Test paper and method for detecting florfenicol and thiamphenicol

A technology of thiamphenicol and test paper, which is applied in the field of test paper for testing florfenicol and thiamphenicol, can solve the problems that are not suitable for low-cost testing needs of enterprises, high requirements for testing personnel, and complicated testing equipment. Achieve the effects of short detection time, wide application range and high sensitivity

Active Publication Date: 2014-04-16
BEIJING KWINBON BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The physical and chemical detection methods include gas chromatography, gas chromatography-mass spectrometry, etc. The physical and chemical detection methods have high accuracy, but they have the disadvantages of high detection cost, complicated detection equipment, high requirements for detection personnel, and long detection time; currently commonly used Immunological analysis methods, mainly enzyme-linked immunoassays, require high testing costs and are not suitable for low-cost testing needs of enterprises.

Method used

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  • Test paper and method for detecting florfenicol and thiamphenicol
  • Test paper and method for detecting florfenicol and thiamphenicol
  • Test paper and method for detecting florfenicol and thiamphenicol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1, detect the composition of the test paper of florfenicol and thiamphenicol

[0040] 1. Microporous Reagents

[0041] The micropore reagent has a micropore plug;

[0042] The thiamphenicol drug monoclonal antibody-colloidal gold marker is lyophilized on the micropore reagent, and the coating amount is 0.15-0.25 μg / ml.

[0043] Two, test strips ( figure 1 ):

[0044] The test strip is composed of a bottom plate, a sample absorption pad, a reaction film, a water absorption pad, and a protective film;

[0045] The sample absorbent pad 1, reaction film 2, water absorbent pad 3 and protective film 7 are pasted on the bottom plate 6 in sequence, the end of the sample absorbent pad is connected to the reaction film, and the end of the reaction film is connected to the water absorbent pad. The beginning of the absorbent pad is aligned with the beginning of the bottom plate, and the end of the absorbent pad is aligned with the end of the bottom plate;

[0046] ...

Embodiment 2

[0050] The preparation method of test paper described in embodiment 2, embodiment 1

[0051] 3. Preparation of test paper

[0052] The preparation method of this test paper mainly comprises the steps:

[0053] 1) Preparation of freeze-dried microporous reagents with thiamphenicol drug monoclonal antibody-colloidal gold markers;

[0054] 2) Prepare a reaction membrane with a detection area coated with thiamphenicol hapten-carrier protein conjugate and a quality control area coated with goat anti-mouse anti-antibody;

[0055] 3) Assemble the reaction film prepared in 2) with the sample absorbent pad, water absorbent pad, protective film, and bottom plate into a test strip;

[0056] 4) Assemble the freeze-dried microwell reagents and test strips prepared in 1) and 3) with thiamphenicol drug monoclonal antibody-colloidal gold markers.

[0057] The following step-by-step detailed description:

[0058] (1) Preparation of each component

[0059] 1. Synthesis and identification o...

Embodiment 3

[0106] Embodiment 3, the application of test paper

[0107] One, detect florfenicol and thiamphenicol in milk with test paper described in embodiment 1

[0108] The test paper of the invention can detect milk samples. The general detection method is as follows:

[0109] 1. Detection method

[0110] Add the sample solution to be detected dropwise into the micropore reagent, mix well, insert the test strip with the end of the test paper marked with the MAX line facing down into the micropore reagent, and watch the result within 5 minutes.

[0111] 2. Judgment of test results

[0112] When the concentration of florfenicol and thiamphenicol in the sample is higher than or equal to the minimum detection limit of the test paper, the colloidal gold antibody will bind to florfenicol or thiamphenicol, so that it will not interact with formazan in the detection area due to competition. Combination of sulfomycin hapten conjugate without red band is positive. Due to the lack of antib...

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Abstract

The invention discloses test paper and a method for detecting florfenicol and thiamphenicol. The test paper comprises a micropore reagent and a test strip, the micropore reagent comprises freeze-dried colloidal-gold-labelled thiamphenicol medicament specific antibody, wherein the thiamphenicol medicament specific antibody is thiamphenicol medicament monoclonal antibody or thiamphenicol medicament polyclonal antibody; the test strip is formed by successively connecting a sample absorption pad, a reaction membrane, a water absorption pad, a protective membrane and a base plate, the reaction membrane comprises a detection zone and a quality control zone, the detection zone is coated with thiamphenicol hapten-carrier protein conjugate, and the quality control zone is coated with antiantibody. The method for detecting florfenicol and thiamphenicol by using the test paper provided by the invention is simple, rapid, visualized, accurate, wide in application scope, low in cost and easy to popularize and use.

Description

technical field [0001] The invention relates to a test paper and a method for detecting florfenicol and thiamphenicol. Background technique [0002] Florfenicol, thiamphenicol and chloramphenicol are all chloramphenicol drugs, and thiamphenicol is a derivative of florfenicol, and their antibacterial effects are similar to chloramphenicol. Due to the serious side effects of chloramphenicol, the use of chloramphenicol has been banned or strictly restricted in the world. Both florfenicol and thiamphenicol have high antibacterial activity, and they are currently replacing chloramphenicol. They are mainly used to treat bacterial diseases in fish, pigs, cattle and poultry, and have been used on a large scale in the breeding industry. However, the extensive application of florfenicol and thiamphenicol in livestock and poultry production also leads to their residues in livestock and poultry products, endangering public health. The EU (EEC) Directive 96 / 23 stipulates that the MRL v...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/558
CPCG01N33/531G01N33/558G01N33/577
Inventor 何方洋罗晓琴吴鹏冯静蒲小容田甜陈炜玲
Owner BEIJING KWINBON BIOTECH
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