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Fibroin-based biological wound dressing and preparation method thereof

A technology of wound dressing and silk fibroin, which is applied in bandages, absorbent pads, medical science, etc., can solve the problems of no compound preparation of high-mechanical performance biological dressings, and achieve good safety performance and biocompatibility, uniform length, and safety Good results

Active Publication Date: 2014-04-09
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is currently no method for combining chitosan and silk fibroin microfibers to prepare bio-dressings with high mechanical properties

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The preparation method of the biological wound dressing in this embodiment includes the following steps in sequence.

[0023] (1) Weigh 10g of shredded cocoon layer in 0.5% Na 2 CO 3 Degumming in boiling aqueous solution twice, each time for 30 minutes, to obtain silk fibroin fibers, rinsed with deionized water and dried in an oven.

[0024] (2) Degrading the silk fiber obtained in step (1) with a 0.1-4 mol / L NaOH solution for 3-24 hours to obtain a silk suspension. In the present invention, the silk fibroin fiber in step (1) can be degraded in a certain concentration of NaOH solution for a certain period of time to obtain silk fibroin microfibers: the concentration of NaOH used is 0.1-4mol / L, preferably 0.5-2mol / L, the most The best is 1mol / L; the bath ratio used is 1:(50~200) (g / ml), preferably 1:(75~150), and the best is 1:100; the time used is 3~24h, preferably 6~ 18h, the best is 12h.

[0025] (3) Wash the silk fibroin suspension obtained in step (2) several ti...

Embodiment 2

[0030] The preparation method of the biological wound dressing in this embodiment includes the following steps in sequence.

[0031] (1) Weigh 10g of shredded cocoon layer in 0.5% Na 2 CO 3 Degumming in boiling aqueous solution twice, each time for 30 minutes, to obtain silk fibroin fibers, rinsed with deionized water and dried in an oven.

[0032] (2) Degrade the silk fiber obtained in step (1) with 0.1 mol / L NaOH solution for 24 hours to obtain a silk suspension, the bath ratio used is 1:50 (g / ml).

[0033] (3) The silk fibroin suspension obtained in step (2) was washed once with deionized water, the supernatant was discarded, dried in an oven and then ground to obtain silk fibroin microfibers.

[0034] (4) Dissolve 0.4g of chitosan in 20ml of 0.3mol / L acetic acid solution, then add the silk fibroin microfibers obtained in step (3) into the chitosan solution, and mix well. Wherein the mass of silk fibroin accounts for 10% of the mass of chitosan.

[0035] (5) Spread the ...

Embodiment 3

[0038] The preparation method of the biological wound dressing in this embodiment includes the following steps in sequence.

[0039] (1) Weigh 10g of shredded cocoon layer in 0.5% Na 2 CO 3 Degumming in boiling aqueous solution twice, each time for 30 minutes, to obtain silk fibroin fibers, rinsed with deionized water and dried in an oven.

[0040] (2) Degrade the silk fiber obtained in step (1) with 1mol / L NaOH solution for 24 hours to obtain a silk suspension, the bath ratio used is 1:100 (g / ml).

[0041] (3) The silk fibroin suspension obtained in step (2) was washed 4 times with deionized water, the supernatant was discarded, dried in an oven and then ground to obtain silk fibroin microfibers.

[0042] (4) Dissolve 0.4g of chitosan in 20ml of 0.3mol / L acetic acid solution, then add the silk fibroin microfibers obtained in step (3) into the chitosan solution, and mix well. Wherein the mass of silk fibroin microfiber accounts for 50% of the mass of chitosan.

[0043] (5)...

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Abstract

The invention relates to a fibroin-based biological wound dressing and a preparation method thereof. The preparation method comprises the steps of degumming a cocoon shell in a Na2CO2 boiling water solution with a weight percent of 0.5 percent twice with 30min each time, drying the obtained fibroin fibers by using a drying oven after being washed with deionized water, and dissolving the obtained fibroin fibers by using a 0.1-4mol / L NaOH solution for 3-24h to obtain a fibroin suspending liquid, wherein the used bath ratio is 1:(50-200)(g / ml). The fibroin microfibers are obtained by using an alkaline hydrolysis method, and are uniform in length. In a case that any toxic chemical crosslinking agent is not added, the biological wound dressing is prepared; the preparation method is simple in process and good in safety. The biological wound dressing prepared by adopting the preparation method has the characteristic of high mechanical property. The invention provides a method for preparing the biological wound dressing with high mechanical property. The biological wound dressing has good biocompatibility and is capable of effectively recovering a wound.

Description

technical field [0001] The invention belongs to a processing technology of a biological wound dressing, in particular to a preparation method of a dressing with high mechanical properties. Background technique [0002] Severe skin wounds caused by burns or other causes often bring great pain to patients, and treating wounds with wound dressings is an important step in early treatment. An ideal wound dressing can effectively isolate bacteria and dust, and requires the dressing to have certain moisture retention and good biocompatibility ([J] Acta Biomaterials, 2012, 8(8): 3049-3060). Chitosan is a linear polysaccharide linked by β-(1,4) glycosidic bonds, which has good biocompatibility, biodegradability, and non-toxicity ([J] Advanced Drug Delivery Reviews, 2010, 62 (1 ):3-11). Various forms of chitosan wound dressings have been widely developed, such as non-woven fabrics, gels, films and scaffolds ([J] Biotechnology Advances, 2008, 26(1):1-21). However, the disadvantages ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/32A61L15/28
Inventor 朱良均施李杨许宗溥杨明英张海萍
Owner ZHEJIANG UNIV
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