Pure-bacteria-inoculated liquid circular making method for Fujian monascus vinegar
A technology of liquid circulation and monascus vinegar, applied in the field of vinegar, can solve the problems of increased acetic acid content, long fermentation period, and decreased concentration of Acetobacter
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Embodiment 1
[0027] 1 Preparation of Fujian Monascus Vinegar Mother
[0028] 1.1 Cooking upland rice: Take 10kg of upland rice (mountain rice), wash it with water, add 25kg of water to soak for 15 hours; wash it 4 times with water, drain it; steam the upland rice in a steamer for 20 minutes, and spread it out to dry.
[0029] 1.2 Hydrolytic acidification of upland rice rice: Take 2kg of three-year mature vinegar containing 6% acetic acid and mix well with the dried upland rice rice, and let it rest for 12 hours; move the upland rice rice to the altar, add 30kg of three-year mature vinegar containing 6.5% acetic acid; cover, Cultivated at room temperature for 6 months, containing 6% acetic acid.
[0030] 1.3 Maturity of Fujian red koji vinegar mother vinegar: add 15kg of red koji wine with 18% ethanol content, continue to cultivate at room temperature for 1 year, and the acetic acid content reaches 6.5%; add 15 kg of Acetic acid up to 7%. During the whole cultivation period, open the alta...
Embodiment 2
[0052] 1 Preparation of Fujian Monascus Vinegar Mother
[0053] With embodiment 1.
[0054] 2 Preparation of red yeast rice wine
[0055] With embodiment 1.
[0056] 3 strain culture
[0057] The bacterial strain used is the B strain, and the B strain is the Y5072 strain of Gluconacetobacter stutzeri,
[0058] 3.1 For the cultivation of strains, pick a small amount of bacterial lawn from the slant of strain B and inoculate it into a 1500mL Erlenmeyer flask containing 500mL of acid-producing medium; Cell concentration reaches 2.0 x 10 9 / mL, that is, the bacterial liquid of strain B was obtained; the formula of the medium was as follows: glucose 3%, yeast powder 1%, absolute ethanol 5%, pH 6.0.
[0059] 3.2 Preparation of Acetobacter cell suspension: take 500mL of strain B bacteria solution, centrifuge at 5000r / min for 15min, pour off the supernatant, and suspend the cells with a mixture of 300mL Fujian red koji vinegar mother and 100mL red koji wine. B strain cell suspen...
Embodiment 3
[0069] 1 Preparation of Fujian Monascus Vinegar Mother
[0070] With embodiment 1.
[0071] 2 Preparation of red yeast rice wine
[0072] With embodiment 1.
[0073] 3 strain culture
[0074] The strains used are A strain and B strain.
[0075] 3.1 For the cultivation of strains, pick a small amount of lawn from the Acetobacter slant of strain A or strain B and inoculate them into a 1500mL Erlenmeyer flask containing 500mL of acid-producing medium; cultivate in a shaker at 30°C and 150r / min for 36h , so that the concentration of Acetobacter cells in the medium reached 3.0×10 9 / mL, that is to prepare the bacterial liquid of strain A or strain B respectively; the medium formula is as follows: glucose 2%, yeast powder 1%, absolute ethanol 5%, pH 5.5.
[0076] 3.2 Preparation of Acetobacter cell suspension: take 500mL of strain A or 500mL of strain B, centrifuge for 15min at a speed of 6000r / min, discard the supernatant, and use Fujian red yeast vinegar Suspend the cells in...
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