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A method for detecting enrofloxacin metabolites in sea cucumbers

A technology for metabolites and sea cucumbers, which is applied in measurement devices, material analysis by electromagnetic means, instruments, etc., can solve the problems of fast analysis speed, high price and high maintenance cost, and achieve the effect of high sensitivity

Active Publication Date: 2016-08-10
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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Problems solved by technology

Time-of-flight mass spectrometry belongs to high-resolution mass spectrometry, which can help the identification of unknown compounds and the distinction of m / z approximate ions. The analysis speed is fast, but it is expensive, more precise, and has high maintenance costs.

Method used

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  • A method for detecting enrofloxacin metabolites in sea cucumbers
  • A method for detecting enrofloxacin metabolites in sea cucumbers
  • A method for detecting enrofloxacin metabolites in sea cucumbers

Examples

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Embodiment

[0071] Example Determination of metabolites of enrofloxacin in the process of nitrogen deethylation metabolism in sea cucumbers

[0072] This example is a method for analyzing enrofloxacin metabolites in sea cucumbers by using high performance liquid chromatography-tandem mass spectrometry-ion trap mass spectrometry (LC-MS / MS-Qtrap) technology. Sea cucumber samples were dehydrated with anhydrous sodium sulfate, and enrofloxacin and its metabolites in the samples were extracted with acidified acetonitrile. / MS-Qtrap) method to analyze the enrofloxacin metabolites and their structures in sea cucumbers.

[0073] Enrofloxacin has a variety of metabolic pathways in the body and is converted into a variety of metabolites. Under the action of various enzymes in the body, N-oxidation reactions, N-dealkylation reactions, and N-acetylation reactions can occur And chemical reactions such as the degradation of piperazine ring. Studies have shown that the metabolites of enrofloxacin in d...

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Abstract

A method for detecting enrofloxacin metabolites in sea cucumbers belongs to the technical field of aquatic product detection, and includes sample extraction, purification, drawing of standard curves, instrument conditions used in experiments, and structural analysis of metabolites. The present invention applies liquid chromatography-tandem mass spectrometry-ion trap technology, adopts pMRM method to accurately measure predicted metabolites, and can detect low-concentration metabolites; In this way, both the MRM chromatogram and the MS2 mass spectrum of the corresponding metabolites can be obtained. The invention can detect 10 kinds of metabolites including ciprofloxacin, and the mass spectrum response intensity of two newly discovered metabolites is far greater than that of ciprofloxacin. The invention fills up the gap that there is no detection method for enrofloxacin metabolites in sea cucumbers in my country at present, and provides a new reference idea for researching metabolites of other drugs and screening unknown drugs.

Description

technical field [0001] The invention belongs to the technical field of aquatic product detection, and relates to a method for detecting enrofloxacin metabolites in sea cucumbers by using high performance liquid chromatography-tandem mass spectrometry-ion trap mass spectrometry (LC-MS / MS-Qtrap). Background technique [0002] Enrofloxacin (ENR) belongs to the third generation of quinolone synthetic antibacterial drugs and is one of the main antibacterial drugs in aquaculture. The connection function interferes with the synthesis of DNA, RNA and protein, thereby achieving the purpose of antibacterial and bactericidal. However, the non-standard use and abuse of enrofloxacin can be found in animals, food, [0003] Bacterial resistance increases in the environment and in consumers, posing a serious threat to human and animal health. [0004] Enrofloxacin has different degradation or metabolism pathways in the environment and in animals, and its degradation products in the enviro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/62G01N30/02
Inventor 邢丽红孙伟红李兆新翟毓秀郭江涛郭萌萌吴海燕唐锡招
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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