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Multifunctional magnetic nano particle and preparation method thereof

A magnetic nanoparticle and nanoparticle technology, which is applied in the fields of wave energy or particle radiation treatment materials, pharmaceutical formulations, preparations for in vivo tests, etc., can solve the problems of multifunctional nanoparticles without functional controllability

Inactive Publication Date: 2014-02-12
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the multifunctional nanoparticles prepared by the prior art do not have good functional controllability (the functional controllability refers to: different functional groups can be replaced according to actual needs, such as fluorescent reagents, targeting groups, drug molecules etc., realize the simultaneous implementation of tumor diagnosis and treatment)

Method used

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  • Multifunctional magnetic nano particle and preparation method thereof
  • Multifunctional magnetic nano particle and preparation method thereof
  • Multifunctional magnetic nano particle and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0045] Preparation of Multifunctional Magnetic Nanoprobes

[0046] (1) Fe 3 o 4 Preparation of Magnetic Nanoparticles

[0047] 0.360g (1.33×10 -3 mol) FeCl 3 ·6H 2 O, 0.133g (6.69×10 -4 mol) FeCl 2 4H 2 O and 1.621g (5.32×10 -3 mol) sodium oleate was successively added into a 25mL round bottom flask with 4mL ethanol, 3mL double distilled water and 7mL toluene mixture. The mixture was magnetically stirred under a nitrogen atmosphere, and refluxed at 74 °C for 4 h. After the reflux was completed, the flask was removed from the oil bath to cool to room temperature, and then ethanol was added to precipitate the product. The magnetic particles are obtained after centrifugation, and the obtained magnetic particles are redispersed in n-hexane, and the above steps are repeated 2-3 times, and the final product is redispersed in chloroform to prepare Fe 3 o 4 / CHCl 3 solution (10mg / mL).

[0048] (2) Fe 3 o 4 mSiO 2 Preparation of nanoparticles

[0049] 1mL Fe 3 o 4 / ...

Embodiment 2

[0066] In Vitro Release of Multifunctional Magnetic Nanoprobes Loaded with Methylene Blue

[0067] Put 5mL of the aqueous solution of the multifunctional magnetic nanoprobe into a dialysis bag with a molecular weight cut-off of 8000-14000, then put the dialysis bag into a beaker containing 100mL of PBS solution with pH=7.40.02M, and seal the beaker with plastic wrap . Place the beaker in a shaker and set the shaker speed to 100 rpm. Drug release experiments were performed at 37°C. Remove 2 mL of release medium every 0.5 h or 1 h and quickly add 2 mL of fresh media. After 72 hours, the concentration of MB in the release medium was measured using a UV-Vis spectrophotometer at 664 nm. The released drug amount was converted according to the standard curve, and the drug release rate was calculated. The curve obtained from (attached Figure 8 ) shows that the drug release rate is faster in the first 12 hours, and most of the drug release behavior occurs in this stage. After mo...

Embodiment 3

[0069] Cytotoxicity Evaluation of Multifunctional Magnetic Nano(Particle) Probes

[0070]NIH3T3 cells (mouse embryonic fibroblasts) and MCF-7 cells (human breast cancer cells) were used to evaluate the cytotoxicity of free methylene blue compounds and multifunctional magnetic nanoprobes by MTT (thiazolium blue) method. The experimental results are attached Figure 9 As shown, it can be seen that with the increase of MB concentration, the cell survival rate decreases, and the lowest reaches about 15%, indicating that free MB is more toxic to cells and is not suitable for direct use as drugs; while MB-loaded magnetic nanoparticles The toxicity is relatively small, and the cell survival rate is above 85% after 24 hours of action with cells, and the cell survival rate is still higher than 82% when the action time is extended to 48 hours, and has little dark toxicity to cells. This result shows that MB loading to Fe 3 o 4 mSiO 2 -FA magnetic nanoparticles can greatly reduce the...

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Abstract

The invention relates to a multifunctional magnetic nano particle and a preparation method thereof. The multifunctional magnetic nano particle has a core-shell structure, wherein the core is a medical magnetic nano particle, and the shell is photodynamic drug loaded nano silica with a mesoporous structure, hydrophilic groups and a targeting reagent which are safe to human bodies or animals are also modified on the shell. The specific surface area (BET) of the multifunctional magnetic nano particle is 600m<2> / g-800m<2> / g. The multifunctional magnetic nano particle has good controllable functions, and integrates functions of tumor targeting, imaging and photodynamic therapy.

Description

technical field [0001] The invention relates to a novel multifunctional magnetic nano particle integrating tumor targeting, imaging and photodynamic therapy and a preparation method thereof. Background technique [0002] Human health is an eternal subject. With the development of human economy and society, people pay more and more attention to health issues. Currently, cancer is one of the most serious diseases threatening human health, and its incidence continues to rise, causing serious social and economic burdens. Due to the lack of high-sensitivity diagnosis and low-toxicity treatment methods, the early diagnosis and treatment of cancer is still one of the difficult problems in today's medicine. [0003] Nanotechnology has the potential to make great contributions in the fields of cancer prevention, diagnosis, imaging and treatment. The rapid development of nanobiomedicine also provides new ideas for the development of safe, efficient, specific and intelligent nanoprob...

Claims

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Application Information

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IPC IPC(8): A61K49/08A61K49/00A61K47/04A61K47/34A61K41/00A61P35/00
Inventor 蓝闽波赵雪伶赵红莉袁慧慧陈宗艳张登浩陶亮
Owner EAST CHINA UNIV OF SCI & TECH
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