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Culture medium for culturing orchid tissue and culture method thereof

A technology of tissue culture and culture medium, applied in horticultural methods, botany equipment and methods, angiosperms/flowering plants, etc., can solve the problems of inconsistent growth of seedlings, long time for slow seedlings, low seedling rate, etc. The effect of more leaves, faster seedling emergence, and increasing the percentage of large seedlings

Active Publication Date: 2014-04-30
海宁市袁花镇工业投资有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing medium often has defects such as low seedling rate, inconsistent growth of seedlings, and long time for slowing down seedlings, and it is usually necessary to add expensive coconut juice as a nutrient in the medium, and the cost is relatively high

Method used

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  • Culture medium for culturing orchid tissue and culture method thereof
  • Culture medium for culturing orchid tissue and culture method thereof
  • Culture medium for culturing orchid tissue and culture method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment one: prepare the culture medium of orchid tissue culture of the present invention

[0025] According to the formulation in Table 1 below, each component of 1 / 2 MS medium was weighed, wherein the amount of macroelements was half of that of standard MS medium.

[0026] Table 11 / 2 Composition and dosage of MS medium

[0027]

[0028]

[0029] 1. Preparation of Phalaenopsis tissue culture medium

[0030] According to the formula in Table 2 below, each component of the Phalaenopsis tissue culture medium was weighed.

[0031] Table 2 Phalaenopsis tissue culture medium composition and dosage

[0032] Sample serial number

basal medium

agar

White sugar

Chitosan

cucumber juice

activated carbon

Medium No. 1

1 / 2MS medium

7g

15g

0.5g

100g

1g

Medium No. 2

1 / 2MS medium

8g

18g

1g

150g

2g

medium 3

1 / 2MS medium

9g

20g

1.5g

200g

2.5g

Med...

Embodiment 2

[0045] Embodiment two: Phalaenopsis tissue culture

[0046] 1. Adventitious bud induction: take robust and mature pedicels as explants, inoculate them in Phalaenopsis induction medium after disinfection (with 1 / 2MS as the basal medium, 5 mg of 6-benzylamino glands are added to each liter of 1 / 2MS medium purine, 0.5mg naphthaleneacetic acid and 100mL coconut water), after 25-35 days of culture, adventitious buds were induced from the pedicels;

[0047] 2. Inoculate adventitious buds in the Phalaenopsis proliferation medium (using MS as the basal medium, adding 8mg6-benzylaminoadenine, 0.5mg naphthaleneacetic acid and 100mL coconut water per liter of MS medium) for expansion culture, culture After 30 to 35 days, a large number of adventitious buds were obtained;

[0048] 3. With a sterilized scalpel, the adventitious buds are divided into small buds with 2 to 3 buds, and the small buds are inoculated in the Phalaenopsis tissue culture medium prepared in Example 1 to cultivate s...

Embodiment 3

[0056] Embodiment three: Cymbidium tissue culture

[0057] 1. Take healthy newborn axillary buds free from diseases and insect pests as explants, inoculate them in Cymbidium inoculation medium after disinfection (using MS as the base medium, adding 1.5mg of 6-benzylaminoadenine, 0.2 mg naphthalene acetic acid and 100g banana puree), after 25-30 days of cultivation, protocorms were induced;

[0058] 2. Inoculate the protocorm in the Cymbidium multiplication medium (based on MS, supplemented with 1.5mg of 6-benzylaminoadenine, 0.5mg of naphthaleneacetic acid and 100g of banana puree per liter of MS medium) for expansion Cultivate and obtain a large number of protocorms after 28 to 32 days of cultivation;

[0059] 3. Inoculate the protocorms in the Cymbidium phalanx tissue culture medium prepared in Example 1 for strong seedling cultivation, and after 28 to 35 days of cultivation, obtain Cymbidium cymbidium seedlings reaching the emergence standard.

[0060] The culture conditi...

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Abstract

The invention discloses a culture medium for culturing orchid tissue. According to the culture medium, 1 / 2MS culture medium is taken as a basal culture medium, and agar, white granulated sugar, chitosan, cucumber juice and activated carbon are added. The culture medium disclosed by the invention has the advantages of low cost, high seedling rate, short seeding strengthening time and the like.

Description

technical field [0001] The invention relates to a medium for orchid tissue culture, and also relates to a method for orchid tissue culture. Background technique [0002] Orchid is a famous traditional Chinese flower. Because of its unique leaves, flowers and fragrance, it has a very high ornamental value. In recent years, orchid varieties such as Phalaenopsis and Cymbidium have also become popular high-end indoor potted flowers in the flower market in my country. Phalaenopsis B1.Spp. is named because of its large flowers, long flowering period, bright colors, rich colors, beautiful and unique flower shapes, dancing like butterflies, and is known as the "Queen of Orchids" among tropical orchids. Come one of the most popular orchids. Cymbidium is a variety bred by artificial hybridization of large-flowered epiphytic species, small-flowered vertical species and some ground orchids through more than one hundred years of multi-generation artificial hybridization in the genus. ...

Claims

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Application Information

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IPC IPC(8): C05G3/00A01H4/00A01H6/62A01H5/02
CPCA01H5/02C05F11/00A01H6/62A01H4/002C05G5/23
Inventor 陈永得
Owner 海宁市袁花镇工业投资有限公司
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