Method for preparing geniposide in eucommia leaf

[0024] Example 1. See the instructions attached figure 1 , Take 10Kg of dried Eucommia leaves, pulverize and pass through 80 mesh sieve, add 120Kg of water, adjust the temperature to 55℃, keep the temperature constant and adjust the pH to 4.5, then add 10g of cellulase, extract 60min under these conditions, filter, filter the residue again Extract for the second time according to the aforementioned extraction conditions, combine the two filtrates, and routinely filter. The extraction rate of geniposide is 95.8%; the operating conditions are P=0.3Mpa, T=45℃, PH=4.5, Q=70L/min The filtrate was passed through a 0.03μm ultrafiltration membrane, the extract was concentrated 11.2 times under this condition, and the average flux of the ultrafiltration membrane was 226.3L/m 2 ·H; Under the operating conditions P=1.5Mpa, T=50℃, PH=4.5, Q=20L/min, the ultrafiltration filtrate is then passed through a 200 molecular weight nanofiltration membrane, the ultrafiltration filtrate is concentrated 16.6 times under this condition, inorganic The salt ion removal rate is 63%, and the average flux of the nanofiltration membrane is 18.7L/m 2 ·H, the COD of the nanofiltration filtrate is 94; the nanofiltration concentrate is purified by D301R resin, first eluting impurities with 2 times the volume of the resin column, and then eluting with 2.5 times the volume of the resin at a concentration of 70% ethanol After collecting the ethanol eluates under reduced pressure and vacuum drying (the temperature of vacuum concentration and vacuum drying are not higher than 60℃), geniposide powder can be obtained. The purity of geniposide is 82.5% by HPLC. The rate is 1.35%.

[0025] Example 2: See the attachment figure 1 , Take 10Kg of dried eucommia leaves, pulverize and pass through an 80-mesh sieve, add 120Kg of water, adjust the temperature to 50℃, keep the temperature constant and adjust the pH to 4.5, then add 10g of cellulase, extract 80min under these conditions, filter, and filter the residue again Extract for the second time according to the aforementioned extraction conditions, combine the two filtrates, and routinely filter. The extraction rate of geniposide is 95.3%; the operating conditions are P=0.3Mpa, T=45℃, PH=4.5, Q=70L/min The filtrate was passed through a 0.02μm ultrafiltration membrane, and the extract was concentrated 12.8 times under this condition. The average flux of the ultrafiltration membrane was 221.3L/m 2 ·H; Under the operating conditions P=1.5Mpa, T=50℃, PH=4.5, Q=20L/min, the ultrafiltration filtrate is then concentrated through a 400 molecular weight nanofiltration membrane, and the ultrafiltration membrane filtrate is concentrated 15.2 times under these conditions , The removal rate of inorganic salt ions is 60%, and the average flux of the nanofiltration membrane is 19.5L/m 2 ·H, the COD of the nanofiltration filtrate is 91; the nanofiltration concentrate is purified by H103 resin, first eluting impurities with 2 times the volume of the resin column, and then eluting with 2.5 times the volume of the resin at a concentration of 70% ethanol After collecting the ethanol eluate under reduced pressure and vacuum drying (the temperature of vacuum concentration and vacuum drying are not higher than 60℃), geniposide powder can be obtained. The purity of geniposide is 83.6% by HPLC. The rate is 1.21%.