High-sensitivity TMB (tetramethylbenzidine) color developing liquid and preparation method thereof

A high-sensitivity, color-developing liquid technology, applied in the field of high-sensitivity TMB color-developing solution and its preparation, can solve the problems of low sensitivity, inconvenience for patients, and difficulty in accurately detecting patients infected with HCV, etc.

Active Publication Date: 2014-04-09
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

By measuring the absorbance, the content of the detected substance can be determined qualitatively or quantitatively. Most of the chromogenic solutions on the market that use TMB as the chromogenic

Method used

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  • High-sensitivity TMB (tetramethylbenzidine) color developing liquid and preparation method thereof
  • High-sensitivity TMB (tetramethylbenzidine) color developing liquid and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0027] A kind of highly sensitive TMB chromogenic solution of the present invention, the concentration of its each component is:

[0028] Chromogenic solution A:

[0029] Tris (Tris) 12.11g / L

[0030] Potassium ferrocyanide 1g / L

[0031] Sodium pyrophosphate 5g / L

[0032] Hydrogen peroxide 1mL / L

[0033] Chromogenic solution B:

[0034] NaH 2 PO 4 2H 2 O 2.96g / L

[0035] Na 2 HPO 4 12H 2 O 29g / L

[0036] TMB 5g / L

[0037] Dimethyl sulfoxide 10mL / L

[0038] Disodium EDTA 1 g / L

[0039] Lauryl dimethyl betaine 0.1g / L

[0040] The preparation method of a high-sensitivity TMB chromogenic solution described in this example is as follows: take 12.11g of Tris (Tris), 1g of potassium ferrocyanide and 5g of sodium pyrophosphate in solution A, and place them in the Dissolve in 1L of distilled water with slow stirring, then add 1mL of hydrogen peroxide to the mixture, stir for 30 minutes, wait until it is completely stirred evenly, and store in an environment of 2-8°C. T...

Embodiment 3

[0047] A kind of highly sensitive TMB chromogenic solution of the present invention, the molar concentration of its each component is:

[0048] Chromogenic solution A:

[0049]Tris (Tris) 12.11g / L

[0050] Potassium ferrocyanide 1g / L

[0051] Sodium pyrophosphate 5g / L

[0052] Hydrogen peroxide 1mL / L

[0053] Chromogenic solution B:

[0054] NaH 2 PO 4 ·H 2 O 2.96g

[0055] Na 2 HPO 4 12H 2 O 29g

[0056] TMB 5g / L

[0057] Disodium EDTA 2 g / L

[0058] Dimethyl sulfoxide 10mL / L

[0059] Lauryl dimethyl betaine 1g / L

[0060] The preparation method of a high-sensitivity TMB chromogenic solution described in this example is as follows: take 12.11g of Tris (Tris), 1g of potassium ferrocyanide and 5g of sodium pyrophosphate in solution A, and place them in the Dissolve in 1L of distilled water with slow stirring, then add 1mL of hydrogen peroxide to the mixture, stir for 30 minutes, and store it in an environment of 2-8°C after it is completely stirred and evenly pla...

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PUM

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Abstract

The invention provides high-sensitivity TMB (tetramethylbenzidine) color developing liquid and a preparation method thereof, aiming at the defects that color developing liquid adopting TMB (tetramethylbenzidine) as a color developing substrate in the market has the weakness that the sensitivity is low. The high-sensitivity TMB color developing liquid is characterized in that the concentration of each component is as follows: in color developing liquid A, the concentration of Tris is 12.11g/L, the concentration of potassium ferrocyanide is 1g/L, the concentration of sodium pyrophosphate is 5g/L, and the concentration of hydrogen peroxide is 1ml/L; in color developing liquid B, the concentration of NaH2PO4.2H2O is 2.96g/L, the concentration of Na2HPO4.12H2O is 29g/L, the concentration of TMB is 5g/L, the concentration of disodium edetate dihydrate is 1g/L to 2g/L, the concentration of dimethyl sulfoxide is 10mL/L, and the concentration of dodecyl dimethyl betaine is 0.1g/L to 1g/L. The high-sensitivity TMB color developing liquid has the beneficial effects that the sensitivity of detecting hepatitis c through euzymelinked immunosorbent assay is greatly improved, and the practicability is very important.

Description

technical field [0001] The invention relates to clinical in vitro detection technology, in particular to a high-sensitivity TMB chromogenic solution and a preparation method thereof. Background technique [0002] The main detection methods of HCV antigen are fluorescence immunoassay, enzyme-linked immunoassay and radioimmunoassay. Among them, enzyme-linked immunosorbent assay has the advantages of strong specificity, simple operation, and low cost, and has been well received by people. However, after ordinary HCV infection, there is generally a long window period until the antibody turns positive, an average of 70 days, and some patients have a window period. It can be extended to 6-9 months or longer, and about 1% to 3% of patients in the window period can continue to be negative for HCV. The detection method can take advantage of the early replication of the patient's genome and the appearance of viremia a few days after infection, which is helpful for the early detection...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N33/576
Inventor 谭柏清罗维晓甘宜梧
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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