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A kind of extraction method of dendrobium officinale polysaccharide

A technique for polysaccharides and extraction methods of Dendrobium candidum, which is applied in the field of processing Chinese herbal medicines, can solve the problems of high cost, low polysaccharide extraction rate, and low extraction rate, and achieve the effects of easy large-scale production, improved extraction rate, and simple operation

Active Publication Date: 2016-06-01
北京云尚时代科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the existing method for extracting polysaccharides from Dendrobium officinale, there are shortcomings such as low extraction rate and high impurity content in the extracted polysaccharides
For example, the Chinese invention patent "A Method for Separation and Purification of Dendrobium Polysaccharides" whose patent application number is 201110365323.7 uses a chloroform:n-butanol mixture with a volume ratio of 5:1 to repeatedly deproteinize the protein. The removal of polysaccharides leads to a decrease in the extraction rate of polysaccharides
It also has the disadvantages of complicated production process and high cost.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Take fresh Dendrobium officinale, chop it up, put it in -25 ℃ and freeze for 7 hours, after thawing at room temperature, soak it with 10 times the weight of absolute ethanol, remove the soaking liquid, and get the medicine dregs; Add 0.5% sodium hydroxide solution at 1:20 and decoct. The number of decoctions is 3 times, and the decoction time is 4 hours each time. The extracts are combined and concentrated at normal pressure to the original 1 / 2 of the volume to obtain a concentrated solution; adjust the pH value of the concentrated solution to 10, let it stand for 48 hours, and filter to obtain the filtrate I; add the filtrate I to activated carbon, filter to obtain the filtrate II, and dry , to obtain the polysaccharide of Dendrobium officinale. Wherein, the weight that activated carbon adds is 1% of described filtrate I weight.

[0028] After testing, the extraction rate of Dendrobium polysaccharide is 37%, and its polysaccharide content is 97%. The detection of its...

Embodiment 2

[0030] Take fresh Dendrobium officinale, chop it up, put it in -20 ℃ and freeze for 8 hours, after thawing at room temperature, soak it with 20 times the weight of ethanol solution with a mass concentration of 75%, remove the soaking liquid, and obtain medicinal residues; press the medicinal residues The weight ratio of solid to liquid is 1:40, adding sodium hydroxide solution with a weight content of 0.5% for decoction, the number of decoction is 1 time, and the time of each decoction is 6 hours to obtain the extract, and the extract is decompressed Concentrate to 1 / 2 of the original volume to obtain a concentrated solution; adjust the pH value of the concentrated solution to 11, let it stand for 24 hours, and filter to obtain the filtrate I; add the filtrate I to activated carbon, filter to obtain the filtrate II, dry, That is, the dendrobium officinale polysaccharide was obtained. Wherein, the weight that gac adds is 0.5% of described filtrate I weight.

[0031] After test...

Embodiment 3

[0033] Take fresh Dendrobium officinale, chop it up, put it in -22 ℃ and freeze for 6 hours, after thawing at room temperature, soak it with 8 times the weight of ethanol solution with a mass concentration of 95%, remove the soaking liquid, and obtain medicinal residues; press the medicinal residues The solid-liquid weight ratio is 1:10, and the potassium hydroxide solution with a weight content of 0.5% is added for soaking. The number of soaking times is 3 times, and the time of each soaking is 2 hours. The solution was concentrated under reduced pressure to 1 / 2 of the original volume to obtain a concentrated solution; the concentrated solution was adjusted to pH 9, allowed to stand for 36 hours, and filtered to obtain the filtrate I; the filtrate I was added to activated carbon and filtered to obtain the filtrate II , and dried to obtain the polysaccharide of Dendrobium officinale. Wherein, the weight that gac adds is 0.8% of described filtrate I weight.

[0034] After test...

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PUM

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Abstract

The invention relates to an extracting method of dendrobium officinale polysaccharides, and belongs to the technical field of processing of Chinese herbal medicines. The extracting method comprises the following steps of: a, soaking, namely, cutting up fresh dendrobium officinale, freezing for 6 to 8 hours under a temperature of -25 to -20 DEG C, then unfreezing under normal temperature, soaking with an organic solvent which is 8 to 20 times by weight, and then removing the soaking liquid to obtain the residues; b, extracting with alkali liquor, namely, adding 0.5 to 1wt% alkali liquor to the residues based on the weight ratio of solid to liquor of 1: 10 to 40 for extracting to obtain extract liquor; c, concentrating, namely, concentrating the extract liquor to be 1 / 2 of the original volume, thus obtaining concentrated liquor; d, adjusting pH of the concentrated liquor to be 9 to 11, standing, and filtering to obtain the filtrate I; e, adding activated carbon to the filtrate I, filtering to obtain filtrate II, and then drying to obtain dendrobium officinale polysaccharides. The extracting method has the beneficial effects that the extracting rate of polysaccharides is obviously increased, and the obtained polysaccharides extract is high in polysaccharides content.

Description

technical field [0001] The invention relates to the technical field of processing Chinese medicinal materials, in particular to a method for extracting dendrobium officinale polysaccharides. Background technique [0002] Dendrobium officinale is the stem of Dendrobium officinale, also known as black knot grass. The medicinal use was first recorded in "Shen Nong's Materia Medica", and it was listed as the top grade. It has the effects of nourishing the five viscera, strengthening the yin, thickening the stomach, lightening the body and prolonging life. Afterwards, all herbal medicines have been recorded in successive dynasties. The 2010 edition of "The Pharmacopoeia of the People's Republic of China" (Part One) lists it separately, and contains: sweet, slightly cold. Return stomach, kidney channel. It has the effects of nourishing the stomach and promoting body fluid, nourishing yin and clearing away heat. It is used for yin deficiency and body fluid deficiency, dry mouth...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00
Inventor 黄显勇余鸿飞
Owner 北京云尚时代科技有限公司