Production method of virus-free micro seed potato

A technology for miniature seed potatoes and production methods, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of low detoxification efficiency, poor quality of detoxified seedlings, etc., achieve good detoxification effect, and remove viruses. The effect of enhancing capacity and improving quality

Inactive Publication Date: 2014-03-26
HARBIN RUIZHE TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims to solve the technical problems of low detoxification efficiency and poor quality of detoxified seedlings existing in the existing potato detoxification method for producing miniature seed potatoes; and provides a production method for detoxification miniature seed potatoes

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0013] Specific embodiment one: the production method of potato detoxification miniature seed potato is carried out according to the following steps in the present embodiment:

[0014] Step 1. Potato cubes produced by healthy and excellent plants with obvious variety characteristics (that is, skin color, flesh color, potato shape, bud eyes, etc., which meet the characteristic requirements) and without disease spots, insects, and mechanical trauma are used as detoxification materials. First break the dormancy of the potato pieces, then cover them with warm sand, place them in a dark condition at 25°C to accelerate germination, and when the stem tip is 1cm long, transfer them to a light incubator for high-temperature treatment for 20-25 days. High temperature treatment for 2 hours and then high temperature treatment at 38°C for 22 hours. On the seventh day of high temperature treatment, use 0.1~0.5 mg / L gibberellin (GA3) +0.1~0.5 mg / L6-benzylaminopterin (6BA) +0.1~0.5 mg / LD-calc...

specific Embodiment approach 2

[0018] Specific embodiment two: the difference between this embodiment and specific embodiment one is: in step one, soak the seeds with 15 thiourea+5 mg / L gibberellin for 5 minutes to break dormancy. Other steps and parameters are the same as in Embodiment 1;

specific Embodiment approach 3

[0019] Embodiment 3: This embodiment differs from Embodiment 1 or Embodiment 2 in that step 2 is soaked with 70% (mass concentration) ethanol. Other steps and parameters are the same as those in Embodiment 1 or 2;

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Abstract

The invention discloses a production method of virus-free micro seed potatoes, and belongs to the field of seed potato production. The method disclosed by the invention is used for solving the technical problems of low virus-free efficiency and bad quality of virus-free seedlings of an existing production method of the virus-free micro seed potatoes. The method comprises the following steps: (I) selecting a virus-free material, breaking the dormancy of the potatoes, covering the potatoes with warm and humid sand, and accelerating germination at a high temperature; (II) stripping the shoot tip, flushing with tap water, soaking with ethanol, immersing in a sodium hypochlorite solution, and flushing with sterile water; (III) cutting 1-2 shoot apical meristems with leaf primordium, and performing in-vitro culture; and when the seedling number reaches the detection quantity, detecting seven viruses PLRV, PVA, PVY, PVM, PVX, PVS and PSTV of the seedlings, wherein if the results are all negative, the seedling is a virus-free seedling; and (IV) expanding propagation, hardening the seedlings and transplanting into a greenhouse for nutrient and water management; and culturing till harvest to obtain the micro potatoes. The method disclosed by the invention is used for producing the seed potatoes.

Description

technical field [0001] The invention belongs to the field of seed potato production. Background technique [0002] Potato is the fourth largest food crop in the world. It has strong adaptability and long industrial chain. It is an important food crop and industrial raw material crop. [0003] The accumulation of invading viruses in the process of plant reproduction leads to the annual decline of potato growth, fewer and fewer potatoes are harvested, and even extinction. [0004] At present, potato detoxification is used to make miniature seed potatoes to prevent potato degeneration, but there are technical problems of low detoxification efficiency and poor quality of detoxification seedlings. Contents of the invention [0005] The invention aims to solve the technical problems of low detoxification efficiency and poor quality of detoxified seedlings existing in the existing potato detoxification method for preparing miniature seed potatoes; and provides a production metho...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 张引强
Owner HARBIN RUIZHE TECH DEV
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