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Micro RNA (Ribonucleic Acid) molecular marker for diagnosing rheumatoid arthritis and detection kit thereof

A detection kit and marker technology, applied in the field of biotechnology diagnosis, can solve problems such as disease prediction that cannot elucidate the pathogenesis, and achieve the effect of high specificity and sensitivity

Inactive Publication Date: 2014-05-21
焦志军 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, it is still impossible to elucidate the pathogenesis of RA and predict the disease, so it is very important to find biomarkers for early diagnosis of the disease and monitoring the course of the disease

Method used

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  • Micro RNA (Ribonucleic Acid) molecular marker for diagnosing rheumatoid arthritis and detection kit thereof
  • Micro RNA (Ribonucleic Acid) molecular marker for diagnosing rheumatoid arthritis and detection kit thereof
  • Micro RNA (Ribonucleic Acid) molecular marker for diagnosing rheumatoid arthritis and detection kit thereof

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Experimental program
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Embodiment Construction

[0022] The following describes the specific implementation of the above technical solution in conjunction with the accompanying drawings.

[0023] The overall experimental plan is as follows:

[0024] 2ml of peripheral blood was collected in EDTA-K from 80 RA patients and 75 normal healthy persons who were clinically diagnosed in the Affiliated Hospital of Jiangsu University. 2 Centrifuge the anticoagulant tube at 3000r / min for 10min at room temperature, transfer the upper plasma to a clean 1.5ml centrifuge tube, then centrifuge at 16000×g at 4°C for 10min, draw the upper plasma into a clean 1.5ml centrifuge tube, and freeze at -80°C for later use.

[0025] 1. Microarray screening group: 5 cases of clinically diagnosed RA patients with initial active stage were selected, and more than 1ml of plasma was collected from each of the 5 healthy persons for physical examination and sent to Shanghai Kangcheng Biotechnology using Exiqon Company to use miRCURY TM LNA Array (version 18...

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Abstract

The invention provides nine micro RNA (Ribonucleic Acid) (miRNA) molecular markers for diagnosing rheumatoid arthritis and an application thereof to a detection kit. The miRNA molecular markers have abnormal expression in blood of patients with the rheumatoid arthritis to diagnose the rheumatoid arthritis; the miRNA molecular marker has high combination sensitivity and specificity; materials are convenient to fetch; the miRNA molecular marker is safe and noninvasive and provides important evidences to early diagnosis of diseases and disease course monitoring. The invention further provides a kit for diagnosing the rheumatoid arthritis and the application of the miRNA molecular marker to the diagnosing kit. The kit is characterized by comprising the combination of the nine miRNA molecular markers for detecting blood plasma and a reagent for detecting the nine miRNA molecular markers.

Description

technical field [0001] The invention belongs to the field of biotechnology diagnosis, and relates to combinations of nine microRNA molecular markers for diagnosing rheumatoid arthritis and applications thereof. Background technique [0002] Rheumatoid arthritis (RA) is a chronic, systemic autoimmune disease, the main pathological changes of which are the obvious thickening of the synovial membrane and the infiltration of a large number of inflammatory cells, the formation of pannus and the erosion of cartilage tissue. destroy. For a long time, rheumatoid factor and anti-cyclic citrullinated protein antibody are serological indicators in the diagnostic criteria of the American College of Rheumatology, but the sensitivity and specificity are not high. At present, the pathogenesis of RA and the prediction of the disease cannot be clarified, so it is very important to find out the biomarkers for the early diagnosis of the disease and the monitoring of the course of the disease....

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/113
CPCC12Q1/6883C12Q2600/158C12Q2600/178
Inventor 焦志军王文红张莹莹朱波
Owner 焦志军
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