Fermentation method for production of long-chain dicarboxylic acids

A long-chain dibasic acid and fermentation method technology, which is applied in the field of fermentation to produce long-chain dibasic acids, can solve the problems of prolonging the fermentation cycle, reducing biomass, and affecting the fermentation level, so as to promote the growth of bacteria and relieve foaming problems, the effect of increasing acid production levels

Active Publication Date: 2014-05-21
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Most of the defoamers used at this stage are polyether organic substances. The addition of such defoamers will, on the one hand, slow down the accumulation of microbial biomass and microbial activity. The biomass will be reduced by 10% to 20%; on the other hand, it will prolong the fermentation period and affect the fermentation level

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Medium preparation: sucrose 30g / L, corn steep liquor 1g / L, yeast extract 1g / L, sodium chloride 1g / L, potassium dihydrogen phosphate 5g / L, magnesium sulfate 1g / L, urea 2g / L.

[0024] (2) Culture of fermented seed liquid: Inoculate the slant-preserved strains into a 3000mL shake flask for strain activation cultivation. The volume of the activation culture medium is 500mL, the cultivation temperature is 32°C, the rotation speed of the shaker is 120rpm, and cultivation is carried out for 48 hours;

[0025] (3) Dibasic acid fermentation: Add 1000mL of the activated strain seed liquid to 6500mL fermentation medium, and add 500mL of dodecane. During the fermentation process, pH was adjusted with sodium hydroxide solution (30wt%). The initial pH value was set to 4.3, the stirring speed was set to 600 rpm, and the aeration was set to 7000 mL / min. 40 mL of glycerol was added for 24 hours, then the pH was adjusted to 7.2, and 1000 mL of dodecane was added. The pH value was r...

Embodiment 2

[0027] (1) Medium preparation: sucrose 40 g / L, corn steep liquor 1.5 g / L, yeast extract 1.5 g / L, sodium chloride 1 g / L, potassium dihydrogen phosphate 4 g / L, magnesium sulfate 1 g / L, urea 2 g / L.

[0028] (2) Culture of fermented seed liquid: Inoculate the slant-preserved strains into a 3000mL shake flask for strain activation cultivation. The volume of the activation culture medium is 500mL, the cultivation temperature is 32°C, the rotation speed of the shaker is 120rpm, and the cultivation is carried out for 48 hours.

[0029] (3) Dibasic acid fermentation: Add 1000mL of activated strain seed liquid to 6450mL fermentation medium, and add 550mL tridecane. During the fermentation process, the pH was adjusted with sodium hydroxide solution (10wt%). The initial pH value was set to 4.5, the stirring speed was set to 700rpm, and the aeration was set to 8000mL / min. 100 mL of glycerol was added for 24 hours, then the pH was adjusted to 7.2, and 1100 mL of tridecane was added. The...

Embodiment 3

[0031] (1) Medium preparation: sucrose 50g / L, corn steep liquor 3g / L, yeast extract 2g / L, sodium chloride 1.5 g / L, potassium dihydrogen phosphate 8g / L, magnesium sulfate 2g / L, urea 5g / L .

[0032] (2) Culture of fermented seed liquid: Inoculate the slant-preserved strains into a 5000 mL shake flask for strain activation cultivation. The volume of the activation culture medium is 900 mL, the cultivation temperature is 32°C, the rotation speed of the shaker is 180 rpm, and the cultivation is carried out for 48 hours.

[0033] (3) Dibasic acid fermentation: Add 1000mL of the activated strain seed liquid to 5400mL fermentation medium, and add 500mL hexadecane. Sodium hydroxide solution (10wt%) was used for pH adjustment during fermentation. The initial pH value was 4.3, the stirring speed was 700 rpm, and the ventilation was set to 7000 mL / min; 60 mL of glycerin was added in 24 hours, then the pH value was adjusted to 7.2, and 1000 mL of hexadecane was added. At 48 hours and 72 ...

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Abstract

The invention discloses a fermentation method for production of long-chain dicarboxylic acids. The method includes: subjecting a dicarboxylic acid strain to enlargement culture to obtain a seed solution, utilizing the seed solution to ferment n-alkanes to produce acid, taking glycerol as a defoaming agent in the fermentation process, adjusting the pH value of the system, and replenishing n-alkanes, removing mycoprotein at the end of fermentation, and carrying out separation and recovery to obtain the long-chain dicarboxylic acid product. By adding a proper defoamer, the method improves the fermentation efficiency and the quality of the dicarboxylic acid product.

Description

technical field [0001] The invention belongs to the field of biochemical industry, and mainly relates to a fermentation method for producing long-chain dibasic acids. Background technique [0002] Long chain dicarboxylic acids (Long chain dicarboxylic acids) refer to aliphatic dicarboxylic acids (DCn for short) containing more than 10 carbon atoms in the carbon chain, including saturated and unsaturated dicarboxylic acids. Fine chemical products. In the chemical industry, long-chain dibasic acids are used to synthesize high-grade spices, high-performance nylon engineering plastics, high-grade nylon hot-melt adhesives, high-temperature dielectrics, high-grade paints and coatings, high-grade lubricating oils, cold-resistant plasticizers, resins, medicines and pesticides important raw materials. [0003] At present, long-chain dibasic acids are mainly produced by microbial fermentation. Using the unique oxidation ability of microorganisms and the action of microbial intracell...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12R1/72C12R1/645C12R1/78C12R1/84
Inventor 张霖师文静廖莎李晓姝王领民
Owner CHINA PETROLEUM & CHEM CORP
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