Application of coumarone quinoline compound as difunctional pH probe

A benzofuran quinoline and compound technology, applied in the field of chemistry, can solve the problems of poor selectivity, low sensitivity, easy to be interfered by metal ions, etc., and achieves the effects of high selectivity, small buffer solution interference and stable structure

Active Publication Date: 2014-02-12
INT HEALTHCARE INNOVATION INST JIANGMEN
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Problems solved by technology

[0003] In the prior art, the ubiquitous problem of pH fluorescent probes is that although they have a wide response range to hydrogen ions, up to 8 pH units, they are usually only used in the detection of phy

Method used

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  • Application of coumarone quinoline compound as difunctional pH probe
  • Application of coumarone quinoline compound as difunctional pH probe
  • Application of coumarone quinoline compound as difunctional pH probe

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Embodiment 1

[0031] Fluorescent recognition of probes for pH

[0032] Prepare buffer solutions with different pH values ​​(pH 7.0, 6.0, 5.0, 4.6, 4.0, 3.6, 2.6, 2.2) containing probes, and the concentration of probes is 5×10 -6 mol / L. Using 391 nm as the excitation wavelength, the fluorescence spectra of solutions with different pH values ​​were measured. The experimental results are as follows: figure 1 shown. The intensity of the fluorescence emission peak of the probe buffer solution at 456 nm at each pH value is plotted against the pH, as shown in figure 2 shown.

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Abstract

The invention discloses application of a coumarone quinoline compound as a difunctional pH probe, and particularly relates to the application of coumarone quinoline compound as the probe in measuring pH value of a solution and detecting sour milk. The probe is simple to prepare and easy to obtain, has a stable structure, and is used for rapidly detecting pH value of the solution through a fluorescence spectrophotometer or ultraviolet and visible absorption spectrum or visual inspection. The method for detecting pH by the probe has the advantages of high sensitivity and good selectivity, rapidness, and visual inspection, can be used for detecting the pH value in strong acid environment, and has wide application prospect in environment monitoring, disease diagnosis, industrial production, and pollution discharge inspection.

Description

technical field [0001] The invention belongs to the field of chemistry and relates to the application of a benzofuran quinoline compound as a bifunctional pH probe. Background technique [0002] The conduct or completion of chemical reactions and many important physiological processes in organisms are closely related to pH. Therefore, accurate measurement of pH value is very important for chemical and biological research. The most commonly used method for measuring pH is the glass electrode method. Due to the disadvantages of electrochemical interference, metal ion interference, easy mechanical damage, and cell damage, the glass electrode method has defects such as large errors in the determination of strong acids and alkalis, and is not suitable for pH detection in vivo. Fluorescence spectroscopy is also used in the determination of pH value. It is a detection method based on optical signals. It has the advantages of high sensitivity, good selectivity, and real-time in-s...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 张焜付成杰卢宇靖邓强王郑亚胡冬萍周小芬杜志云郑希黄宝华方岩雄
Owner INT HEALTHCARE INNOVATION INST JIANGMEN
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