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Method for building nucleotide formula to identify plant variety by using ribosome deoxyribonucleic acid (DNA)

A technology of nucleotide molecular formula and plant variety, which is applied in biochemical equipment and methods, and the determination/inspection of microorganisms, etc., can solve the problems of the same name of foreign objects, unclear source records, and restrictions on the production, promotion and breeding of crape myrtle varieties.

Inactive Publication Date: 2014-04-30
INST OF BOTANY CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the lack of sorting of variety resources, the production, promotion and breeding of Lagerstroemia indica varieties are limited.
The sources of traditional crape myrtle varieties are not well documented, and there are phenomena of the same thing with different names and different names with the same name, etc., which lead to irregularities in the market and affect the normal trade of crape myrtle flowers and trees and the growth of the economic scale of related industries.
The lack of morphological markers and DNA molecular markers and the lack of a reliable identification technology system seriously restrict the genetic evaluation, protection and utilization of Lagerstroemia germplasm resources

Method used

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  • Method for building nucleotide formula to identify plant variety by using ribosome deoxyribonucleic acid (DNA)
  • Method for building nucleotide formula to identify plant variety by using ribosome deoxyribonucleic acid (DNA)
  • Method for building nucleotide formula to identify plant variety by using ribosome deoxyribonucleic acid (DNA)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0113] Embodiment 1, the establishment and application of the nucleotide molecular formula identification method of Lagerstroemia indica varieties

[0114] In order to construct a nucleotide molecular formula that can be used to effectively identify crape myrtle varieties, the inventors of the present invention obtained a DNA sequence containing a nuclear ribosomal DNA internal transcriptional spacer (ITS region) and part of the 26SrRNA gene from the following series of crape myrtle varieties, And the polymorphic sites among them were analyzed statistically. There are 10 kinds of crape myrtle varieties used as test materials, as follows: 'Honghuan Zhizhizi', 'Green Claw Red Butterfly', 'Bai Mixiang', 'Xantao Powder Crystal', 'Crinkle Petal Viola', 'Jianmin Red', 'Straight Calyx Red Claw Fen', 'Baoqing Purple', 'Long Claw Coral Viola' and 'Great Flower Viola Viola'.

[0115] 1. Primer design

[0116] According to the conserved regions at both ends of a region containing the I...

Embodiment 2

[0172] Embodiment 2, identify other crape myrtle samples

[0173] In order to verify the specificity of the nucleotide molecular formula established in Example 1 to the Lagerstroemia indica plant material, the inventors of the present invention also collected the leaves of Lagerstroemia indica, and extracted its genomic DNA according to the method in Example 1, and used sequence 1 and The primer pair composed of two single-stranded DNA molecules shown in sequence 2 was amplified by PCR and sequenced, and the zw_ITS sequence of Lagerstroemia indica was obtained (sequence 13, compared with the zw_ITS sequences of 10 crape myrtle varieties described in Example 1 , it was found that the 108th nucleotide of Lagerstroemia nadina was missing). Put together the ITS sequence of Lagerstroemia indica and the sequences of the aforementioned 10 varieties of Lagerstroemia indica and compare them using software (such as Mega5 or ClustalX) to obtain a sequence alignment matrix (alignment), an...

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Abstract

The invention discloses a method for identifying plant variety, and provides a method for identifying crape myrtle variety. The method comprises the following steps: 1) carrying out polymerase chain reaction (PCR) amplification by taking a genome deoxyribonucleic acid (DNA) of to-be-detected crape myrtle as a template and using a primer composed of two single-chain DNA molecules shown in a sequence 1 and a sequence 2, so as to obtain a PCR product; 2) sequencing the PCR product, and listing the nucleotide formulas of the to-be-detected crape myrtle according to the sequencing result; 3) comparing the nucleotide formulas obtained in the step 2) with corresponding nucleotide formulas of a plurality of crape myrtle varieties, so as to determine the variety of the to-be-detected crape myrtle. An experiment proves that the method is accurate, and simple and fast to operate, the production efficiency and the quality monitoring level of related enterprises can be improved, and the defects of the traditional method are effectively compensated. In addition, identification of a non-flowering phase (for example, a seedling stage) is simultaneously achieved. The method has important significance on facilitation of development of new variety breeding of the crape myrtle, crape myrtle flower and wood industries and urban garden greening, and environmental protection.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for identifying or assisting in identifying plant varieties, in particular to a method for identifying plant varieties by using ribosomal DNA sequences to construct nucleotide molecular formulas. Background technique [0002] Plant varieties refer to cultivated plants that are formed through artificial breeding with basically the same species, relatively stable heredity, and certain economic traits that humans need: such as ornamental traits, medicinal traits, or other economic traits, and are used as special means of production. group. Such as peony varieties, walnut varieties, apple varieties, crape myrtle varieties, etc. [0003] Plant varieties are generally identified and identified using phenotypic traits. However, there are seasonal differences in the performance of plant varieties, and in some seasons, there may be no traits that differ between varieties. For exampl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6858C12Q1/6869C12Q2531/113
Inventor 索志立黄建民侯伯鑫
Owner INST OF BOTANY CHINESE ACAD OF SCI
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