Determination method of inclusion compound inclusion rate tester

Abstract

The invention discloses a measurement method of an inclusion rate measurer for an inclusion compound. The measurer comprises a detection part (1), a control part (2) and a display part (3), wherein the detection part (1) comprises a sample pipe (11), a first lens (12), a short-wave pass filter (13), a second lens (14), an excitation light source (15), a third lens (16), a long-wave pass filter (17), a fourth lens (18) and a photoelectric multiplier tube (19); the control part (2) is mainly a chip; the display part (3) is a liquid crystal display screen. The method for measuring the inclusion rate of the inclusion compound by adopting the measurer comprises the five steps of 1, preparing materials; 2, preparing a solution; 3, correcting the measurer; 4, constructing a fluorescence intensity-inclusion rate standard curve; and 5, measuring the inclusion rate of a sample. According to the measurement method, a new way for measuring the inclusion compound without a spectral feature by adopting a fluorescence method is created; furthermore, the improved measurer is high in sensitivity, low in sample amount, easy to operate and large in application range.

Description

technical field [0001] The invention relates to a method for using a chemical experiment instrument, in particular to a method for measuring the inclusion rate of a drug inclusion compound, that is, the inclusion rate of a β-cyclodextrin-drug inclusion compound. Background technique [0002] Cyclodextrins are a type of cyclic oligosaccharides formed by connecting 6 to 8 glucose units through 1,4 glycosidic bonds. They have the properties of "hydrophilic on the outside, hydrophobic on the inside, and special cavity". Molecular interactions form host-guest inclusion complexes with many organic or inorganic molecules, which have been widely used in the field of supramolecular chemistry. β-cyclodextrin is the most widely used in the category of cyclodextrins. It is a class composed of 7 glucose A class of cyclic oligosaccharides formed by interconnecting units through 1, 4 glycosidic bonds, which can contain hydrophobic guest drug molecules of various shapes and sizes that match...

AI Technical Summary

Problems solved by technology

[0003] At present, the determination of the inclusion rate of drug inclusion complexes is usually carried out directly by spectroscopic method, that is, it is calculated according to the changes in the spectral characteristics of drug molecules before and after inclusion. This method cannot measure drug inclusion complex molecules without spectral characteristics, and the operation It is complex, the sample amount is high, and there are certain defects.

[0004] After the drug enters the human body, it can be transported to the body through body fluids, which can reduce the fluorescence quantum yield of the protein with endogenous fluorescence (fluorescence quenching), and the drug inclusion complex can slowly release the drug through the body fluid transport to the target site, which also has a certain effect on the protein. Fluorescence quenching effect, so the inclusion rate of drug inclusion complexes can be calculated according to the difference in fluorescence quenching of proteins. This method has not been reported. The fluorometer involved is an important instrument, and most traditional fluorometers require A large beam is used to excite the sample, and a large-aperture light converging device is required to improve the sensitivity of the instrument, so the instrument is huge and the sample consumption is large. CN203299124U has announced a portable fluorometer, which uses a lens to converge the excitation light, and passes through a spatial filter or The baffle removes the background and stray light, which greatly improves the sensitivity of the instrument. On this basis, this application designs a novel and simple clathrate inclusion rate measuring instrument and its test methods.

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