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Nucleic acid automatic extraction device and method based on nano magnetic beads

A technology of automatic extraction and nano-magnetic beads, which is applied in the methods of stress-stimulated microbial growth, biochemical cleaning devices, biochemical equipment and methods, etc., can solve the problems of complex operation, difficult to achieve automation, and needs, and achieve consistency. , the effect of reducing human interference and reducing the risk of cross-interference

Inactive Publication Date: 2016-05-04
CHINA AGRI UNIV
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Problems solved by technology

[0003] With the development of molecular biotechnology and the improvement of scientific research and clinical testing requirements, nucleic acid purification methods have also undergone chemical and physical methods such as density gradient centrifugation, phenol / chloroform, phenol, isoamyl alcohol extraction, and silica adsorption. Complicated, requires centrifugation, not easy to automate

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  • Nucleic acid automatic extraction device and method based on nano magnetic beads
  • Nucleic acid automatic extraction device and method based on nano magnetic beads
  • Nucleic acid automatic extraction device and method based on nano magnetic beads

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Embodiment Construction

[0038] Embodiments of the present invention will be further described in detail below in conjunction with the accompanying drawings and examples. The following examples are used to illustrate the present invention, but should not be used to limit the scope of the present invention.

[0039] Such as figure 1 As shown, the nucleic acid extraction device based on nano-magnetic beads in this embodiment includes a base 1 and three linear transmission structures installed on the base. The first stepper motor 71 links to each other with the first screw mandrel 91 through the first coupling 111, and the magnetic bar clamping mechanism 3 links to each other with the first screw mandrel 91. When the motor rotates, according to the different directions of rotation, the magnetic bar clamping mechanism 3 It can move back and forth along the first slide bar 81 , so that the magnetic bar 6 can move up and down. The second stepper motor 72 connects the second screw mandrel 92 through the se...

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Abstract

The invention provides an automatic extraction device and an automatic extraction method of a nucleic acid based on nano magnetic beads. The device comprises a base (1), as well as a motor, a magnetic bar (6), a magnetic bar clamping mechanism (3), a stirring sleeve (5), a stirring sleeve clamping mechanism (10), a deep hole plate (2), a control circuit and an upper computer which are arranged on the base (1). According to the device and the method which are provided by the invention, the nucleic acid is combined with the nano magnetic beads; and then, the nucleic acid is extracted from a nucleic acid cracking liquid under the attraction of the magnetic bar (6). Thus, the consistency of sample treatment is guaranteed to a great extent; the human interference is reduced; and the cross interference risk is decreased. The device and the method provided by the invention are suitable for the pretreatment of sample inspection in the medicinal field, the biological field, the agricultural field and the like.

Description

technical field [0001] The invention relates to the field of nucleic acid extraction, in particular to an automatic nucleic acid extraction technology based on nano magnetic beads. Background technique [0002] The isolation and extraction of nucleic acids (DNA and RNA) is a difficult and onerous task in biotechnology. Nucleic acid extraction is inseparable from microbial virus detection, gene screening and recombination, clinical diagnosis and treatment. It is the basis of downstream nucleic acid detection and scientific research, and the quality and integrity of the extracted nucleic acid will directly affect the results of research or diagnosis. [0003] With the development of molecular biotechnology and the improvement of scientific research and clinical testing requirements, nucleic acid purification methods have also undergone chemical and physical methods such as density gradient centrifugation, phenol / chloroform, phenol, isoamyl alcohol extraction, and silica adsor...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/42C12M1/38C12M1/36C12M1/34C12M1/02C12N15/10
CPCC12N15/1013
Inventor 李莉江琴林建涵张彦娥王禹贺陈雅慧
Owner CHINA AGRI UNIV
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