: Take the ligand of mitochondrial cytochrome C, and label the two ends with fluorescein and aldehyde respectively to obtain the first product;

[0031] Step 102: mixing the first product with magnetosomes and incubating overnight, then removing unreacted substances to obtain a second product;

[0032] Step 103: Add 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride, N-hydroxysuccinimide and methoxy-carboxylated-polyethylene diimide to the second product Alcohol, and incubated overnight, after removing unreacted substances, a dual-functional nanoprobe for detecting mitochondrial cytochrome c was obtained.

[0033] The reaction principle of the above method is:

[0034] Before the preparation of the probe, it has been known through characterization that the magnetosome is a ferromagnetic chain-like na...

Embodiment 2

[0044] This embodiment provides a method for preparing a bifunctional nanoprobe for detecting mitochondrial cytochrome C, comprising the following steps:

[0045] Step 201: Take the ligand of mitochondrial cytochrome C, and label the two ends with fluorescein and aldehyde respectively to obtain the first product;

[0046] Step 202: Mix the first product with magnetosomes at a ratio of 3:7 (mass ratio), incubate overnight, and then use a magnet to absorb the mixture to obtain an adsorbate, and then rinse the adsorbate to obtain a second product; wherein, the incubated The conditions are: under the condition of avoiding light, sonicate for 20s every 50-200s, and the power of the sonication is 20-80w.

[0047] Step 203: Add EDC, NHS and H to the second product 3 C-O-PEG-COOH, incubate overnight, and then use a magnet to absorb the mixture to obtain the adsorbate, and then rinse the adsorbate to obtain a dual-functional nanoprobe for detecting mitochondrial cytochrome C; the incu...

Embodiment 3

[0053] This embodiment provides a bifunctional nanoprobe for detecting mitochondrial cytochrome C, which can be prepared by any of the above methods.

[0054] In addition, in order to further illustrate the technical effects of the present invention, specific test examples are also provided below.

[0055] In this test example, for convenience of description, the bifunctional nanoprobe for detecting mitochondrial cytochrome C of the present invention is referred to as "PEG-cBMP" for short.

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Abstract

The invention relates to a difunctional nanoprobe for detecting mitochondrial cytochrome C as well as a preparation method thereof. The preparation method comprises the following steps: taking a ligand of the mitochondrial cytochrome C; labeling fluorescein and aldehyde group on two ends of the ligand respectively; then adding magnetosome into the ligand again, and incubating overnight; then removing substances which do not react; then adding 1-(3-dimethyl amino propyl)-3-ethylcarbodiimide hydrochloride, N-hydroxysuccinimide and methoxy-carboxylation-polyethylene glycol, and incubating overnight; and then removing substances which do not react, thereby obtaining the difunctional nanoprobe for detecting the mitochondrial cytochrome C. The difunctional nanoprobe and the preparation method thereof, which are provided by the invention, have good biocompatibility; the prepared probe for detecting mitochondrial cytochrome C has good dispersibility.

Description

technical field [0001] The invention relates to the field of magnetic resonance imaging, in particular to a bifunctional nanoprobe for detecting mitochondrial cytochrome C and a preparation method thereof. Background technique [0002] Techniques for detecting cells include a variety of techniques, such as magnetic resonance imaging. Magnetic resonance imaging uses the principle of magnetic resonance to detect the emitted electromagnetic waves through an external gradient magnetic field, and based on this, it can draw an image of the internal structure of the object. Most of the probes used contain superparamagnetic substances. At present, the superparamagnetic substances used in these probes are usually: artificially synthesized Fe 3 o 4 nanoparticles. Due to this synthetic Fe 3 o 4 Nanoparticles have poor biocompatibility, so after the probes containing the above-mentioned nanoparticles are implanted into organisms, the organisms will have a large rejection reaction. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/12
Inventor 张薇薇许乙凯
Owner 张薇薇