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Method for preparing collagen peptide by hydrolyzing bovine bones, and collagen peptide

A collagen peptide and bovine bone technology, which is applied in the preparation methods of peptides, chemical instruments and methods, animal/human proteins, etc., can solve the problems of difficult industrial production, expensive ultra-high pressure microfluidic equipment, etc., and achieve enzymatic hydrolysis time. Short, good dispersibility, good enzymatic hydrolysis effect

Pending Publication Date: 2020-07-03
PINGLIANG HUAKE BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the high-frequency ultrasonic equipment and ultra-high pressure micro-fluidic equipment used in this patent are expensive, and it is difficult to achieve large-scale industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A method for hydrolyzing bovine bone to prepare collagen peptides, comprising the following steps:

[0028] S1: Take bovine bones, clean them, and freeze them at -20~-15°C for 2.5 hours, then freeze them at -10~-5°C for 4.5 hours, and finally freeze them at -4~-1°C for 2.5 hours.

[0029] S2: Grinding and pulverizing the frozen-preserved bovine bone in step S1 into bone dregs; the particle size of the bone dregs in the step S2 is 5000 mesh.

[0030] S3: Add 1 times the water to the bone residue described in step S2, homogenize, adjust the temperature to 40-50°C, then add protease, and perform enzymolysis under certain conditions; the condition for enzymolysis is: pH is 6-8 , the reaction temperature is 65° C., and the reaction time is 40 minutes; the protease is a neutral protease.

[0031] S4: After the enzymolysis is completed, the enzyme is deactivated, filtered through a plate and frame filter press, dried after double-effect concentration, and the bovine bone coll...

Embodiment 2

[0034] A method for hydrolyzing bovine bone to prepare collagen peptides, comprising the following steps:

[0035] S1: The bovine bone was cleaned and stored at -20°C for 2 hours, then at -10°C for 4 hours, and finally at -4°C for 2 hours.

[0036] S2: Grinding and pulverizing the frozen-preserved bovine bone in step S1 into bone dregs; the particle size of the bone dregs in the step S2 is 6000 mesh.

[0037] S3: Add 1.5 times of water to the bone dross described in step S2, homogenize, adjust the temperature to 40-50°C, then add protease, and perform enzymolysis under certain conditions; the conditions for enzymolysis are: pH 6-8 , the reaction temperature is 55-75° C., and the reaction time is 35 minutes; the protease is a neutral protease.

[0038] S4: After the enzymolysis is completed, the enzyme is inactivated, purified and refined by membrane separation, dried after double-effect concentration, and the bovine bone collagen peptide is obtained. The falling film double-...

Embodiment 3

[0041] A method for hydrolyzing bovine bone to prepare collagen peptides, comprising the following steps:

[0042] S1: Bovine bones were cleaned and stored at -1°C for 11 hours; first at -15°C for 3 hours, then at -5°C for 5 hours, and finally at -1°C for 3 hours.

[0043] S2: Grinding and pulverizing the frozen-preserved bovine bone in step S1 into bone dregs; the particle size of the bone dregs in the step S2 is 6000 mesh.

[0044] S3: Add 0.5 times of water to the bone dregs described in step S2, homogenize, adjust the temperature to 40-50°C, then add protease, and perform enzymolysis under certain conditions; the conditions for enzymolysis are: pH 6-8 , the reaction temperature is 55-75° C., and the reaction time is 45 minutes; the protease complex protease from Novozymes.

[0045] S4: After the enzymolysis is completed, the enzyme is deactivated, filtered through a plate and frame filter press, dried after double-effect concentration, and the bovine bone collagen peptide...

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Abstract

The invention discloses a method for preparing collagen peptide by hydrolyzing bovine bones, and the collagen peptide. The method comprises the following steps: S1, cleaning bovine bones, and freezingand preserving the cleaned bovine bones at a temperature from -20 DEG C to -1 DEG C for 3-11 hours; S2, grinding and crushing the frozen and preserved bovine bones in step S1 into bone residues; S3,adding 0.5-1.5 times of water into the bone residues in step S2, performing homogenizing, adjusting the temperature to 40-50 DEG C, then adding protease, and performing enzymolysis under a certain condition; and S4, performing enzyme deactivation treatment after the enzymolysis is finished, performing filtering by a plate-frame filter press or performing purifying refining by membrane separation,and performing drying after double-effect concentration to obtain the bovine bone collagen peptide. The technological method provided by the invention has the advantages of short enzymolysis time andgood enzymolysis effect; and the obtained protein peptide product is easy to dissolve in water, and has a high nutritional value and good dispersibility.

Description

technical field [0001] The invention relates to the field of preparation of protein peptides, in particular to a method for preparing collagen peptides by hydrolyzing bovine bone and the collagen peptides. Background technique [0002] Collagen is a structural protein of the extracellular matrix, the main component of animal connective tissue, and the most abundant and widely distributed functional protein in mammals. It is involved in the formation, maturation, transmission of information between cells, and joint Lubrication, wound healing, calcification, blood clotting and aging are closely related. In addition to the many physiological activities of collagen, collagen peptides also have properties that collagen does not have. Compared with collagen, collagen peptide, as a small molecule peptide, has a lower molecular weight and better digestion and absorption. It can be directly absorbed by the intestinal tract, enter the blood, and reach various parts of the body such a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34
CPCC12P21/06C07K14/78
Inventor 员斌
Owner PINGLIANG HUAKE BIOLOGICAL TECH
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