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A kind of extraction method of lactone in ginkgo ginkgo

An extraction method and ginkgo technology, applied in the extraction of ginkgo ginkgo active components and the extraction of lactones in ginkgo ginkgo, can solve the problems of complicated operation, low yield, low purity, etc., and achieve high purity, high product yield, good extraction effect

Active Publication Date: 2015-12-09
南通市通州区五接农业机电管理站有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods all have the problems of complicated operation, low yield and low purity.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Get the liquid liquid;

[0022] Step 2, add 30kg of isot butanol to the liquid liquid, mix well, absorb resin through large holes for removing miscellaneous, collect through the liquid;

[0023] Step 3, 1%sodium hydroxide solution with a mass concentration of 1%hydroxide solution, through the pH of the liquid to 8.0, and then filter it with ultrafiltration membrane to obtain ultrafiltration through the liquid;

[0024] Step 4, add 50kg of ethyl acetate to the ultrafiltration through the liquid to extract the organic phase;

[0025] Step 5, heated the organic phase to be heated and concentrated. When the volume of the material is reduced to 30%, the crystals are cooled, and the crystals are filtered out and dried.

[0026] The large hole adsorption resin in Step 2 is HP20 polystyrene pores adsorption resin. The intercept molecular weight used in step 3 is 200,000 dada, and the drying temperature in step 5 is 80 ° C.The dry time is 4 hours.

[0027] A total of 30g of ginkgo l...

Embodiment 2

[0029] Step 1, take 40kg of ginkgo, dry it at 120 ° C for 6 hours, grind it into powder, add to 200kg of potassium sulfate solution with 1%, stir well, heat to 60 ° C, heat up for 4 hours, cool down, cool down, cool, cool, cool, cool, cool, cool, cool, cool, cool, cool, cool, and let it cool.Get the liquid liquid;

[0030] Step 2, add 40kg of isot butanol to the liquid liquid, mix well, absorb resin through large holes for removing miscellaneous, collect through the liquid;

[0031] Step 3, 1%sodium hydroxide solution with a mass concentration of 1%hydroxide solution, through the pH of the liquid to 10.0, and then filter it with ultrafiltration membrane to obtain ultrafiltration through the liquid;

[0032] Step 4, add 60kg of ethyl acetate to the ultrafiltration through the liquid to extract the organic phase;

[0033] Step 5. Heat and concentration of organic phase. When the volume of the material is reduced to 50%of the original, the crystals are cooled, and the crystals are fi...

Embodiment 3

[0037] Step 1, take 35kg of ginkgo, dry it at 110 ° C for 5 hours, grind it into powder, add to 170kg of potassium sulfate solution of 0.7%, stir well, heat it to 55 ° C, heat for 3 hours, let it cool, let it cool, let it cool, let it cool, let it cool down, cool, cool, and let it cool.Get the liquid liquid;

[0038] Step 2, add 35kg of isot butanol to the liquid liquid, mix well, absorb resin through large holes for removing miscellaneous, collect through the liquid;

[0039] Step 3, a sodium hydroxide solution with a mass concentration of 1%hydroxide solution adjusts through the pH of the liquid to 9.0, and then filter it with an ultrafiltration membrane to obtain the ultrafiltration through the liquid;

[0040] Step 4, add 55kg of ethyl acetate to the ultrafiltration through the liquid to extract the organic phase;

[0041] Step 5, heated the organic phase to be heated and concentrated. When the volume of the material is reduced to 40%, the crystals are cooled, and the crystals...

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PUM

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Abstract

The invention relates to a method for extracting active ingredients from ginkgo nuts, in particular to a method for extracting lactone from the ginkgo nuts, and belongs to the technical field of natural product extraction. The method comprises the following steps: taking and drying ginkgo nuts, grinding the ginkgo nuts into powder, adding the powder in a potassium sulfate solution, mixing the solution uniformly, heating, preserving heat and cooling to obtain a leach liquor; adding isobutanol in the leach liquor, mixing the liquor uniformly, removing impurities through macroporous resin, and collecting the permeating liquid; adjusting the pH value of the permeating liquid, filtering with an ultrafiltration membrane to obtain an ultrafiltration permeating liquid; adding ethyl acetate in the ultrafiltration permeating liquid for extraction to obtain the organic phase; heating and concentrating the organic phase, cooling, crystallizing, and filtering out crystals, drying the crystals to obtain the final product. Through the adoption of the method for extracting lactone from ginkgo nuts, impurities can be relatively well removed from the leach liquor, the active ingredients can be enabled to be extracted better, and the product is high in yield and purity.

Description

Technical field [0001] The present invention involves a method of extraction of a ginkgo white fruit active ingredient, especially the extraction method of ginkgo ginkgo, which is a natural product extraction technology field. Background technique [0002] Ginkgo is also known as Ginkgo and Gongsun Shizi.It is like a white and white as white as jade, which is sweet, bitter, and astringent.Glateophenols, five carbon polysaccharides, lipidosterol and other ingredients.It has the effects and medical effects such as nourishing lung qi, cure cough, stopping insects, narrowing, flat wrinkles, protective blood vessels, and increased blood flow. [0003] In the 1980s, studies found that ginkgo lactatophyte was a strong antagonist of platelet activation factor (PAF). It had significant curative effects on cardiovascular and cerebrovascular diseases and caused a climax of international ginkgo research.ongoing.Ginkgo strain is a dizomantic compound.At present, there are 8 dietth esters sepa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D493/20
CPCC07D493/22
Inventor 袁宏亮
Owner 南通市通州区五接农业机电管理站有限公司