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Method for extracting ecdysone from spinacia oleracea

A technology for ecdysone and spinach, which is applied in the field of extraction and separation of plant-derived insect ecdysone analogs, can solve the problem of not extracting ecdysone and the like, and achieves the effects of reducing extraction cost, short time consumption and high extraction efficiency.

Inactive Publication Date: 2014-05-14
QINGDAO AGRI UNIV
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Problems solved by technology

There are many patents and reports on extracting ecdysone from Dewweed and Grass sinensis, but there is currently no technical system for extracting ecdysone (beta-Ecdysone) from spinach (Spinacia oleracea L.) and determining its content

Method used

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Embodiment Construction

[0016] The technical solutions of the present invention will be further described in detail below in conjunction with specific embodiments.

[0017] The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.

[0018] (1) Take spinach leaves, add liquid nitrogen, and grind them into powder.

[0019] (2) Put the powder in a 50ml centrifuge tube, add MeOH-CHCl 3 (9:1, V / V) to 50ml, placed in a constant temperature culture shaker and shaken for 2h, the temperature of the shaker was set at 37°C, and the rotation speed was set at 180rpm.

[0020] (3) Add diatomaceous earth 545 into the Buchner funnel, and use the circulating water type multi-purpose vacuum pump SHK-III to filter the sample.

[0021] (4) Pour the filtered sample into a separatory funnel, add 50ml of Hexane (Hexane) and a little NaCl, shake vigorously to make it fully extracted, and then let it stand for 30 minutes. After 30 minutes, remove the extra...

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Abstract

The invention discloses a method for extracting ecdysone from spinacia oleracea. The method comprises the following steps: taking a to-be-detected sample; grinding liquid nitrogen into powder and adding a mixed solution of MeOH-CHCl3 in a volume ratio of 9:150ml or 100% MeOH solution until the solution is 50ml; vibrating at 37 DEG C for 2 hours and filtering by using silica gel powder; adding less NaCl and 50ml of n-Hexane to extract for two times; collecting an MeOH layer and drying by using a rotary evaporator; dissolving the sample again by using isopropanol; filtering and carrying out high performance liquid chromatography; determining the content. According to the method, an extract is subjected to the high performance liquid chromatography; the extraction speed is rapid and the extraction efficiency is high; the trace extraction of the extract can be realized.

Description

technical field [0001] The invention belongs to the technical field of extraction and separation of plant-derived insect ecdysone analogues, and relates to a method for extracting ecdysone from spinach. Background technique [0002] Phytoecdysone 1 is a very important class of plant secondary metabolites and a natural steroidal compound with insect molting activity. Phyto-derived ecdysone can promote the molt of insects, and high concentration of ecdysone can interfere with the normal physiological metabolism of insects, causing insects to molt early and become weak or deformed individuals. Insects are quite sensitive to ecdysone, and mild cases can cause insect sterility, and severe cases can lead to death. Therefore, phytoecdysteroids play an important role in plant defense against non-adaptive phytophagous insects, and very low concentration levels can prevent pest attack. Extracting ecdysteroids from plants has become a hot spot in production, research and development....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J9/00G01N30/02
Inventor 段方猛宋雯雯
Owner QINGDAO AGRI UNIV
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