The invention discloses an anti-fatigue strawberry grape sparkling wine. The anti-fatigue strawberry grape sparkling wine is prepared from the following raw materials by weight part: 100-120 of wild strawberries, 50-60 of Cabernet Sauvignon, 6-8 of tomato, 1-2 of stevia rebaudiana, 1-2 of pandan leaf, 2-3 of Hibiscus manihot flower, 3-4 of pecan kernel, 1-2 of flammulina velutipes, 1-2 of Gynostemma pentaphyllum, a proper amount of macerating enzyme and aroma-producing active dry yeast, and a proper amount of red wine yeast and a compound clarifying agent. The anti-fatigue strawberry grape sparkling wine provided by the invention is added with a variety of raw materials with fatigue relieving efficacy, has rich and comprehensive nutrition, and significant health care effect, the finished product sparkling wine is ruby red or purple red, has bright red color, is clear and transparent, and full-bodied, when the wine is injected into a cup, the bubbles are rich, the taste is pure, the sweetness and sour are palatable, the taste is soft, the aftertaste is lingering, the strong fruity flavor and elegant wine aroma are integrated, the alcohol strength is 6-12%vol, and the carbon dioxide pressure is (20DEG C) 0.1-0.4MPa. Therefore, the anti-fatigue strawberry grape sparkling wine meets the demands of people for food safety, nutrition and health.