Fingerprint spectrum detection method of compound donkey-hide gelatin preparation

Abstract

The invention discloses a fingerprint spectrum detection method of a traditional Chinese medicine compound donkey-hide gelatin preparation. The method comprises the chromatographic conditions: a chromatographic column with octadecylsilane chemically bonded silica as a filling agent is adopted; a mobile phase A is a sodium dihydrogen phosphate solution with the concentration of 0.01 mol / L-1 mol / L or a potassium dihydrogen phosphate solution with the concentration of 0.01 mol / L-1 mol / L or a formic acid solution with the concentration of 0.1%-5% or a phosphoric acid solution with the concentration of 0.02%-5%; B is acetonitrile or methanol; and gradient elution has the flow rate of 0.5-1.5 ml / min, the detection wavelength of 190-410 nm, and the column temperature of 25-55 DEG C. The method has relatively high precision, good stability and good reproducibility, and can be more comprehensively and effectively control the quality of the preparation.

Description

technical field [0001] The invention belongs to the technical field of medicine quality control, in particular, the invention relates to a fingerprint detection method of a compound donkey-hide gelatin preparation. Background technique [0002] Compound donkey-hide gelatin is a Chinese patent medicine for invigorating qi and nourishing blood, and it has been used in my country for many years. The preparation is made from the following raw materials, 5 parts of donkey-hide gelatin, 6 parts of red ginseng, 8 parts of rehmannia glutinosa, 12 parts of Codonopsis pilosula, and 5 parts of hawthorn. The preparation method of making the above-mentioned components into the medicine of the present invention is as follows: [0003] ① Weigh the raw Chinese medicinal materials according to the above dosage; [0004] ② Add appropriate amount of water to the above raw Chinese medicinal materials to extract, concentrate, filter, and set aside; [0005] ③ Add appropriate amount of water to...

AI Technical Summary

Problems solved by technology

[0008] At present, the detection of this preparation generally only uses Rg1, Re, Rb1, and total nitrogen as detection indicators, but it cannot fully reflect the quality of the product at all, and it is not very reasonable to control the quality of traditional Chinese medicine compound preparations only by this; if other The first is that the content is low, and the second is that there are no ready-made detection methods and detection conditions, so it is difficult to establish a suitable detection method

Images