Method for culturing microalgae by using coking wastewater

A technology for coking wastewater and microalgae, which is applied in the directions of microorganism-based methods, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problems of high cost of microalgae cultivation, improve the growth rate of microalgae, and reduce industrialized cultivation. cost effect

Inactive Publication Date: 2014-06-25
中国中化股份有限公司 +1
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Problems solved by technology

The key to solving the problem of high cost of microalgae cultivation is to increase the oil content of microalgae and conduct high-density culture of microalgae or reduce the cost of materials in the process of microalgae cultivation or improve the cultivation technol...

Method used

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Embodiment

[0016] In an 800ml tubular photobioreactor, add 282ml of seawater Isochrysis zhanjiang culture medium (artificial seawater, salinity 2.6%) with an algae cell density of 8 million / ml, and then add 18ml of coking wastewater, the total volume 300ml; the control sample was cultured with artificial seawater with a salinity of 2.6%; 3 samples were cultured with coking wastewater. The culture conditions are: 25°C, pH value 8.2, carbon dioxide 8mL / min, air 0.4vvm, fluorescent lamp irradiation, light 10000lx, light-dark ratio 14h:10h. After culturing until the 13th day, the algae body entered the stable period, and the algae body was collected by centrifugation. The collected algae body was extracted with chloroform / methanol (2:1, v:v) method to analyze the oil content of the algae body.

[0017] The experimental results showed that the growth rate of microalgae cultured in coking wastewater was significantly higher than that of microalgae cultured in seawater, and the algal cell conce...

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Abstract

The present invention relates to a method for culturing microalgae by using coking wastewater, wherein a proper amount of coking wastewater is added to a microalgae culture medium, and the microalgae cells are cultured until achieving a stable growth stage so as to harvest the microalgae. According to the present invention, the results show that the growth rate of the microalgae in seawater added with the coking wastewater is higher than the growth rate of the microalgae only in seawater, and the total nitrogen of the coking wastewater is effectively degraded; and the microalgae culture and the coking wastewater treatment are combined, such that the microalgae production cost can be effectively reduced, the coking wastewater treatment environmental benefits are achieved while the microalgae is cultured, the microalgae industrial culture cost is easily reduced, and the algae bio-energy industrialization is promoted.

Description

technical field [0001] The invention belongs to the field of microalgae bioenergy, relates to a microalgae cultivation technology, in particular to a method for cultivating microalgae by using coking wastewater. Background technique [0002] In recent years, with the rapid growth of the global economy, the consumption of fossil energy such as oil and coal has risen sharply, and the crisis of shortage of fossil energy is imminent, and attention to renewable energy such as biomass energy has gradually become a hot spot. Among the many non-food biomass, algae has the characteristics of wide distribution, high oil content, strong environmental adaptability, short growth cycle, and high yield. The use of algae to prepare biofuels has great development prospects. As a greenhouse gas, carbon dioxide has been restricted globally since the Kyoto Protocol came into effect. How to reduce carbon dioxide emissions and utilize it as a resource has also become a research hotspot. Algae can...

Claims

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Application Information

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IPC IPC(8): C12N1/12C02F3/32C12R1/89
Inventor 胥维昌章表明李海龙朱希坤龙惊惊
Owner 中国中化股份有限公司
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