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Application of salvianolic acid A in preparation of medicine for treating tumour multi-drug resistance

A multi-drug resistance, salvianolic acid technology, applied in anti-tumor drugs, drug combinations, pharmaceutical formulations, etc., can solve the problems of inability to be widely used in clinical practice, large toxic and side effects, and achieve good water solubility and good bioavailability. Effect

Active Publication Date: 2014-04-02
NANJING GENERAL HOSPITAL NANJING MILLITARY COMMAND P L A
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Aiming at the mechanism of multidrug resistance, many drug resistance reversal agents have been discovered continuously, but due to their high toxicity and side effects, they cannot be widely used in clinical practice

Method used

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  • Application of salvianolic acid A in preparation of medicine for treating tumour multi-drug resistance
  • Application of salvianolic acid A in preparation of medicine for treating tumour multi-drug resistance
  • Application of salvianolic acid A in preparation of medicine for treating tumour multi-drug resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The effect of salvianolic acid A on the proliferation of human breast cancer cell MCF-7 and drug-resistant cell MCF-7 / MDR was detected by MTT assay. Human breast cancer cells MCF-7 in the logarithmic growth phase and drug-resistant cells MCF-7 / MDR were seeded in 96 microwell culture plates with a cell number of 5×10 3 / well at 37°C, 5% CO 2 After culturing in the incubator for 12 hours, add drugs in groups, set up parallel wells for each concentration, add normal saline to the negative control, add MTT10μl (5mg / ml) to each well after 24 hours, and culture in the incubator for 4 hours, discard the upper Add 100 μl of DMSO to each well, shake until the precipitate is completely dissolved, and measure the optical density (OD) value of each well at 490 nm. Cytotoxicity MTT assay showed that salvianolic acid A had a dose-dependent inhibitory effect on the survival rate of breast cancer cell MCF-7 and its multidrug-resistant cell MCF-7 / MDR, and had a dose-dependent inhibitor...

Embodiment 2

[0023] The apoptosis of MCF-7 / MDR cells induced by salvianolic acid A was detected by flow cytometry. Inoculate MCF-7 / MDR cells in a 6-well plate, treat the cells with salvianolic acid A for 24 hours after 12 hours, discard the medium containing the drug in each well, wash 2 times with PBS, follow the instructions of the Annexin V-FITC kit The operation was detected by flow cytometry, and the results showed that salvianolic acid A could induce the apoptosis of drug-resistant tumor cells MCF-7 / MDR in a dose-dependent manner. see results figure 2 .

Embodiment 3

[0025] The changes of intracellular reactive oxygen species (reactive oxygen species, ROS) under the action of salvianolic acid A were detected by fluorescence spectrophotometry. Inoculate MCF-7 / MDR cells in a 24-well plate. After the cells adhere to the wall, treat the cells with salvianolic acid A for 24 hours. Discard the medium containing the drug in each well, wash it twice with PBS, and follow the requirements of the active oxygen detection kit. The step is to add reactive oxygen free radical fluorescent probe DCFH-DA, and detect the fluorescence intensity by fluorescence spectrophotometry. see results image 3 b.

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Abstract

The invention relates to the field of natural medicines, and discloses application of salvianolic acid A in preparation of a medicine for treating tumour multi-drug resistance. Compared with salvia extracts of tanshinones, the salvianolic acid A disclosed by the invention has good water solubility, and therefore, the salvianolic acid A has better bioavailability. According to the invention, the salvianolic acid A has obvious inhibiting effect to drug-resistant tumour cells; the mechanism is as follows: apoptosis of drug-resistant tumour cells is induced through up regulation of expression of proapoprotic protein is up-regulated and down regulation of expression of inhibitor of apoptosis protein; tumour drug-resistant protein P-gp expression is inhibited by increasing active oxygen expression level of drug-resistant tumour cells, so that tumour multi-drug drug-resistant cells are overcome, and therefore, the salvianolic acid A disclosed by the invention has the application prospect for preparing the medicine for overcoming tumour multi-drug resistance.

Description

technical field [0001] The invention relates to the application of salvianolic acid A in antitumor, in particular to the application of salvianolic acid A in the preparation of drugs for treating multidrug resistance of tumors. Background technique [0002] The Ministry of Health of the People's Republic of China conducted the third national retrospective sampling survey on causes of death, showing that malignant tumors are the second leading cause of death among Chinese residents. The occurrence of tumor is a complicated and long-term process, which is affected by many factors inside and outside the body. Cells form malignant cells through processes such as proliferation, differentiation, dysplasia, transformation, and anamorphosis. In the chemotherapy of various tumors, tumor multidrug resistance is an important factor affecting the effect of chemotherapy, and can lead to recurrence and metastasis, which has become the biggest obstacle to tumor treatment and a major reason...

Claims

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Application Information

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IPC IPC(8): A61K31/216A61P35/00
Inventor 王昕王春艳卢光明孟庆欣朱飞鹏张龙江张志强牛红梅黄嘉逸奚海燕盛会雪
Owner NANJING GENERAL HOSPITAL NANJING MILLITARY COMMAND P L A
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