Method for preparing matrix of raw winter vegetables with municipal sludge
A technology of municipal sludge and matrix, applied in the biological field, can solve the problems of municipal sludge and achieve the effects of improving absorption and utilization, simple preparation process, and reducing heating and fermentation time
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[0033] The preparation method of the microbial starter described in step 3 includes the following steps:
[0034] Step 1. Preparation of soybean extract
[0035] (1) Wash the soybeans, dry them, smash them, and filter them with a screen with a diameter of 1 mm to obtain soybean powder for use;
[0036] (2) Add soybean powder to a phosphate buffer with a pH of 5.2 and a molar concentration of 0.05 mol / L, and stir well to prepare a mixed solution I. The volume of phosphate buffer corresponding to each gram of soybean powder is 10 mL;
[0037] (3) Put the mixture I prepared in step (2) into a centrifuge with a rotation speed of 6000r / min and centrifuge for 30 minutes, and then dissolve the precipitate in a phosphate buffer with a pH of 6.5 and a molar concentration of 0.05mol / L Then transfer to a centrifuge with a speed of 4000r / min and centrifuge for 15 minutes, collect the supernatant, and prepare the soybean extract for use;
[0038] Step 2: Weigh each component according to the weight...
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[0041] Example 1
[0042] A method for preparing a winter raw vegetable substrate using municipal sludge includes the following steps:
[0043] Step 1. Add acetic acid with a molar concentration of 0.7 mol / L and rotten citrus to the municipal sludge, stir evenly, treat with 20kHz ultrasonic waves for 25 minutes, and air-dry to obtain the substrate I, which is added per liter of municipal sludge The masses of acetic acid and citrus are 32g and 34g respectively;
[0044] Step 2. Grind the wheat straw and the air-dried substrate I, and filter them with a sieve with a pore size of 1-2 mm. Mix the filtered wheat straw and the substrate I in a 4:1 mass ratio, and mix them evenly. Matrix II, spare;
[0045] Step 3. Add water to substrate II to make the mass percentage concentration of water in substrate II 68-72%, then add microbial starter to substrate II, and the ratio of substrate II to microbial starter is 5:1, and mix The unit of matrix I is m 3 , The unit of the microbial starter is ...
Example Embodiment
[0057] Example 2
[0058] A method for preparing a winter raw vegetable substrate using municipal sludge includes the following steps:
[0059] Step 1. Add acetic acid with a molar concentration of 0.7 mol / L and rotten citrus to the municipal sludge, stir evenly, treat with 20kHz ultrasonic waves for 25 minutes, and air-dry to obtain the substrate I, which is added per liter of municipal sludge The masses of acetic acid and citrus are 32g and 34g respectively;
[0060] Step 2. Grind the wheat straw and the air-dried substrate I, and filter them with a 2mm sieve. The filtered wheat straw and the substrate I are mixed according to the mass ratio of 4:1, and the substrate II is obtained after stirring. ,spare;
[0061] Step 3. Add water to substrate II to make the mass percentage concentration of water in substrate II 68-72%, then add microbial starter to substrate II, and the ratio of substrate II to microbial starter is 5:1, and mix The unit of matrix I is m 3 , The unit of the micro...
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