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Detection method of polystictus versicolor and preparation containing polystictus versicolor

A detection method and preparation technology, applied in the field of detection of versicolor versicolor and preparations containing versicolor versicolor, can solve the problems of few quality control methods, irrationality, and inability to reflect internal quality changes, and achieve high accuracy and novelty, Strong specificity and innovative effect

Active Publication Date: 2014-02-26
培力(南宁)药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Domestic versicolor products also use high performance gel chromatography to measure the molecular weight and distribution of polysaccharides to evaluate the quality of such products. This method can control the quality of products to a certain extent, but for products mainly containing polysaccharides There are few quality control methods for versicolor versicolor medicinal materials and their preparations. Because the effective polysaccharides they contain are a large class, it is difficult to evaluate the authenticity and pros and cons of the quality of medicinal materials and their preparations based on their polysaccharide content and molecular weight distribution range. The evaluation methods cannot reflect the changes in their overall internal quality, which is irrational and unscientific to a certain extent, and the specificity is also poor

Method used

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  • Detection method of polystictus versicolor and preparation containing polystictus versicolor
  • Detection method of polystictus versicolor and preparation containing polystictus versicolor
  • Detection method of polystictus versicolor and preparation containing polystictus versicolor

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Experimental program
Comparison scheme
Effect test

experiment example 1

[0030] Experimental example 1: Study on the characteristic map of versicolor versicolor medicinal material and its preparation

[0031] Since Yunzhi polysaccharides are a large class of heteropolysaccharides with complex structures, there is still no specific quality control method to distinguish different types of polysaccharides. However, the method of the present invention is stable, has good reproducibility, strong specificity, can distinguish different types of polysaccharides, and can be used to control and evaluate the quality of versicolor versicolor medicinal material and its preparations. The test results are as follows:

[0032] 1. Preparation of reference solution

[0033] Preparation of reference solution Accurately weigh an appropriate amount of standard monosaccharide, and dissolve it in water to prepare a suitable monosaccharide standard liquid.

[0034]Derivatization amount of reference solution Take an appropriate amount of the above reference solution, ...

experiment example 2

[0068] Experimental example 2: Determination of the content of each monosaccharide in Yunzhi medicinal materials and its preparations

[0069] Since the polysaccharide content of Versicolor versicolor medicinal material and its preparations is mostly measured by spectrophotometry and titration, and glucose is used as a reference substance, this type of method has poor specificity, low accuracy, and poor quality controllability. The present invention adopts its main single method The content of sugar components controls the quality of polysaccharides in traditional Chinese medicine. The test results show that the separation effect is good under the selected conditions, and the linearity, stability, repeatability, and recovery of each monosaccharide meet the requirements of content determination. It can be used to control the quality of polysaccharides in traditional Chinese medicine, and can effectively evaluate the quality of medicinal materials and preparations containing poly...

Embodiment 1

[0098] Example 1 Determination of monosaccharides in polysaccharides of Yunzhi extract preparations by ultra-high performance liquid chromatography

[0099]1. Determination of characteristic spectrum of Versicolor versicolor extract preparation by ultra-high performance liquid chromatography: according to high-performance liquid chromatography (Appendix VI D of Chinese Pharmacopoeia 2010 Edition);

[0100] Chromatographic conditions Instrument: watersUPLC, DAD detector; column: octadecylsilane bonded silica gel column (Kinetex C 18 100A, 4.6×150mm, 2.6μm), with acetonitrile and phosphate (weigh K 2 HPO 4 Add 800mL of water to dissolve 4.4g, add phosphoric acid to adjust the pH to 6.5, and add water to dilute to 1000mL) as the mobile phase for gradient elution as shown in the table below; column temperature: 35°C; detection wavelength: 245nm; injection volume: 2.0μl; flow rate : 0.9ml / min.

[0101]

[0102]

[0103] Preparation of reference solution Accurately weigh an...

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Abstract

The invention discloses a detection method of polystictus versicolor and a preparation containing polystictus versicolor. According to the method, monosaccharide fragments obtained after polysaccharides of polystictus versicolor and the preparation containing polystictus versicolor are hydrolyzed are detected by adopting ultra performance liquid chromatography or high performance liquid chromatography while mannitose, glucose, galactose, fucose and other monosaccharide fragments are taken as evaluating indicators, and a quality control and evaluation method for measuring characteristic spectra and content of the monosaccharide fragments is established. The method can be used for quality control and evaluation of a polystictus versicolor crude drug and a preparation containing the polystictus versicolor crude drug, a polystictus versicolor extract or a polystictus versicolor effective part. The method is used for evaluating the quality of the polystictus versicolor crude drug and the preparation by controlling the monosaccharide fragments obtained after polysaccharide of the polystictus versicolor and the preparation containing polystictus versicolor are hydrolyzed. The method is strong in specificity, high in accuracy, easy and simple to operate, easy to control and higher in practical value, and can be used for effectively controlling the product quality.

Description

technical field [0001] The invention relates to a detection method of versicolor versicolor and a preparation containing versicolor versicolor, in particular to a quality control and evaluation method of versicolor versicolor medicinal material and preparations containing versicolor versicolor, or versicolor effective parts, or versicolor effective parts group. Background technique [0002] Versicolor versicolor is the dry fruiting body of Coriolus versicolor (L.ex Fr.) Quel, a polyporaceae fungus. In the Eastern Han Dynasty, "Shen Nong's Materia Medica" has recorded that versicolor versicolor is used to treat cancer, and "Compendium of Materia Medica" records that versicolor versicolor has the effects of calming the nerves, prolonging life, sharpening joints, strengthening muscles and bones, and curing deafness. Modern studies have shown that Yunzhi polysaccharide extracted from Yunzhi can significantly improve the quality of life of cancer patients, enhance immunity, reduc...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 韦红言温庆伟周永强覃艳霞宁平行
Owner 培力(南宁)药业有限公司
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