A plant drought stress-induced expression promoter posdro2 and its application
A drought stress-induced expression technology, applied in the field of plant drought stress-induced expression promoters, to achieve the effects of improving growth characteristics and drought resistance, high biological safety, and increasing expression
Inactive Publication Date: 2015-10-28
RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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Problems solved by technology
However, the drought-induced expression promoter has not been isolated in the important crop rice for plant stress resistance genetic engineering
[0004] In addition, the previous experimental evidence on using stress-induced genes such as drought and their promoters to improve plant stress resistance is basically based on the results obtained from model plants such as Arabidopsis or tobacco, and these results can all be successfully obtained. It is rarely reported in cereal crops
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[0029] The present invention will be described below with reference to specific examples. Those skilled in the art can understand that these examples are only used to illustrate the present invention and do not limit the scope of the present invention in any way.
[0030] The experimental methods in the following examples are conventional methods unless otherwise specified. The medicinal raw materials, reagent materials, etc. used in the following examples are all commercially available products unless otherwise specified.
[0031] Acquisition of PosDro2 Promoter Containing Restriction Sites
[0032] Step 1. Design of primers
[0033] According to the whole genome sequence of rice variety Nipponbare (Oryza sativa L cv. Nipponbare) provided by NCBI, the amplification primers were designed according to the sequence of rice PosDro2 gene, and the enzyme cutting sites of the primers were designed according to the characteristics of the selected vector and target gene. In this embo...
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The invention provides a plant drought stress inducible expression promoter PosDro2 and an application thereof. The invention also provides an expression cassette, a plant expression vector, host bacteria and a converter containing the promoter. According to the invention, the promoter is applied to plant transgenic engineering. A rice promoter PosDro2 cloned in the invention can be used for regulating and controlling concentrative expression of genes in a plant under a drought stress induction condition and has a remarkable value in actual application. Through use of the promoter, the gene of a crop variety is modified, for instance, the promoter is used for regulating and controlling the expression of a target gene in the plant under the drought stress induction condition, so that the growth characteristics and the drought resistance ability of the rice can be increased and improved; the promoter is used for replacing a constitutive promoter such as 35S and the like, thus an ideal transgenic drought-enduring plant variety with high biosafety can be cultivated.
Description
technical field [0001] The invention relates to the technical fields of biotechnology and plant genetic engineering. Specifically, the present invention relates to a plant drought stress-induced expression promoter and its application. The promoter can drive the expression of a target gene in a plant under drought conditions in a rice transgene regulation system. Background technique [0002] The impact of environmental changes on agricultural production is becoming more and more serious. Harsh environmental conditions, especially during the crop filling and fruiting stages, can almost make farmers lose their harvest and cause major economic losses. Plants grow in fixed environmental conditions and need to adapt to changes in environmental conditions, so that their growth and development process can adapt to the environmental conditions in which they live. The ability to adapt to changes in environmental conditions is crucial for plants. [0003] Abiotic stresses such as dr...
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IPC IPC(8): C12N15/113C12N15/82C12N1/21A01H5/00
Inventor 李浩杨剑波魏鹏程张银萍李莉秦瑞英杨亚春马卉倪大虎倪金龙
Owner RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI