Determination method of inclusion compound inclusion rate tester
A measurement method and a technology of inclusion rate, applied in the direction of material excitation analysis, fluorescence/phosphorescence, etc., can solve the problems of complex operation, large sample consumption, high sample consumption, etc., and achieve the effect of high sensitivity, low sample volume and simple operation
Inactive Publication Date: 2016-01-06
ZHEJIANG FORESTRY UNIVERSITY
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Problems solved by technology
[0003] At present, the determination of the inclusion rate of drug inclusion complexes is usually carried out directly by spectroscopic method, that is, it is calculated according to the changes in the spectral characteristics of drug molecules before and after inclusion. This method cannot measure drug inclusion complex molecules without spectral characteristics, and the operation It is complex, the sample amount is high, and there are certain defects.
[0004] After the drug enters the human body, it can be transported to the body through body fluids, which can reduce the fluorescence quantum yield of the protein with endogenous fluorescence (fluorescence quenching), and the drug inclusion complex can slowly release the drug through the body fluid transport to the target site, which also has a certain effect on the protein. Fluorescence quenching effect, so the inclusion rate of drug inclusion complexes can be calculated according to the difference in fluorescence quenching of proteins. This method has not been reported. The fluorometer involved is an important instrument, and most traditional fluorometers require A large beam is used to excite the sample, and a large-aperture light converging device is required to improve the sensitivity of the instrument, so the instrument is huge and the sample consumption is large. CN203299124U has announced a portable fluorometer, which uses a lens to converge the excitation light, and passes through a spatial filter or The baffle removes the background and stray light, which greatly improves the sensitivity of the instrument. On this basis, this application designs a novel and simple clathrate inclusion rate measuring instrument and its test methods.
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[0021] Below the present invention will be described in further detail in conjunction with embodiment and with reference to accompanying drawing, see figure 1 and figure 2 , the clathrate inclusion rate tester, functionally, includes a detection part (1), a control part (2) and a display part (3), the detection part (1) includes a sample tube (11) in the center, The right side of the horizontal direction is provided with a first lens (12) and a second lens (14), and a short-wave filter (13) is arranged between the first lens (12) and the second lens (14). An excitation light source (15) is arranged on the right side of the lens (14), a third lens (16) and a fourth lens (18) are arranged directly above the sample tube (11), and the third lens (16) and the fourth lens (18) ) is provided with a long-wave pass filter (17), and the upper end of the fourth lens (18) is provided with a photomultiplier tube (19); the control part (2) is a chip made by self-programming according to t...
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The invention discloses a measurement method of an inclusion rate measurer for an inclusion compound. The measurer comprises a detection part (1), a control part (2) and a display part (3), wherein the detection part (1) comprises a sample pipe (11), a first lens (12), a short-wave pass filter (13), a second lens (14), an excitation light source (15), a third lens (16), a long-wave pass filter (17), a fourth lens (18) and a photoelectric multiplier tube (19); the control part (2) is mainly a chip; the display part (3) is a liquid crystal display screen. The method for measuring the inclusion rate of the inclusion compound by adopting the measurer comprises the five steps of 1, preparing materials; 2, preparing a solution; 3, correcting the measurer; 4, constructing a fluorescence intensity-inclusion rate standard curve; and 5, measuring the inclusion rate of a sample. According to the measurement method, a new way for measuring the inclusion compound without a spectral feature by adopting a fluorescence method is created; furthermore, the improved measurer is high in sensitivity, low in sample amount, easy to operate and large in application range.
Description
technical field [0001] The invention relates to a method for using a chemical experiment instrument, in particular to a method for measuring the inclusion rate of a drug inclusion compound, that is, the inclusion rate of a β-cyclodextrin-drug inclusion compound. Background technique [0002] Cyclodextrins are a type of cyclic oligosaccharides formed by connecting 6 to 8 glucose units through 1,4 glycosidic bonds. They have the properties of "hydrophilic on the outside, hydrophobic on the inside, and special cavity". Molecular interactions form host-guest inclusion complexes with many organic or inorganic molecules, which have been widely used in the field of supramolecular chemistry. β-cyclodextrin is the most widely used in the category of cyclodextrins. It is a class composed of 7 glucose A class of cyclic oligosaccharides formed by interconnecting units through 1, 4 glycosidic bonds, which can contain hydrophobic guest drug molecules of various shapes and sizes that match...
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IPC IPC(8): G01N21/64
Inventor郭明伍周玲詹敏忠
OwnerZHEJIANG FORESTRY UNIVERSITY