Method for preparing matrix of raw winter vegetables with municipal sludge
A technology of municipal sludge and matrix, applied in the biological field, can solve the problems of municipal sludge and achieve the effects of improving absorption and utilization, simple preparation process, and reducing heating and fermentation time
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preparation example Construction
[0033] The preparation method of microbial fermentation agent described in step 3 comprises the following steps:
[0034] Step 1, preparation of soybean extract
[0035] (1) Wash the soybeans, dry them, crush them, and then filter them through a 1mm-diameter sieve to obtain soybean powder for later use;
[0036] (2) Add soybean powder into a phosphate buffer solution with a pH of 5.2 and a molar concentration of 0.05 mol / L, stir evenly to obtain a mixed solution I, and the volume of the phosphate buffer solution corresponding to each gram of soybean powder is 10 mL;
[0037] (3) Put the mixture I prepared in step (2) into a centrifuge with a rotation speed of 6000r / min for 30min, and then dissolve the precipitate in a phosphate buffer solution with a pH of 6.5 and a molar concentration of 0.05mol / L In, then transferred to a centrifuge with a rotating speed of 4000r / min for 15min, the supernatant was collected, and the soybean extract was prepared for subsequent use;
[0038]...
Embodiment 1
[0042] A kind of method utilizing city sludge to prepare winter raw vegetable matrix, comprises the following steps:
[0043] Step 1: Add 0.7 mol / L acetic acid and rotten citrus to the urban sludge, stir evenly, and then treat it with an ultrasonic wave with a frequency of 20kHz for 25 minutes, air-dry to obtain the matrix I, and add The quality of acetic acid and orange is respectively 32g, 34g;
[0044] Step 2: Crush the wheat straw and the air-dried matrix I, and filter them through a sieve with a pore size of 1-2 mm, mix the filtered wheat straw and matrix I at a mass ratio of 4:1, and stir evenly to obtain Matrix II, spare;
[0045] Step 3, add water to the matrix II, so that the mass percentage concentration of water in the matrix II is 68-72%, and then add the microbial starter to the matrix II, and the ratio of the matrix II to the microbial starter is 5:1, and mix The unit of matrix I is m 3 , the unit of the microbial fermentation agent is kg, and the substrate II...
Embodiment 2
[0058] A kind of method utilizing city sludge to prepare winter raw vegetable matrix, comprises the following steps:
[0059] Step 1: Add 0.7 mol / L acetic acid and rotten citrus to the urban sludge, stir evenly, and then treat it with an ultrasonic wave with a frequency of 20kHz for 25 minutes, air-dry to obtain the matrix I, and add The quality of acetic acid and orange is respectively 32g, 34g;
[0060] Step 2: Crush the wheat straw and the air-dried matrix I, and filter them through a sieve with a pore size of 2 mm, mix the filtered wheat straw and matrix I at a mass ratio of 4:1, and mix well to obtain matrix II ,spare;
[0061] Step 3, add water to the matrix II, so that the mass percentage concentration of water in the matrix II is 68-72%, and then add the microbial starter to the matrix II, and the ratio of the matrix II to the microbial starter is 5:1, and mix The unit of matrix I is m 3 , the unit of the microbial fermentation agent is kg, and the substrate III is ...
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