A rapid detection kit for furaltadone metabolites by chemiluminescent ELISA

Abstract

The invention relates to a furaltadone metabolite chemiluminescent enzyme linked immune sorbent rapid analysis kit. The furaltadone metabolite chemiluminescent enzyme linked immune sorbent rapid analysis kit comprises a luminescence test plate, a furaltadone metabolite standard solution, a furaltadone metabolite monoclonal antibody working solution, an enzyme-labeled secondary antibody, a luminescence liquid, a sample diluent, and a washing concentrate. The furaltadone metabolite monoclonal antibody working solution is a solution of Balb-c mouse immune antibody, and the solution is diluted according to a volume ratio of 1:3500 for application, wherein a furaltadone metabolite-bovine serum albumin conjugate is taken as an immunogen of Balb-c mouse immune antibody; the enzyme-labeled secondary antibody is a goat anti mouse IgG working solution labeled by the furaltadone metabolite-horse radish peroxidase, and is diluted according to a volume ratio of 1:10000 for application; and the luminescence liquid comprises a luminescent substrate A, and a luminescent substrate B. The furaltadone metabolite chemiluminescent enzyme linked immune sorbent rapid analysis kit is high in sensitivity, wide in linear dynamic range, long in optical signal duration, rapid in analyzing, stable in results, less in error, high in safety, and long in usage period.

Description

technical field [0001] The invention belongs to the field of immunological detection, and mainly relates to a kit for rapid detection of furaltadone metabolite chemiluminescent ELISA. Background technique [0002] At present, there are mainly the following methods for the detection of furaltadone metabolite antibiotic residues in milk: [0003] (1) Microbial obstruction detection method, the detection method of my country's standard for the detection of antibiotic residues in fresh milk (GB4789.27-2003), the antibacterial zone test and turbidity test commonly used abroad in the 1970s and 1980s, and the current market sales The color-changing detection kits belong to this category. Different types of indicator bacteria have different sensitivities to different types of antibiotics. Selecting appropriate detection indicator bacteria can reduce the detection limit and improve the detection sensitivity. However, the microbiological detection operation is time-consuming and labo...

AI Technical Summary

Problems solved by technology

[0005] After the 1990s, the vast majority of physical and chemical detection methods for the determination of furaltadone metabolite drug residues in food mainly rely on liquid chromatography for separation, followed by chromatography / mass spectrometry, gas chromatography, and high-performance thin-layer chromatography. Because of their unique performances, detection methods such as detection methods have been slightly applied in the detection of antibiotic residues.

At present, enzyme-linked immunosorbent assay, radioimmunoluminescence assay, fluorescence immunoassay, and chemiluminescence are mainly used. Compared with luminescence, enzyme immunoassay has lower sensitivity.

Images