Culture method of porcine parvovirus

A technology of parvovirus and culture method, applied in virus/bacteriophage, biochemical equipment and methods, microorganisms, etc., can solve problems such as difficulty in producing high-quality vaccines, achieve good social benefits and application prospects, large growth, and simple process Effect

Inactive Publication Date: 2014-06-18
QINGDAO ZHONGREN PHARMA
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  • Summary
  • Abstract
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Problems solved by technology

[0007] In order to overcome the above-mentioned defects existing in the prior art field, the purpose of the present invention is to provide a method for culturing porcine parvovirus, which solves the problem of the time of occurrence of pathological changes due to the different propagation effects of porcine parvovirus on diffe

Method used

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Embodiment

[0018] The culture method of the porcine parvovirus of the present embodiment may further comprise the steps:

[0019] (1) Cell subculture, digest well-grown ST cells that are full of cell flasks with trypsin-EDTA solution, add MEM cell culture medium after the cells are loose, blow the digested cells evenly with a straw, pour Take 4 / 5 of the cell suspension, and then add the same amount of cell culture medium, that is, dilute the cell density to 5 times;

[0020] (2) For virus proliferation, 2% of PPV virus seeds were inoculated into ST cells simultaneously, and uninfected normal control cells were set at the same time, and placed at 37°C and 5% CO 2 Culture in an incubator, replace with serum-free MEM cell culture medium 16 hours after inoculation, observe cell lesion CPE every day and record the lesion situation, when the cells show 80% CPE, collect the toxin, freeze and thaw 3 times, 1 500 r min -1 Centrifuge for 15 min, divide the supernatant into the vials for later us...

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Abstract

The invention discloses a culture method of porcine parvovirus; porcine testis passage cells (ST) are used as a cell source for culturing the porcine parvovirus, and while cell passage, the virus is inoculated, so that multiplication culture of the virus is realized. The method has the characteristics of simple technology, high increment, high yield and low cost, and a vaccine prepared by use of the porcine parvovirus cultured by the method has a complete preventive effect on the porcine parvovirus, and has good social benefits and application prospects.

Description

[0001] technical field [0002] The invention belongs to the technical field of porcine parvovirus vaccine production, in particular to a method for cultivating porcine parvovirus. [0003] Background technique [0004] Porcine parvovirus (PPV) belongs to the Parvoviridae family and is one of the main pathogens that cause reproductive disorders in sows. Piglets died in large numbers. Pigs are the only susceptible animals to this disease, and domestic pigs and wild boars of different ages and sexes can be infected. Virus-infected pigs are the main source of infection of PPV, and the virus in the secretions and excreta of infected pigs can maintain infection for several months. Experiments have shown that although the detoxification time of infected pigs is only 1 to 2 weeks, the pens contaminated by it are still infectious for at least 4 months. This is mainly because PPV is stable to heat and is resistant to many commonly used disinfectants. Resistant, therefore, contami...

Claims

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Application Information

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IPC IPC(8): C12N7/00
Inventor 张述智夏伟朱绍辉张浩王晓丽徐权汗李之详许团辉
Owner QINGDAO ZHONGREN PHARMA
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