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Mycobacterium drug sensitive detection kit and testing method thereof

A detection kit and mycobacteria detection technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of inability to guide clinical medication in a timely and effective manner, limit popularization and application, and delay the patient's condition, and achieve the goal of testing Wide range of drugs, convenient operation, and the effect of reducing the generation of drug-resistant strains

Inactive Publication Date: 2014-04-23
ZHUHAI ENCODE MEDICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Failure to guide clinical medication in a timely and effective manner, delaying the patient's condition
The World Health Organization strongly advocates the use of liquid culture medium for drug susceptibility testing, but the current clinically available liquid-based drug susceptibility testing reagents require expensive equipment and high testing costs, which limits their popularization and application.
At the same time, there are a large amount of literature on the liquid-based detection method of mycobacterial trace drug susceptibility, but there is no commercial commercial box available for clinical use in China at present.

Method used

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  • Mycobacterium drug sensitive detection kit and testing method thereof
  • Mycobacterium drug sensitive detection kit and testing method thereof
  • Mycobacterium drug sensitive detection kit and testing method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0023] In this embodiment, the present invention includes the following parts:

[0024] 1. The mycobacterial susceptibility medium is filled into 10ml / cartridges in brown glass screw-top bottles, 20 bottles / box in total.

[0025] 2. The Mycobacterium susceptibility test plate is a 26-well microplate, with a total of 20 strips, each of which is sealed by a sterile three-side-sealed aluminum foil bag, and one sterile suction nozzle is sealed at the same time for the convenience of customers. Schematic diagram of a 26-well microplate figure 2 shown.

[0026] 3. The sterile diluent is 3ml / cartridge of potassium salt and sodium salt sterile solution, filled with anti-theft drop bottles.

[0027] 4. The bacteria inhibitors are freeze-dried preparations of polymyxin B, amphotericin B, nalidixic acid, azlocillin and trimethoprim, and polymyxin B, amphotericin B, nalidixic acid The mixed drug solution of , azlocillin and trimethoprim is prepared by vacuum freeze-drying process.

...

Embodiment 2

[0035] In this embodiment, the present invention includes the following parts:

[0036] 1. The mycobacterial susceptibility medium is filled into 20ml / cartridges in brown glass screw-top bottles, 10 bottles / box in total.

[0037] 2. The mycobacteria susceptibility test plate is a 96-well microplate, with a total of 10 strips. Each strip is sealed by a sterile three-side-sealed aluminum foil bag, and one sterile suction nozzle is sealed at the same time for the convenience of customers.

[0038] 96-well microplate drug concentration distribution table

[0039]

[0040] 3. The sterile diluent is 3ml / cartridge of potassium salt and sodium salt sterile solution.

[0041] 4. The bacteria inhibitors are freeze-dried preparations of polymyxin B, amphotericin B, nalidixic acid, azlocillin and trimethoprim.

[0042] 5. Use the mycobacterium drug susceptibility detection kit to conduct the drug susceptibility test of mycobacteria:

[0043] (1) Take out the freeze-dried bacteria in...

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Abstract

The invention discloses a mycobacterium drug sensitive detection kit which is safe and effective, convenient to operate, short in testing time and wide in testing drug range and a testing method thereof. The mycobacterium drug sensitive detection kit provided by the invention comprises a kit body (1), wherein the kit body (1) internally comprises a plurality of drug sensitive culture mediums (2), a drug sensitive testing plate (3), a sterile suction nozzle (4), a sterile diluent (5), an infectious microbe inhibitor (6), a specification and a report label, the drug sensitive culture medium (2) comprises a basal culture medium, a growth promoter and a bacteriostatic agent, and a negative control hole (31), a positive control hole (32) and a plurality of drug containing holes (33) are formed in the drug sensitive testing plate (3), wherein the drug containing holes (33) are arranged into a matrix and are coated with different antituberculosis drugs. The mycobacterium drug sensitive detection kit can be applied to the field of mycobacterium tuberculosis detection.

Description

technical field [0001] The invention relates to an in vitro diagnostic reagent, in particular to a mycobacterium drug susceptibility detection kit and a testing method thereof. Background technique [0002] Tuberculosis is the main killer of infectious diseases in the world today, and has posed a serious challenge to international public health. According to the report of the World Health Organization, there are currently 2 billion people infected with tuberculosis in the world, and about 3 million people die of tuberculosis every year. Tuberculosis, which kills more adults each year than AIDS, malaria and tropical diseases combined. The emergence of drug-resistant tuberculosis, especially multidrug-resistant (MDR) and severely multidrug-resistant (XDR) tuberculosis, is one of the main reasons for the resurgence of tuberculosis. MDR tuberculosis is resistant to isoniazid and rifampicin, the two most important anti-tuberculosis drugs at present, and XDR tuberculosis is resi...

Claims

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Application Information

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IPC IPC(8): C12Q1/18
Inventor 孙宜峰王珍曾冰冰
Owner ZHUHAI ENCODE MEDICAL ENG
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