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Composite magnetic nanoparticles for targeted therapy of liver cancer and preparation method thereof

A magnetic nanoparticle and composite magnetic technology, applied in the field of genetic engineering, can solve the problems of limited number of genes carried, single function of the carrier, and insufficient safety, etc., to achieve a large number of carried genes, good biocompatibility, safety and controllability sex-enhancing effect

Inactive Publication Date: 2014-03-05
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Viral vectors have high transfer efficiency, but they are not safe enough (such as immunogenicity, possible mutation of host genes, etc.), the amount of genes carried is limited, and the vector function is single.

Method used

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  • Composite magnetic nanoparticles for targeted therapy of liver cancer and preparation method thereof
  • Composite magnetic nanoparticles for targeted therapy of liver cancer and preparation method thereof
  • Composite magnetic nanoparticles for targeted therapy of liver cancer and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Preparation of Composite Magnetic Nanoparticles

[0024] (1) Preparation of plasmid p[HRE]AFP-p53: p[HRE]AFP-p53 is the name of a recombinant eukaryotic expression plasmid, where HRE refers to the hypoxia response sequence element, which enhances the expression of the plasmid AFP refers to the alpha-fetoprotein promoter, which mediates the downstream tumor suppressor gene p53 (the name of a gene) that can only be expressed in liver cancer cells. The alpha-fetoprotein promoter sequence was prepared by base synthesis, and the two ends of the synthesized sequence contained plasmid restriction endonuclease MluI (MluI is the name of the endonuclease, the same below) and HindIII sites, respectively, and the eukaryotic expression plasmid pCDNA3.1 (pCDNA3.1 is the name of a commonly used gene cloning plasmid, which was first synthesized and applied by Western Biological Company, and there is no corresponding Chinese name at present) and alpha-fetoprotein promoter fragment wi...

Embodiment 2

[0040] The basic steps and process are the same as in Example 1, except that when preparing composite magnetic nanoparticles: the ferroferric oxide magnetic nanoparticles modified with plasmid p[HRE]AFP-p53 and polyethyleneimine are respectively used in serum-free medium Dilute, and then mix the two evenly according to the mass ratio of magnetic nanoparticles to p[HRE]AFP-p53 of 2:1, and obtain composite magnetic nanoparticles for targeted treatment of liver cancer after incubation.

[0041] All the other implementation parts are the same as in Example 1.

Embodiment 3

[0043] The basic steps and process are the same as in Example 1, except that when preparing composite magnetic nanoparticles: the ferroferric oxide magnetic nanoparticles modified with plasmid p[HRE]AFP-p53 and polyethyleneimine are respectively used in serum-free medium Dilute, and then mix the two evenly according to the mass ratio of magnetic nanoparticles to p[HRE]AFP-p53 of 16:1, and obtain the composite magnetic nanoparticles for targeted treatment of liver cancer after incubation.

[0044] All the other implementation parts are the same as in Example 1.

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Abstract

The invention relates to composite magnetic nanoparticles for targeted therapy of liver cancer and a preparation method thereof. The method comprises the following steps: respectively preparing recombinant eukaryon expression plasmid p[HRE]AFP-p53 and polyethyleneimine-modified ferroferric oxide magnetic nanoparticles, evenly mixing the recombinant eukaryon expression plasmid p[HRE]AFP-p53 and polyethyleneimine-modified ferroferric oxide magnetic nanoparticles in a mass ratio of 2:1-16:1, and incubating for 30 minutes to obtain the p[HRE]AFP-p53 / polyethyleneimine ferroferric oxide magnetic nanoparticle compound. The compound prepared by the method is used for combination of targeted gene therapy of liver cancer and magnetic fluid thermotherapy, and has specific therapeutic effect for liver cancer in in-vivo and in-vitro therapeutic tests.

Description

technical field [0001] The invention belongs to the field of genetic engineering, and in particular relates to a composite magnetic nanoparticle for targeted treatment of liver cancer and a preparation method thereof. Background technique [0002] Hepatocellular carcinoma (HCC) is a common malignant tumor with an annual incidence of 5.5-14.9 per 100,000 people worldwide, and 600,000-1 million deaths from hepatocellular carcinoma. China has the highest incidence of hepatocellular carcinoma In my country, the annual incidence rate exceeds 30.3 / 100,000 people. Hepatocellular carcinoma is highly invasive and insidious. Most patients (80%) cannot be surgically removed and can only rely on chemotherapy, radiotherapy and other treatment methods. However, hepatocellular carcinoma is resistant to many traditional chemotherapeutic drugs, and liver radiation The tolerated dose is low and the therapeutic effect is limited. Therefore, people are gradually turning more attention to the f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00A61K49/06A61K47/02A61K9/14B82Y5/00A61P35/00
Inventor 张东生袁晨燕张皓
Owner SOUTHEAST UNIV
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