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Use of micromolecule rna-508-5p as an antitumor marker

A technology with usage and function, applied in the field of anti-tumor drug development, can solve the problems of unclear research on the regulation mechanism, proliferation and apoptosis process, and achieve the effect of inhibiting invasion and promoting apoptosis

Inactive Publication Date: 2017-01-11
NINGXIA MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Among numerous miRNAs, miR-508 has been confirmed to be abnormally down-regulated in gastric cancer, liver cancer, kidney cancer and ovarian serous carcinoma, and is likely to participate in the process of tumors and become a potential tumor suppressor gene. Currently, miR-508 The regulation mechanism of -5p in gastric cancer, liver cancer and other tumor cells and its influence on the proliferation and apoptosis process are still unclear. Therefore, the development of miR-508-5p as a strategy for the prevention, diagnosis and treatment of gastric and liver cancer The basic research of treatment and the research of antitumor drugs have important significance and application prospects

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  • Use of micromolecule rna-508-5p as an antitumor marker
  • Use of micromolecule rna-508-5p as an antitumor marker
  • Use of micromolecule rna-508-5p as an antitumor marker

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Embodiment Construction

[0020] 1. The expression of the oncogene SKP2 gene was detected by immunohistochemical method. From February 2013 to August 2013 in the Department of Pathology of Ningxia Medical University General Hospital, 12 formalin-fixed and paraffin-embedded tissues (FFPE) of patients with moderately and poorly differentiated gastric adenocarcinoma and formalin-fixed tissue from patients with normal gastric mucosa were collected. 10 cases of fixed paraffin-embedded tissues (FFPE), serial sections, 4um thick, dewaxed and hydrated, 1mM EDTA (PH9.0) antigen retrieval, H2O2 to eliminate endogenous peroxidase, 10% goat serum to block , the mouse anti-human SKP2 primary antibody was incubated overnight at 4°C, and the goat anti-mouse SKP2 secondary antibody was incubated at 37°C for 1h. The DAB staining kit was used to stain the positive area, and the degree of staining was controlled under the microscope. Finally, hematoxylin counterstained, HCL-alcohol differentiation, ammonia water returned...

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Abstract

The invention provides an application of miRNA (Ribonucleic Acid)-508-5p to the control of metabolism of cell strains of liver cancer and gastric cancer, and particularly provides an application of the miRNA-508-5p expected as a marker for preventing and treating tumor. More and more evidences prove that miRNA can be used as a cancer gene or a cancer inhibiting gene to take part in the control for the generation and development processes of various cancers. An experiment for constructing a model for infecting a tumor cell by lentivirus proves that the miRNA-508-5p has a remarkable inhibiting effect on the proliferation, migration, invasion and the like of tumor cell strains HepG2 and SGC7901, is capable of promoting the cell apoptosis and has a cell cycle and metabolism regulating effect, so that the miRNA-508-5p is possible to become a brand new target spot for preventing and treating tumor; in addition, target gene deleting and selecting software and a dual-luciferase report system prove that the miRNA-508-5p takes a cell regulating effect through the expression of a specific targeted inhibition cancer gene Skp2 (Sphase Kinaseassociated Protein 2).

Description

technical field [0001] The present invention relates to a new application of endogenous non-coding small RNAs, in particular to the application of miR-508-5p as a new tumor marker in the prevention and treatment of gastric cancer and liver cancer and in the development of antitumor drugs. Background technique [0002] Gastric cancer is a common malignant tumor that endangers human health. Its case fatality rate ranks first among malignant tumors in my country. So far, the etiology is unknown and the clinical treatment effect is not good. Primary liver cancer is one of the most common malignant tumors in clinical practice. The global incidence rate is year by year. increase. Therefore, research on the pathogenesis and clinical treatment of gastric cancer and liver cancer is the fundamental way to reduce their harm. [0003] MicroRNA (miRNA) is a class of endogenous non-coding single-stranded RNA discovered in recent years, and its length is about 18-25 nucleotides. miRNA reg...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6886C12Q2600/158C12Q2600/178
Inventor 徐广贤魏军张爱君白静
Owner NINGXIA MEDICAL UNIV