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Molecular marker for detecting specific mutation of rice aroma control gene and application of molecular marker

A molecular marker and a specific technology, applied in the field of molecular genetics, can solve the problems of electrophoresis detection technology, such as many steps in gel production, cumbersome printing and dyeing process, unfavorable wide application, etc., to achieve effective and rapid detection, shortened detection time, and obvious effect.

Inactive Publication Date: 2014-04-16
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The second exon deletion was discovered by Chen et al. in 2008. Another allele of Badh2, that is, there is a 7-base deletion in the second exon. Through gene function complementation, it was verified that the deletion caused the aroma of rice.
However, the electrophoretic detection technology has defects such as many gel preparation steps, long electrophoresis time, cumbersome printing and dyeing process, etc., which is not conducive to the wide application of these molecular markers in marker-assisted selection fragrance breeding.

Method used

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  • Molecular marker for detecting specific mutation of rice aroma control gene and application of molecular marker
  • Molecular marker for detecting specific mutation of rice aroma control gene and application of molecular marker
  • Molecular marker for detecting specific mutation of rice aroma control gene and application of molecular marker

Examples

Experimental program
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Effect test

Embodiment 1

[0037] 1.1 Extract DNA from rice leaves to be tested

[0038] Select the rice to be tested: 1-fragrant rice: Taihu fragrant japonica; 2-fragrant rice: Zaoxiang 09-2; 3-fragrant rice: Zixiangnuo 861; 4-non-fragrant rice: Xiushui 123; 5, 6-two Heterozygous plants (i.e. hybrid plants of aromatic rice and non-aromatic rice)

[0039] Among them, the heterozygous plants, that is, the heterozygous plants of fragrant rice and non-fragrant rice, are the hybrid offspring of Xiushui 123 and Taihu Xiangjing, and the hybrid offspring of Zixiangnuo 861 and Xiushui 123, respectively.

[0040] Take rice leaves 2-3cm into 2.0ml centrifuge tubes, put them into liquid nitrogen, and grind them; add 700ml of CTAB extract, put them in a water bath at 65°C for 30 minutes, and shake them repeatedly during this period; add 700ml of chloroform, and mix them upside down Uniformly separate into three layers; centrifuge at 12000rpm / min for 15min; draw 600ml of supernatant into a 1.5ml centrifuge tube, ad...

Embodiment 2

[0056] Select rice varieties to be tested: Guangling Xiangnuo, Guangling Xiangjing, Xiangjing 103, Xiangxuenuo, Shenxiangjing 4, Zixiangnuo 863, Yuexiangzhan, Nongxiang 46 and other varieties;

[0057] All the other detection methods are the same as in Example 1, through electrophoresis on 2% agarose gel for 25-35min, 220V, detection, both 591bp and 169bp two characteristic bands appear simultaneously, that is, the rice to be tested can be provided by the present invention Molecular marker detection, identified as the fragrant rice variety with the specific mutation.

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Abstract

The invention discloses a molecular marker for detecting the specific mutation of a rice aroma control gene. The molecular marker can detect whether the mutation occurs in rice plants and is applicable to the breeding of rice varieties and the screening of aroma rice resources.

Description

technical field [0001] The invention belongs to the field of molecular genetics, and specifically relates to a molecular marker for detecting a specific mutation of a rice aroma control gene and its application. Background technique [0002] Aroma is an important food quality trait of rice, and rice aroma has long been regarded as an important indicator of rice quality in the world. Basmati rice is loved by consumers for its delicate taste and rich nutritional value, and has good economic benefits and market prospects. Therefore, it is a good breeding direction for breeders to select and breed new varieties of fragrant rice. [0003] So far, genetic research on rice aroma has made great progress. From genetic analysis and positioning to gene cloning, to the application of molecular marker-assisted selection technology, it provides great convenience for the breeding of new varieties of aromatic rice. [0004] Aroma traits in rice are controlled by recessive genes in the nu...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 吴书俊闫影曹黎明赵志鹏万常照
Owner SHANGHAI ACAD OF AGRI SCI