Preparation method of armillaria concentrate

Abstract

A preparation method of armillaria concentrate comprises the following steps: inoculating a bacterial strain onto a sterile culture medium, and culturing the bacterial strain for 3-4 days at 27 degrees plus or minus 1 degree to obtain mycelium; in terms of parts by weight, taking 3-5 parts of glucose, 1-3 parts of peptone, 1-3 parts of yeast extract, 0.1-0.5 part of KH2PO4, 0.1-0.5 part of MgSO4, 0.001-0.003 part of NaCl and 0.05-0.1 part of vitamin B1, adding 100-150 parts of purified water, and uniformly mixing to obtain a culture solution; inoculating the mycelium into the culture solution, and fermenting for 3-5 days under the conditions that the temperature is 27 degrees plus or minus 1 degree, the rotating speed is 100-500 rpm, the ventilation amount is 100-150 L / h, and the pH value is 5.0; collecting fermentation liquid, filtering the fermentation liquid, and concentrating the fermentation liquid at reduced pressure till the density (at 20 DEG C) is 1.030-1.035 to obtain the armillaria concentrate. The preparation method of the armillaria concentrate has the advantages as follows: the composition of the culture medium is clarified, so that production operation is facilitated; the production cycle is shortened; the fermentation liquid is relatively high in yield; the adenosine content of the fermentation liquid is higher than that in the original production technology.

Description

Technical field: [0001] The invention belongs to the field of pharmacy, and in particular relates to a method for preparing a concentrated liquid of Armillaria armillaria, a raw material medicine of traditional Chinese medicine. Background technique: [0002] Armillaria mellea (Vahl ex Fr.) Quel is a medicinal and edible fungus belonging to Trichol omataceae (Trichol omataceae) Armillaria (Fr.) Staude. ) symbiotic famous medicinal fungi. After many years of research at home and abroad, various active ingredients such as Armillaria polysaccharides, sesquiterpenoids and purines have been isolated. Armillaria fruiting bodies have hypnotic, sedative, anti-vertigo, improved heart and brain blood circulation and strong brain protection, can treat a variety of diseases, and can improve the immune function of the body. Armillaria Armillaria is one of the nourishing and health-care traditional Chinese medicinal materials in my country, and is favored by scholars at home and abroad....

AI Technical Summary

Problems solved by technology

However, due to the unclear composition of the culture medium of the Armillaria concentrated solution in the statutory standards, the existing preparation methods of the Armillaria concentrated solution have shortcomings such as long cultivation period and uncertain adenosine content, which affect large-scale production