Quick sensitive detection method of potato viruses

Abstract

The invention discloses a method for potato Y virus, potato X virus, potato A virus and potato leaf roll virus in Yunnan potato main culture species Li potato No.6, Yun potato 401 and Xuan potato No.2 infected plants. The specific mixed antibody is utilized to entrap the potato Y virus, potato X virus, potato A virus and potato leaf roll virus in Yunnan potato main culture species Li potato No.6, Yun potato 401 and Xuan potato No.2 infected plants, so that the total RNA (ribonucleic acid) of the sample does not need to be extracted to perform random primer reverse transcription and specific virus primer compound PCR (polymerase chain reaction) reaction. The method combines quick specific immune precipitation and sensitive compound RT-PCR (reverse transcription-polymerase chain reaction), sets healthy potato random primer cDNA (complementary deoxyribonucleic acid) product and corresponding virus PCR specific segment positive plasmid as negative and positive controls, and thus, is quickly, accurately and sensitively used in detecting field potato plant samples, antiviral materials, field environment suspect infection sources and the like.

Description

technical field [0001] The invention belongs to the technical field of plant virology detection, and in particular relates to a rapid and sensitive detection method of potato virus. Background technique [0002] The field symptoms of potato virus disease are complex and diverse, and the common symptom types can be summarized as follows: (1) mosaic type. Light green, yellow-green and dark green mottled mottled leaves appear on the leaves (there are light mottled, heavy mosaic, shrunken mottled and macular mottled), and the leaves basically remain the same, or the leaves become smaller and wrinkled Shrinkage, dwarfing of plants. (2) Curly leaf type. Leaf margins curl upwards, even cylindrical, pale in color, hardened and leathery, and sometimes purplish red appears on the back of the leaves. (3) Necrotic type (or streak type). Leaf veins, petioles, stems and branches appear brown necrotic spots or conjoined streaks, and even leaves wither, die or fall off. (4) Arbuscular ...

AI Technical Summary

Problems solved by technology

[0008] During potato growth, the occurrence of potato virus diseases is common and seriously affects the yield and quality of potatoes. The main pathogens are potato virus Y, potato virus S, potato virus A and potato leafroll virus. Therefore, it is impossible to formulate effective disease prevention measures; in the study of disease infection cycle and early disease occurrence, when using serological detection methods for detection, the detection sensitivity cannot better determine the infection of different viruses. Therefore, it is necessary to establish a A large, rapid, accurate and sensitive detection method for detection of virus transmission mediators, intermediate hosts, etc. in disease samples in different periods and in the surrounding environment of the field

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