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Compound composition of eugenol and cnidium lactone and application thereof

A compound composition, eugenol technology, applied in the application, biocide, animal repellent and other directions, to achieve the effect of obvious control effect, reduce input and avoid drug resistance

Active Publication Date: 2014-06-25
SHANDONG YANTAI AGRI SCI & TECH INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Plant-derived pesticides eugenol and osthole are attracting more and more attention in production, but there are no reports on the prevention and treatment of apple ring spot and leaf spot leaf spot with eugenol and its compound composition with osthole

Method used

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  • Compound composition of eugenol and cnidium lactone and application thereof
  • Compound composition of eugenol and cnidium lactone and application thereof
  • Compound composition of eugenol and cnidium lactone and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Indoor toxicity test and compound ratio screening

[0029] 1.1 Drugs to be tested

[0030] 94.7% osthole, provided by the Institute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences. Soluble in 95% alcohol, dubbed 10000mg / L mother liquor for later use.

[0031] 0.3% eugenol soluble solution, product of Shandong Yijia Agrochemical Co., Ltd., dissolved in water to make 1000 mg / L mother liquor for later use.

[0032] 1.2 Tested strains and host plants

[0033] Bsotryophaeria dothidea was isolated from diseased fruit in the field, and the first-generation strain obtained by monospore culture and purification was stored in a 4°C refrigerator for later use.

[0034] 1.3 Experimental method

[0035] The Mycelium growth rate test recommended by the FAO was used.

[0036] 1.3.1 Preparation of drug-containing tablet

[0037] 1.3.1.1 Drug compounding scheme

[0038] Table 1 Compound ratio of osthole and eugenol

[0039]

[0040] Table 2 Serial dilutio...

Embodiment 2

[0070] field plot experiment

[0071] According to the indoor compound ratio screening experiment, the optimum ratio of eugenol:osthole=1:20 was selected, and we prepared a 2.1% eugenol·osthole compound composition.

[0072] 2.1 Experimental drug and community arrangement

[0073] Design 2.1% eugenol according to ""Pesticide Field Efficacy Test Guidelines (2)" Part 118: Fungicides Control Apple Ringworm and Part 124: Fungicides Control Apple Spot Leaf Blight edited by the Pesticide Control Institute of the Ministry of Agriculture · Field efficacy test of osthole on apple ring spot and apple spot defoliation. 2.1.1 Drugs to be tested

[0074] 2% osthole water EC 300 times, the product of Wuhan Tianhui Biological Engineering Co., Ltd.

[0075] 0.3% eugenol soluble solution 800 times, product of Shandong Yijia Agrochemical Co., Ltd.

[0076] 2.1% eugenol · osthole 400, 500, 600 times, prepared by Yantai Academy of Agricultural Sciences.

[0077] 2.1.2 Control drug

[0078] ...

Embodiment 3

[0108] safety test

[0109] The toxicity of the compound composition to humans and animals and the safety to crops meet the requirements; the content of osthole in the compound composition is 2%, and the content of eugenol is 0.1%. Bedsin EC and 0.3% eugenol water solvent belong to slightly toxic pesticides:

[0110] Acute oral toxicity in rats ♀>5000mg / kg;

[0111] Acute dermal toxicity in rats ♀>5000mg / kg;

[0112] Rabbit eye irritation score is 0 (1h), 0 (1:100) dilution after 48h, no irritation to eyes;

[0113] Rabbit eye irritation score is 0 (4h), no irritation to skin.

[0114] The above examples have established the optimum proportioning ratio of the two biological source pesticides through indoor toxicity determination and field tests on the effect of the biological source pesticide eugenol and osthole after being used alone and compounded on apple rings and leaf spot. For production use, in order to achieve the purpose of reducing environmental pollution fro...

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Abstract

The invention discloses application of a compound composition of eugenol and cnidium lactone in the field of agriculture and in particular relates to application research aiming at prevention and treatment of apple ring spot and alternaria leaf spot. The invention provides application of the compound composition of eugenol and cnidium lactone in prevention and treatment of the apple ring spot and the alternaria leaf spot. A weight ratio of the eugenol to cnidium lactone, in the compound composition is 10:1-1:60, a synergistic ratio is 5:1-1:40, and an optimal ratio is 1:20. The compound composition has outstanding advantages when being applied to aspects of preventing and treating the apple ring spot and the alternaria leaf spot.

Description

technical field [0001] The invention relates to a compound composition containing eugenol, which belongs to the technical field of agricultural fungicides. Background technique [0002] Apple is one of the main varieties of fruit trees planted in my country. With the continuous expansion of its cultivation area, the problems of diseases and insect pests in apple production have become increasingly prominent, especially in recent years. increasingly severe and widespread. Apple ring spot disease mainly harms branches and fruits, and can cause a large number of rotten fruits in severe cases. The fruit rot rate is generally 20-50%, and the severe one can reach 70-90%. In severe cases, it often causes early deciduous apple leaves and tree vigor to weaken, affecting yield and flower bud formation. The above two diseases belong to the main diseases in various apple producing areas in my country at present, directly affecting the economic income of fruit farmers. [0003] The app...

Claims

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Application Information

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IPC IPC(8): A01N43/16A01P3/00A01N31/16
Inventor 李宝燕王英姿刘保友王培松张伟
Owner SHANDONG YANTAI AGRI SCI & TECH INST
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