High-sensitivity TMB (tetramethylbenzidine) color developing liquid and preparation method thereof

Abstract

The invention provides high-sensitivity TMB (tetramethylbenzidine) color developing liquid and a preparation method thereof, aiming at the defects that color developing liquid adopting TMB (tetramethylbenzidine) as a color developing substrate in the market has the weakness that the sensitivity is low. The high-sensitivity TMB color developing liquid is characterized in that the concentration of each component is as follows: in color developing liquid A, the concentration of Tris is 12.11g / L, the concentration of potassium ferrocyanide is 1g / L, the concentration of sodium pyrophosphate is 5g / L, and the concentration of hydrogen peroxide is 1ml / L; in color developing liquid B, the concentration of NaH2PO4.2H2O is 2.96g / L, the concentration of Na2HPO4.12H2O is 29g / L, the concentration of TMB is 5g / L, the concentration of disodium edetate dihydrate is 1g / L to 2g / L, the concentration of dimethyl sulfoxide is 10mL / L, and the concentration of dodecyl dimethyl betaine is 0.1g / L to 1g / L. The high-sensitivity TMB color developing liquid has the beneficial effects that the sensitivity of detecting hepatitis c through euzymelinked immunosorbent assay is greatly improved, and the practicability is very important.

Description

technical field [0001] The invention relates to clinical in vitro detection technology, in particular to a high-sensitivity TMB chromogenic solution and a preparation method thereof. Background technique [0002] The main detection methods of HCV antigen are fluorescence immunoassay, enzyme-linked immunoassay and radioimmunoassay. Among them, enzyme-linked immunosorbent assay has the advantages of strong specificity, simple operation, and low cost, and has been well received by people. However, after ordinary HCV infection, there is generally a long window period until the antibody turns positive, an average of 70 days, and some patients have a window period. It can be extended to 6-9 months or longer, and about 1% to 3% of patients in the window period can continue to be negative for HCV. The detection method can take advantage of the early replication of the patient's genome and the appearance of viremia a few days after infection, which is helpful for the early detection...

AI Technical Summary

Problems solved by technology

By measuring the absorbance, the content of the detected substance can be determined qualitatively or quantitatively. Most of the chromogenic solutions on the market that use TMB as the chromogenic substrate show the disadvantage of low sensitivity, and it is difficult to accurately detect the presence of Patients infected with HCV during the window period bring great inconvenience to patients

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