Composite culture medium and method for quickly detecting total bacterial count by using same

A culture medium and percentage technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of high price of rapid detection test paper, unsuitable for general application, long time consumption, etc. The effect of low, large colony morphology

Active Publication Date: 2014-03-05
YANTAI XIWANG FOOD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the detection reagent for the total number of colonies is usually plate counting agar, and most bacteria can form colonies that can be distinguished by the naked eye after 48 hours of cultivation on the agar, which takes a long time; the rapid detectio

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Accurately weigh 4.0g of tryptone, 6.0g of agar powder, 2.0g of beef extract, 2.0g of yeast extract, 2.0g of glucose, 0.2g of lecithin, 0.2g of complex vitamin B, and 608.0g of Tween. Medium.

Embodiment 2

[0028] Accurately weigh 10.0g of tryptone, 15.0g of agar powder, 5.0g of beef extract, 5.0g of yeast extract, 5.0g of glucose, 0.5g of lecithin, 0.5g of complex vitamin B, and 20.0g of Tween 6020.0g. Medium.

[0029] The method for rapidly detecting the total number of bacterial colonies of the above-mentioned compound culture medium comprises the following steps: adding 24.4 g of the compound culture medium of the above-mentioned embodiment 1 to 400 ml of distilled water, heating and boiling until completely dissolved, then adjusting the pH value to 7.0 to 7.5, and high-pressure Sterilize (115°C, 15min) for later use; then weigh 25±0.1g sample, add it to a sterile homogeneous bottle, add sterile diluent to 250g, and homogenize for 1min-2min to make the sample well dispersed. The ratio is 1:10; take 1.0mL of the above dilution, put them into 2 sterilized plates respectively, pour out the compound culture medium with the total number of colonies, culture in an incubator at 36°C...

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Abstract

The invention relates to a composite culture medium and a method for quickly detecting total bacterial count by using the same, belonging to the field of detection methods of total bacterial count in foods. The composite culture medium is prepared by mixing the following raw materials in percentage by weight: 10-20% of tryptone, 10-30% of agar powder, 1-10% of beef extract, 1-10% of yeast extract, 1-10% of glucose, 0.1-0.9% of lecithin, 0.1-0.9% of vitamin B complex and 10-40% of Tween 60. Compared with the prior art, the composite culture medium and method have the following advantages: the composite culture medium for quickly detecting total bacterial count can quickly detect the total bacterial count in various foods, the detectable rate is 20% higher than that of the commercially available culture medium (plate count agar), the colonial morphology is larger, and the price is lower than that of the commercially available total bacterial count detection test paper; and thus, the composite culture medium and method are suitable for wide-range popularization and use.

Description

technical field [0001] The invention relates to a compound culture medium and a method for rapidly detecting the total number of bacterial colonies, belonging to the field of detection methods for the total number of bacterial colonies in food. Background technique [0002] The total number of colonies is the total number of microbial colonies formed in each g (ml) of the sample obtained after the food sample is cultured under certain conditions (such as culture medium, culture temperature and culture time, etc.). At present, the detection reagent for the total number of colonies is usually plate counting agar, and most bacteria can form colonies that can be distinguished by the naked eye after 48 hours of cultivation on the agar, which takes a long time; the rapid detection test paper for the total number of colonies can solve this problem, but rapid detection The test paper is expensive and not suitable for general application, and there is no standard for detecting the to...

Claims

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Application Information

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IPC IPC(8): C12Q1/06
Inventor 赵瑞连徐世明郭光平张建梅厉建军王光杰宋玉洁孙晓红
Owner YANTAI XIWANG FOOD
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