Microorganism needle-type breeding and culturing integrated method

Abstract

The invention relates to a microorganism needle-type breeding and culturing integrated method of which the technology belongs to the technical field of artificial culture of microorganisms. The microorganism needle-type breeding and culturing integrated method aims to solve the technical problem of integration of inoculation, sterilization and disinfection which are necessary in the culturing process of microorganism mother strains (such as preservation of the mother strains, expanding propagation of the mother strains and production of the mother strains). The technical scheme of the microorganism needle-type breeding and culturing integrated method has the key points that a needle hole stirrer is created to implement integration of the operation links, for example, solid mother strains cultured in an injector are smashed, so that the injector can directly carry out extraction and injection; a controllable electric heater, a flexible sealing device and the like are organized to carry out high-temperature variable-pressure exhaust sterilization and disinfection and environmental-friendly substances such as hydrogen peroxide and yellow wax to carry out cleaning; and the internal circulation of mother strain packaging, the injector, target packaging, a ventilation conduit and mother strain packaging under the condition that V liquid drainage =V gas exhaust =V gas return, so that the possibility that the microorganisms are in contact with external microorganisms is reduced. The microorganism needle-type breeding and culturing integrated method has the main application of being used for the operating process separately completed by one person so as to enable the microorganisms to be subjected to high-efficiency breeding, inoculation and culturing under the controllable condition of no contact with the microorganisms in the outside air.

Description

technical field [0001] Methods of artificial cultivation of microorganisms. Integrate the breeding, cultivation and inoculation links of microbial culture. Background technique [0002] Liquid microbial parent species are generally easier to inoculate than solid microbial parent species, and have advantages in terms of uniform dispersion and growth speed. The cultivation technology of liquid microbial parent species is more popular, but both solid and liquid microbial parent species require stirring and transfer. At the same time, there are problems in the current commonly used liquid microbial seed breeding technology, such as the long time required for shaking table seed production, which is likely to cause different bacterial ages and reduce the quality; There are many details of oxygen seed production and there are potential safety hazards. More importantly, since there are other links between the breeding and inoculation cultivation of the liquid microbial stock, thes...

AI Technical Summary

Problems solved by technology

It can be seen that there are problems such as the lack of integration of breeding and cultivation links in the existing technology, and it is not easy for non-professionals to master, which poses technical obstacles to the small-scale microbial experiments and edible fungus production required.

In short, there are links that can be integrated in the breeding and cultivation of the existing microbial artificial cultivation technology, but it is these links that are not easy to operate, increase pollution and cost, and are not conducive to improving the efficiency of artificial cultivation and increasing economic benefits.

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