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Indosasa hispida McClure 'rainbow' anthocyanin extract

A technology of anthocyanin extract and colorful red bamboo, applied in food science, azo dyes, organic dyes, etc., can solve problems such as easy deactivation, and achieve low-cost effects

Inactive Publication Date: 2015-03-25
YUNNAN ACAD OF FORESTRY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the high activity of anthocyanins, they are easily inactivated, and there is no pure anthocyanins on the market, so the extraction of highly stable anthocyanins is of great significance for the in-depth research and development of anthocyanins.

Method used

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  • Indosasa hispida McClure 'rainbow' anthocyanin extract
  • Indosasa hispida McClure 'rainbow' anthocyanin extract
  • Indosasa hispida McClure 'rainbow' anthocyanin extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) With 1 kg of colorful red bamboo, 1% HCl-ethanol solution is used as the extraction solution, the weight ratio of liquid to material is 20:1, and cold soaked in the dark for 48 hours; after that, fresh solvent is replaced to continue cold soaking, and the extraction is 2 times;

[0021] (2) The filtrates extracted twice were combined, filtered, concentrated under reduced pressure, and the temperature was controlled at 50°C, so that the relative density of the concentrated extract was 1.02;

[0022] (3) Dissolve the concentrate with distilled water, filter, adjust the pH value to 6 with NaOH, and place it;

[0023] (4) The filtrate is eluted with AB-8 type macroporous adsorption resin, 20% ethanol is the eluent, and 30% or 45% or 60% ethanol eluate is collected, concentrated under reduced pressure, and obtained after drying Anthocyanin Extract.

Embodiment 2

[0025] (1) With 1 kg of colorful red bamboo bamboo poles, 1% HCl-ethanol solution is used as the extraction solution, the liquid-to-material weight ratio is 30:1, and cold soaked in the dark for 48 hours, then replaced with a fresh solvent and continued cold soaked for 3 times;

[0026] (2) Merge and filter the 3 times of cold dipping liquids, concentrate under reduced pressure, and control the temperature at 40°C to obtain a concentrated extract with a relative density of 1.12;

[0027] (3) Dissolve the concentrate with distilled water, filter and adjust the pH value to 7 with NaOH, and place it;

[0028] (4) The filtrate is eluted with D-101 type macroporous adsorption resin, 40% ethanol is the eluent, and 20% or 35% or 50% ethanol eluate is collected, concentrated under reduced pressure, and obtained after drying. Anthocyanin Extract.

Embodiment 3

[0030] (1) With 1 kg of colorful red bamboo bamboo poles, 1% HCl-methanol solution is used as the extraction solution, the liquid-to-material weight ratio is 40:1, and cold soaked in the dark for 48 hours, and then replaced with a fresh solvent to continue cold soaking, a total of 4 cold soaks;

[0031] (2) Combine and filter the 4 times of cold soaking liquid, and concentrate under reduced pressure in a conventional manner. When the temperature is room temperature, the relative density is 1.20;

[0032] (3) Dissolve the concentrate with distilled water, filter, adjust the pH value to 8 with NaOH, and place it;

[0033] (4) The filtrate is eluted with D-3520 type macroporous adsorption resin, 40% ethanol is the eluent, and 40% or 60% or 80% ethanol eluate is collected, concentrated under reduced pressure, and obtained after drying Anthocyanin Extract.

[0034] Practical application shows that: the preparation process of colorful red bamboo anthocyanin extract only uses 1% HCl...

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Abstract

The invention discloses an indosasa hispida McClure 'rainbow' anthocyanin extract, and belongs to the technical field of natural product extracts. The extract is obtained by the following steps: (a), cutting indosasa hispida McClure 'rainbow' into the diameter of + / -1 cm, carrying out cold soaking for 48 hours in a dark place by taking 1 percent HCl-ethanol solution as an extraction solution and controlling the weight ratio of the solution to a material being 20-40:1, replacing a fresh solution, continuing performing cold soaking and extracting for 2 to 4 times in total; (b), merging the filtrates extracted for 2 to 4 times, and concentrating under reduced pressure conventionally, wherein the temperature is controlled to be room temperature to 50 DEG C, and the relative density of the obtained concentrated extract is 1.02 to 1.20; (c), dissolving the concentrate by using distilled water, filtering, adjusting the pH value to be 6 to 8 by using NaOH, and standing; (d), eluting by using 20 to 60 percent ethanol, loading the filtrate to macroporous resin, eluting, collecting 20 to 80 percent ethanol eluate, concentrating under reduced pressure conventionally, and dying to obtain the total anthocyanin extract of the indosasa hispida McClure 'rainbow'. The extract has the advantages of no toxicity, no pollution and low cost; the anthocyanin content of the indosasa hispida McClure 'rainbow' anthocyanin extract reaches over 30 percent. Theoretical foundations are laid for the development and the stabilization of anthocyanin food coloring matters, anthocyanin functional food, and health products.

Description

Technical field: [0001] The invention relates to a colorful red bamboo anthocyanin extract, which belongs to the technical field of natural product extracts. Background technique: [0002] Colorful red bamboo (Indosasa hispida McClure "rainbow") is a plant of the genus Indosasa in the subfamily Bambusoideae of the family Poaceae. The culm is 2-3m high and 1-3cm in diameter. There are small bristles on the surface when it is young, and then it falls off and becomes glabrous, with white powder below the nodes; there are more than 3 branches in each node in the middle of the culm, and the branches are erect, and the middle and lower parts of the bamboo culm are different. Degree of red to purple. Leaf blade presents light yellow stripes, band-like lanceolate or lanceolate, outer length 9-23cm, width 1.5-4cm, apex long and acuminate, base cuneate or wide cuneate, lower surface often pubescent, rarely glabrous , with small serrations on both edges, 5-6 pairs of secondary veins,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L1/29C09B61/00A23L33/00
CPCA23L1/3002C09B61/00A23L1/2751A23L5/43A23L33/105
Inventor 杨宇明施蕊王娟张兴波周远
Owner YUNNAN ACAD OF FORESTRY