Wall breaking method for red yeast

A technology for red yeast and fermentation broth, applied in the biological field, can solve the problem of cell debris not being able to settle, and achieve the effects of strong development prospects, low concentration of hydrochloric acid, and low equipment requirements

Inactive Publication Date: 2014-03-19
ANHUI NORMAL UNIV
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  • Abstract
  • Description
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Problems solved by technology

The invention solves the technical problem that the broken cell fragments cannot be precipitated in the centrifugal system after being treated with high-con...

Method used

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  • Wall breaking method for red yeast
  • Wall breaking method for red yeast
  • Wall breaking method for red yeast

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Experimental program
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Embodiment Construction

[0053] A preferred embodiment of the various embodiments of the present invention.

[0054] 1. Preparation of hydrochloric acid with different concentration gradients

[0055] Take a certain amount of concentrated hydrochloric acid and add distilled water to dilute to the relevant concentration gradient. According to the relevant literature, the concentration gradient of hydrochloric acid is determined to be 1mol / L, 2mol / L, 3mol / L, 4mol / L, 5mol / L, and 6mol / L.

[0056] The above-mentioned hydrochloric acid reagent is analytically pure AR with a substance concentration of 12mol / L, produced by Shanghai Bohe Fine Chemicals Co., Ltd. All concentrations of hydrochloric acid must be prepared and used immediately.

[0057] 2. The steps of treating red yeast by acid heat method

[0058] (1) Fermentation culture of red yeast

[0059] The preserved Rhodotorula strains were transferred to PDA medium and activated at 28°C for 48h. Pick 2 rings and insert them into a 250mL Erlenmeyer fla...

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Abstract

The invention relates to a wall breaking method for red yeast. The wall breaking method comprises the following steps: preparing hydrochloric acid with different concentration gradients, performing treatment on the red yeast by an acid-heating method, extracting pigments by an organic solvent and comparing a wall breaking effect. The concentration of the hydrochloric acid used by the wall breaking method for the red yeast, provided by the invention, is low, the wall breaking time is short, the efficiency is high, and the extraction rate of carotenoids is improved by about 40% in comparison with the treatment with the acid in other concentrations; and furthermore, the energy consumption is low, the process is simple, the requirements on equipment are low, the pollution of the carotenoids is reduced by treatment with the hydrochloric acid in low concentration, and the method has very strong practical value and development prospect. The wall breaking method is conductive to improving the yield and the quality of the carotenoids produced by fermentation of the red yeast and reducing the production cost.

Description

technical field [0001] The invention relates to biotechnology, in particular to a method for breaking the red yeast cell wall, which uses an acid-heat method to break the red yeast cell wall, so as to achieve the purpose of extracting intracellular pigment. Background technique [0002] Carotenoids are natural pigments widely found in plants and microorganisms. In addition to the biological activity of converting into vitamin A, it also has various physiological functions such as preventing cancer and cardiovascular diseases. The carotenoids produced by Rhodotorula have the advantages of simple nutritional requirements, short growth cycle and rich nutrients in the bacteria, and have great practical value and development prospects. However, carotenoids are intracellular products of Rhodotorula cerevisiae, and the wall breaking effect and extraction method directly affect the yield, quality and production cost of carotenoids produced by Rhodotorula cerevisiae fermentation. T...

Claims

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Application Information

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IPC IPC(8): C12N1/06C12R1/645
Inventor 高娜马雪李瑞萍
Owner ANHUI NORMAL UNIV
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