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Antisense oligodeoxynucleotide for micro RNA-155 seed sequence and application

A technology of RNA-155 and antisense oligonucleotides, applied in the field of antisense oligonucleotides, can solve the problems of G1 phase arrest and unreported effects

Inactive Publication Date: 2014-06-25
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This means that miR-155 causes G1 phase arrest in WM cells by regulating cyclins
So far, the role of miR-155 in multiple myeloma has not been reported

Method used

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  • Antisense oligodeoxynucleotide for micro RNA-155 seed sequence and application
  • Antisense oligodeoxynucleotide for micro RNA-155 seed sequence and application
  • Antisense oligodeoxynucleotide for micro RNA-155 seed sequence and application

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Embodiment 1

[0027] 1. Experimental materials and methods

[0028] 1. Design and synthesis of antisense oligonucleotides

[0029] The seed sequence of human microRNA-155 was obtained from the microRNA Families database, and the antisense oligonucleotide sequence (t-antimiR-155) targeting the seed sequence was designed according to the principle of sequence complementarity, and the antisense oligonucleotide sequence was determined by BLAST software analysis Acid sequences and random control sequences (such as figure 1 shown).

[0030] 2. Main reagents

[0031] MTT (tetramethylazolazolium blue) and DMSO (dimethyl sulfoxide) were purchased from Sigma; fetal bovine serum and RPMI-1640 medium were purchased from GIBCO; Lipofectamine TM 2000 was purchased from Invitrogen. RPMI-8266 cell line was purchased from Shanghai Baili Biotechnology Co., Ltd. Antisense oligonucleotide sequence (t-antimiR-155): 5′-AGCATTAA-3′; random sequence (scramble, SCR):

[0032] 5′-TCATACTA-3′, all synthesized ...

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Abstract

The invention discloses an antisense oligodeoxynucleotide for a micro RNA-155 seed sequence and an application. Micro antisense nucleic acids t-antimiR-155 of eight basic groups are designed and synthesized for the miR-155 seed sequence. T-antimiR-155 transfection cell test shows that t-antimiR-155 is targeted to miRNA-155 of a RPMI-8266 cell strain, so that cell growth is remarkably inhibited and apoptosis is remarkably increased. The test shows that t-antimiR-155 can effectively inhibit growth of lymphoma cells by interfering expression of the miRNA-155, and meanwhile, the test shows that the miRNA-155 can probably be taken as a potential target spot for gene therapy of lymphoma. The t-antimiR-155 can be used for preparing anti-tumor medicines, and particularly medicines for treating multiple myeloma.

Description

technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to an antisense oligonucleotide (tiny anti-mR-155, t-antimiR-155) targeting microRNA-155 (microRNA, miRNA) seed sequence and its application. Background technique [0002] Multiple myeloma (MM) is a malignant plasma cell clonal proliferative disease, and its pathogenesis involves multiple aspects, including abnormal chromosome number, translocation, gene mutation, epigenetic changes and changes in the bone marrow microenvironment . The onset of multiple myeloma presents a multi-step process. Early chromosomal translocations involve chromosome 14 (14q32.33) and chromosome 4 (4p16.3), resulting in adjacent MMSET (multiple myeloma SET) on chromosome 4. protein) gene and fibroblast growth factor receptor 3 (fibroblast growth factor receptors, FGFR3) gene were separated. As the disease progresses, recurrent chromosomal translocations, mutations in related genes (involving a...

Claims

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Application Information

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IPC IPC(8): C12N15/113A61K48/00A61P35/00
Inventor 费嘉骆小闯丰茂晓古春明
Owner JINAN UNIVERSITY
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