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Bock greenbrier rhizome polysaccharide extract and preparation method thereof

A technique for polysaccharide extraction and extraction, applied in the field of polysaccharide extract and its preparation

Active Publication Date: 2014-07-02
四川省天府香疗健康技术研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no relevant report on the polysaccharide components of King Kongteng

Method used

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  • Bock greenbrier rhizome polysaccharide extract and preparation method thereof
  • Bock greenbrier rhizome polysaccharide extract and preparation method thereof
  • Bock greenbrier rhizome polysaccharide extract and preparation method thereof

Examples

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preparation example Construction

[0039] The preparation of embodiment 1 King Kong vine polysaccharide extract

[0040] Weigh 100g of the medicinal material of King Kongteng, put it in a 2000ml round bottom flask, add 8 times the amount of 75% ethanol solution, soak overnight, heat and reflux in a water bath for extraction for 2 hours, filter, then add 8 times the amount of 75% ethanol for reflux extraction for 2 hours, filter Then add 8 times the amount of 75% ethanol for reflux extraction for 1 hour, filter, evaporate the residue to dry the solvent, then add 12 times the amount of water, heat and reflux in a water bath at 80°C for 2 hours, filter, and then add 12 times the amount of water , heat and reflux for extraction for 2 hours, repeat again, filter, combine the extracts, centrifuge, and concentrate the supernatant to 250ml under reduced pressure (that is, the concentration of the medicinal solution is 0.4g raw medicinal materials / ml).

[0041] Add 4 times the amount of 95% ethanol to the concentrated s...

Embodiment 5

[0272] The investigation of embodiment 5 degreasing treatment

[0273] Weigh the medicinal materials of the same quality respectively, add 10 times the amount of methanol, 80% ethanol, petroleum ether (60-90℃), acetone, n-hexane, petroleum ether (60-90℃)-acetone (1:1, V / V) and chloroform-methanol (2:1, V / V), heated and refluxed for 3 times, each time for 1.5h, filtered, dried the filter residue, and then added 10 times of water and heated to reflux Extract 3 times, 1.5h each time, filter, collect the extracts respectively, concentrate under reduced pressure to the same volume, add 4 times the volume of 95% ethanol, precipitate with alcohol for 24 hours, centrifuge, collect the precipitate, dry under reduced pressure at low temperature, and obtain each Sample powder, spare.

[0274] Weigh 16 mg of sample powder from each group, put it in a 50ml volumetric flask, add a certain amount of water, ultrasonicate for 30 minutes, dilute the volume with water to the mark, shake well, f...

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Abstract

The invention provides a bock greenbrier rhizome polysaccharide extract and a preparation method thereof. The polysaccharide content of the extract is greater than 20%w / w. The invention also provides a preparation method and application of the extract. The research of the invention discovers that the bock greenbrier rhizome polysaccharide has favorable action of resisting inflammation, activating blood circulation to dissipate blood stasis and enhancing immunity and has favorable therapeutic action on pelvic inflammation.

Description

technical field [0001] The invention relates to an extract of polysaccharides from vine vine and a preparation method thereof. Background technique [0002] With the acceleration of people's life rhythm and the improvement of economic level, people's requirements for quality of life are also getting higher and higher. Some chronic diseases will not directly lead to life-threatening, but due to the long-term lingering and repeated attacks of the disease, it will also bring inconvenience and discomfort to people's lives. Chronic pelvic inflammatory disease is one of these diseases. Chronic pelvic inflammatory disease ( Chronic pelvic inflammatory disease (CPID) refers to the chronic inflammation of female reproductive organs, surrounding connective tissue and pelvic peritoneum. It is one of the most common genital uterine inflammations in women of childbearing age. Chronic pelvic inflammatory disease can lead to infertility, ectopic pregnancy, ectopic pregnancy, other gynecol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P15/00A61P29/00A61P37/04
Inventor 谭周菊
Owner 四川省天府香疗健康技术研究院有限公司
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