Stress-resistant strain h4-1l that assists host plants in alleviating drought stress

Abstract

The invention relates to a stress-resisting strain H4-1L for assisting host plants to relieve drought stress and belongs to the technical field of plant protection. The H4-1L strain is vibrated and cultured in an LB culture liquid at 28 DEG C at the speed of 180 rpm (revolutions per minute) for 12-16 hours, and then is centrifuged for 10 minutes at the speed of 6000rpm; the H4-1L strain is re-suspended by using sterilizing water and is diluted into a bacterium agent with the total live bacterium concentration of 1*10<9>-1*10<10>CFU / mL. Under a room-temperature condition, the 10<9>CFU / mL bacterium agent is used for carrying out root-irrigation treatment in a process of transplanting chilies and cucumbers and the use amount is 20mL per strain; the water is normally irrigated for 2 times in one week of transplanting; after one week, drought treatment is carried out. Compared with a control group, the strain H4-1L is used for treating and the drought-resisting capability of the chilies and the cucumbers can be improved; root lengths are increased by 30.8% and 25.3%; the fresh weights are increased by 26.6% and 44.4%; the chlorophyll contents are increased by 82.9% and 20.7%; the root activity is increased by 56.0% and 49.6%.

Description

technical field [0001] The invention belongs to the technical field of plant protection, and relates to a stress-resistant bacterial strain H4-1L capable of assisting host plants in alleviating drought stress. Background technique [0002] Plants experience drought stress when the rate of plant transpiration exceeds the rate of water uptake or when the soil is starved of water available to the plant. Drought stress is the most common crop growth stress in field conditions. According to statistics, the world's arid and semi-arid areas account for 1 / 3 of the earth's land area, and my country's arid and semi-arid areas account for about 1 / 2 of the country's land area. At the same time, other semi-humid or even humid areas often have periodic, seasonal or temporary droughts. Especially in 2011, the severe drought in five provinces in the middle and lower reaches of the Yangtze River in my country has reportedly caused 3,705.1 thousand hectares of crops to be affected, includin...

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Problems solved by technology

[0003] At present, under drought conditions, in addition to dispatching irrigation water within one's ability, people mainly rely on the following ways to alleviate the damage caused by drought to agricultural production: 1. Chemical regulation methods: some can promote water regulation, osmotic regulation, stomatal regulation and photosynthesis Chemical substances that regulate a series of physiological and biochemical mechanisms can enhance the metabolic activity of plants and improve their drought resistance, but no matter what kind of chemical regulators, there are economic costs and side effects on the environment and soil; two genetic engineering: in recent years, The use of genetic engineering technology to improve the level of drought-responsive gene products in plants has become a means of cultivating new stress-resistant varieties. So far, many plants have successfully introduced relevant drought-resistant genes and improved drought resistance, such as rice, tobacco, Cotton, etc., however, the use of genetic engineering to cultivate drought-resistant varieties in vegetables is less; in addition, in the long-term evolution process, plants themselves have also established responses to drought stress, but plants themselves regulate drought resistance. Limited, insufficient to cope with stress from persistent drought

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