Compound microorganism agent, application of compound microorganism agent in degradation of humic acid and application method of compound microorganism agent

A technology of compound microbial inoculants and humic acid, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effects of no by-products, simple and easy process flow, and expanded application scope.

Inactive Publication Date: 2014-04-23
AGRI BIOTECH RES CENT OF SHANXI PROVINCE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no effective way to degrade humic acid

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Eight strains of Bacillus subtilis, Bacillus licheniformis, Bacillus pumilus, Saccharomyces cerevisiae, Lactobacillus plantarum, Lactobacillus acidophilus, Streptococcus thermophilus and Acetobacter were inoculated and cultured in PDA medium respectively, and the culture temperature was controlled at 27- 35°C, culture time 24-36 hours, pH value is natural; among them, aerobic bacteria are cultured in shake flasks, and anaerobic bacteria are cultured statically; the number of viable bacteria in each medium is detected, and the number of bacteria is greater than 10 9 cfu / mL yeast greater than 10 7 cfu / mL stop culture;

[0028] According to the weight ratio of the activated strains, Bacillus subtilis: Bacillus licheniformis: Bacillus pumilus: Saccharomyces cerevisiae: Lactobacillus plantarum: Lactobacillus acidophilus: Streptococcus thermophilus: Acetobacter = 2:2:2:1: Mix 1:1:1:1 to get a complex microbial agent.

Embodiment 2

[0030] Eight strains of Bacillus subtilis, Bacillus licheniformis, Bacillus pumilus, Saccharomyces cerevisiae, Lactobacillus plantarum, Lactobacillus acidophilus, Streptococcus thermophilus and Acetobacter were inoculated and cultured in PDA medium respectively, and the culture temperature was controlled at 27- 35°C, culture time 24-36 hours, pH value is natural; among them, aerobic bacteria are cultured in shake flasks, and anaerobic bacteria are cultured statically; the number of viable bacteria in each medium is detected, and the number of bacteria is greater than 10 9 cfu / mL yeast greater than 10 7 cfu / mL stop culture;

[0031] According to the weight ratio of the activated strains, Bacillus subtilis: Bacillus licheniformis: Bacillus pumilus: Saccharomyces cerevisiae: Lactobacillus plantarum: Lactobacillus acidophilus: Streptococcus thermophilus: Acetobacter = 1.8: 1.8: 1.8: 0.5: 1.2: 1.2: 1.2: 0.6 mixed to obtain a composite microbial agent.

Embodiment 3

[0033] Eight strains of Bacillus subtilis, Bacillus licheniformis, Bacillus pumilus, Saccharomyces cerevisiae, Lactobacillus plantarum, Lactobacillus acidophilus, Streptococcus thermophilus and Acetobacter were inoculated and cultured in PDA medium, and the culture temperature was controlled at 27- 35°C, culture time 24-36 hours, pH value is natural; among them, aerobic bacteria are cultured in shake flasks, and anaerobic bacteria are cultured statically; the number of viable bacteria in each medium is detected, and the number of bacteria is greater than 10 9cfu / mL yeast greater than 10 7 cfu / mL stop culture;

[0034] According to the weight ratio of the activated strains, Bacillus subtilis: Bacillus licheniformis: Bacillus pumilus: Saccharomyces cerevisiae: Lactobacillus plantarum: Lactobacillus acidophilus: Streptococcus thermophilus: Acetobacter = 1.4: 1.4: 1.4: 0.8: 0.7:0.7:0.7:0.3 mixed to obtain a composite microbial agent.

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Abstract

The invention relates to a compound microorganism agent and application thereof, in particular relates to a compound microorganism agent, application of the compound microorganism agent in degradation of humic acid and an application method of the compound microorganism agent, aims at solving the problems of difficult degradation and low degradation rate of humic acid, provides the compound microorganism agent and application of the agent in the degradation of humic acid and provides a method for using the compound microorganism agent to degrade humic acid into micromolecules. The compound microorganism agent comprises bacillus, saccharomyces cerevisiae, lactobacillus and bacillus aceticus, wherein the bacillus comprises bacillus subtilis, bacillus licheniformis and bacillus pumilus; the lactobacillus comprises lactobacillus plantarum, lactobacillus acidophilus and streptococcus thermophilus. Due to the adoption of the agent, macromolecules of humic acid can be degraded into micromolecules.

Description

technical field [0001] The invention relates to a composite microbial bacterial agent and its application, in particular to a composite microbial bacterial agent, an application of the microbial bacterial agent to degrade humic acid, and a method for using the bacterial agent to degrade humic acid. Background technique [0002] Weathered coal, lignite, and peat contain a large amount of humic acid (black humic acid, brown humic acid, and fulvic acid). The conventional method is to extract with lye and precipitate with sulfuric acid, but the large molecules of black humic acid and brown humic acid are insoluble. In water, the utilization efficiency is low, and fulvic acid is easy to be utilized, but the content is limited. The contents and proportions of the three kinds of humic acids vary greatly in weathered coal and lignite in different regions, so the application is limited. Humic acid is a complex organic matter formed by long-term physical, chemical, and biological act...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/18C12P39/00C12R1/865C12R1/125C12R1/10C12R1/07C12R1/25C12R1/23C12R1/46
Inventor 梁宏张纹歌黄静赵佳陈哲张萌张瑜
Owner AGRI BIOTECH RES CENT OF SHANXI PROVINCE
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