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Populus tremula grey speck disease germ spore production culture medium as well as preparation method and application method thereof

A technology of poplar gray spot and culture medium, applied in the direction of using spores, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problem that the fungal culture medium cannot meet the needs of the growth and development of poplar gray spot bacteria Spores and other problems, to achieve the effect of stable material performance, simple process and easy realization

Inactive Publication Date: 2014-04-16
NORTHEAST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problem that the existing fungal culture medium cannot well satisfy the growth and development of gray spot fungus to produce spores, and provides a spore-producing medium for gray spot fungus and its preparation method and use method

Method used

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  • Populus tremula grey speck disease germ spore production culture medium as well as preparation method and application method thereof
  • Populus tremula grey speck disease germ spore production culture medium as well as preparation method and application method thereof
  • Populus tremula grey speck disease germ spore production culture medium as well as preparation method and application method thereof

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specific Embodiment approach 1

[0016] Specific embodiment one: in the present embodiment, the sporulation culture medium of poplar gray spot fungus, every 1L of poplar gray spot fungus sporulation culture medium is made of potato 200.0g~202.0g, peptone 0.4g~0.6g, potassium dihydrogen phosphate 2.9g-3.1g, magnesium sulfate 1.4g-1.6g, agar 16g-20g and the rest distilled water.

specific Embodiment approach 2

[0017] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that the pH of the spore-forming medium for P. cinerea is 6.5-7.5. Others are the same as the first embodiment.

specific Embodiment approach 3

[0018] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is that every 1L of poplar gray spot fungus sporulation culture medium is made of potato 201g, peptone 0.5g, dipotassium hydrogen phosphate 3g, magnesium sulfate 1.53 g, 20g of agar and the remainder of distilled water. Others are the same as those in Embodiment 1 or 2.

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Abstract

The invention discloses a populus tremula grey speck disease germ spore production culture medium as well as a preparation method and an application method thereof, relating to a plant pathogen fungus spore production culture medium as well as an preparation method and an application method thereof, and aiming at solving the problem that an existing fungus culture medium cannot well meet the requirement for the spore production through the growth and development of populus tremula grey speck disease germs. The spore production culture medium is prepared from potato, peptone, monopotassium phosphate, magnesium sulfate, agar and the balance of distilled water. The preparation method of the spore production culture medium comprises the following steps of peeling potatoes, boiling the peeled potatoes for 20 minutes to 30 minutes, filtering to obtain the filter liquid, dissolving the peptone, the monopotassium phosphate, the magnesium sulfate and the agar into the filter liquid, and fixing the volume to 1L by utilizing the distilled water so as to complete the preparation of the populus tremula grey speck disease germ spore production culture medium. The culture conditions of the culture medium are as follows: the temperature is 19 to 30 DEG C, and the culture period is 7 to 30 days. The populus tremula grey speck disease germ spore production culture medium as well as the preparation method and the application method disclosed by the invention are applied to the field of microorganism culture.

Description

technical field [0001] The present invention relates to a plant pathogenic fungus sporulation medium and its preparation method and use method, mainly related to the sporulation medium of poplar gray spot fungus (Sporocadus populinus Orsenigo, Rodondi&B.Sutton=Coryneum populinum Bres.) and its preparation method and how to use it. Background technique [0002] Poplar (Populus spp.) has the characteristics of fast growth, early maturity, strong adaptability, easy renewal, good material and wide application, so it is planted in large areas in the north. Poplar is the main species of timber forest, shelter forest and urban greening in northern my country. [0003] Poplar gray spot (S.populinus) is a great threat to poplar seedling cultivation and afforestation. It is the most common and serious disease in the seedling stage of poplar. It mainly occurs on the leaves, tips and stems of annual seedlings and saplings. The causative bacterium of poplar gray spot disease (S.populin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N3/00C12R1/645
Inventor 马玲刘雪英刘雪峰沙依拉·帕那西晁开瑞
Owner NORTHEAST FORESTRY UNIVERSITY
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